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题名: Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China
作者: Liu, Yang1, 2; Xu, Yao1; Xiao, Peng1, 2; Pan, Qianqian1, 2; Yu, Gongliang1; Li, Renhui1
通讯作者: Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
关键词: Cyanobacteria ; Dolichospermum ; Genotype ; ITS sequence ; Lake Taihu
刊名: HARMFUL ALGAE
发表日期: 2014
DOI: 10.1016/j.hal.2013.09.012
卷: 31, 页:76-81
收录类别: SCI
文章类型: Article
部门归属: [Liu, Yang; Xu, Yao; Xiao, Peng; Pan, Qianqian; Yu, Gongliang; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China; [Liu, Yang; Xiao, Peng; Pan, Qianqian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
类目[WOS]: Marine & Freshwater Biology
研究领域[WOS]: Marine & Freshwater Biology
摘要: Lake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: Lake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: REAL-TIME PCR ; PROCHLOROCOCCUS ECOTYPES ; MICROCYSTIS-AERUGINOSA ; SPATIOTEMPORAL CHANGES ; ENVIRONMENTAL-FACTORS ; TOXIC MICROCYSTIS ; FLOS-AQUAE ; BLOOMS ; DIVERSITY ; DYNAMICS
语种: 英语
WOS记录号: WOS:000330497500009
ISSN号: 1568-9883
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20120
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Yang; Xu, Yao; Xiao, Peng; Pan, Qianqian; Yu, Gongliang; Li, Renhui.Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China,HARMFUL ALGAE,2014,31():76-81
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