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Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China
Liu, Yang1,2; Xu, Yao1; Xiao, Peng1,2; Pan, Qianqian1,2; Yu, Gongliang1; Li, Renhui1; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2014
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume31Pages:76-81
AbstractLake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved.; Lake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordCyanobacteria Dolichospermum Genotype Its Sequence Lake Taihu
Department[Liu, Yang; Xu, Yao; Xiao, Peng; Pan, Qianqian; Yu, Gongliang; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China; [Liu, Yang; Xiao, Peng; Pan, Qianqian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.hal.2013.09.012
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000330497500009
WOS KeywordREAL-TIME PCR ; PROCHLOROCOCCUS ECOTYPES ; MICROCYSTIS-AERUGINOSA ; SPATIOTEMPORAL CHANGES ; ENVIRONMENTAL-FACTORS ; TOXIC MICROCYSTIS ; FLOS-AQUAE ; BLOOMS ; DIVERSITY ; DYNAMICS
Funding OrganizationNSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20120
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yang,Xu, Yao,Xiao, Peng,et al. Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China[J]. HARMFUL ALGAE,2014,31:76-81.
APA Liu, Yang.,Xu, Yao.,Xiao, Peng.,Pan, Qianqian.,Yu, Gongliang.,...&Li, RH .(2014).Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China.HARMFUL ALGAE,31,76-81.
MLA Liu, Yang,et al."Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China".HARMFUL ALGAE 31(2014):76-81.
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