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题名: Effects of light intensity on components and topographical structures of extracellular polymeric substances from Microcoleus vaginatus (Cyanophyceae)
作者: Ge, Hongmei1, 2; Zhang, Jing3; Zhou, Xuping1, 2; Xia, Ling1, 2; Hu, Chunxiang1
通讯作者: Hu, CX (reprint author), Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Atomic force microscopy ; Biological soil crusts ; Capsular polysaccharides (CPS) ; Cyanobacteria ; Light intensity ; Released polysaccharides (RPS)
刊名: PHYCOLOGIA
发表日期: 2014
DOI: 10.2216/13-163.1
卷: 53, 期:2, 页:167-173
收录类别: SCI
文章类型: Article
部门归属: [Ge, Hongmei; Zhou, Xuping; Xia, Ling; Hu, Chunxiang] Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Ge, Hongmei; Zhou, Xuping; Xia, Ling] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China; [Zhang, Jing] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31170464, 30870470]; National Forestry Public Welfare Industry Research Project [201404204]
类目[WOS]: Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]: Plant Sciences ; Marine & Freshwater Biology
摘要: Extracellular polymeric substances (EPS) acting as cementing substance (chelator) of biological soil crusts (BSCs) are a considerable amount of metabolic investment for cyanobacteria. However, their partition mechanism has long been overlooked. In this paper, we investigated the effects of light intensity on production, distribution pattern, carbohydrate and protein contents, monosaccharide composition, and topographical structures of EPS from Microcoleus vaginatus, the first dominant cyanobacterium of BSCs. The results showed that the amounts of released polysaccharides (RPS), capsular polysaccharides (CPS), and total EPS increased with enhanced light intensity. The weaker the light, the higher the ratio of CPS to EPS and the lower the ratio of RPS to EPS. Although the highest light intensity significantly reduced the charge distribution in RPS, it had no effect on the charge distribution of CPS. For monosaccharide composition, although light intensity did not influence either RPS or CPS, the proportions of main components were still different. Carbohydrate, glucose, galactose, and arabinose were most abundant in RPS; whereas, glucose and galactose were highest in CPS. Comparison with protein content in RPS, carbohydrate was dominant in water eluates under 40 mu E m(-2) s(-1), whereas protein was dominant in 1.0 M NaCl eluates. Carbohydrate was dominant in both eluates under 80 mu E m(-2) s(-1). Protein content of the same fraction in CPS generally decreased with increasing light intensity. The images from atomic force microscopy showed that light intensity significantly affected the molecular weight of EPS, as well as the topographical structures, namely, long chains only found in RPS at 80 mu E m(-2) s(-1) and CPS at 4 mu E m(-2) s(-1). Short chains and irregular lumps occurred under all test light intensities. The above results strongly suggested that light intensity not only affects the EPS yield but also its partition pattern, component, and spatial structure. Therefore, light intensity also likely influences BSCs gluing cohesion.
英文摘要: Extracellular polymeric substances (EPS) acting as cementing substance (chelator) of biological soil crusts (BSCs) are a considerable amount of metabolic investment for cyanobacteria. However, their partition mechanism has long been overlooked. In this paper, we investigated the effects of light intensity on production, distribution pattern, carbohydrate and protein contents, monosaccharide composition, and topographical structures of EPS from Microcoleus vaginatus, the first dominant cyanobacterium of BSCs. The results showed that the amounts of released polysaccharides (RPS), capsular polysaccharides (CPS), and total EPS increased with enhanced light intensity. The weaker the light, the higher the ratio of CPS to EPS and the lower the ratio of RPS to EPS. Although the highest light intensity significantly reduced the charge distribution in RPS, it had no effect on the charge distribution of CPS. For monosaccharide composition, although light intensity did not influence either RPS or CPS, the proportions of main components were still different. Carbohydrate, glucose, galactose, and arabinose were most abundant in RPS; whereas, glucose and galactose were highest in CPS. Comparison with protein content in RPS, carbohydrate was dominant in water eluates under 40 mu E m(-2) s(-1), whereas protein was dominant in 1.0 M NaCl eluates. Carbohydrate was dominant in both eluates under 80 mu E m(-2) s(-1). Protein content of the same fraction in CPS generally decreased with increasing light intensity. The images from atomic force microscopy showed that light intensity significantly affected the molecular weight of EPS, as well as the topographical structures, namely, long chains only found in RPS at 80 mu E m(-2) s(-1) and CPS at 4 mu E m(-2) s(-1). Short chains and irregular lumps occurred under all test light intensities. The above results strongly suggested that light intensity not only affects the EPS yield but also its partition pattern, component, and spatial structure. Therefore, light intensity also likely influences BSCs gluing cohesion.
关键词[WOS]: EXOPOLYSACCHARIDE-PRODUCING CYANOBACTERIA ; LIQUID SUSPENSION-CULTURE ; CARBOHYDRATE POLYMERS ; NOSTOC-FLAGELLIFORME ; SOIL CRUSTS ; POLYSACCHARIDES ; ALGAE
语种: 英语
WOS记录号: WOS:000336981600006
ISSN号: 0031-8884
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20119
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430070, Peoples R China

Recommended Citation:
Ge, Hongmei; Zhang, Jing; Zhou, Xuping; Xia, Ling; Hu, Chunxiang.Effects of light intensity on components and topographical structures of extracellular polymeric substances from Microcoleus vaginatus (Cyanophyceae),PHYCOLOGIA,2014,53(2):167-173
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