IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Ecological Dynamics of Toxic Microcystis spp. and Microcystin-Degrading Bacteria in Dianchi Lake, China
Zhu, Lin1,2; Wu, Yanlong1,2; Song, Lirong1; Gan, Nanqin1; Gan, NQ (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2014-03-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume80Issue:6Pages:1874-1881
AbstractToxic cyanobacterial blooms directly threaten both human safety and the ecosystem of surface waters. The widespread occurrence of these organisms, coupled with the tumor-promoting properties of the microcystin toxins that they produce, demands action to mitigate their potential impacts and, thus, a robust understanding of their ecological dynamics. In the present work, the abundance of toxic Microcystis spp. and microcystin (MC)-degrading bacteria in Dianchi Lake, located in Yunnan Province, China, was studied using quantitative PCR. Samples were taken at monthly intervals from June 2010 to December 2011 at three sampling stations within this freshwater lake. Results revealed that variation in the abundance of both total Microcystis spp. and toxic Microcystis spp. exhibited similar trends during the period of the algal bloom, including the reinvasion, pelagic growth, sedimentation, and overwintering periods, and that the proportion of toxic Microcystis was highest during the bloom and lowest in winter. Importantly, we observed that peaks in mlrA gene copy numbers of MC-degrading bacteria occurred in the months following observed peaks in MC concentrations. To understand this phenomenon, we added MCs to the MC-degrading bacteria (designated strains HW and SW in this study) and found that MCs significantly enhanced mlrA gene copy numbers over the number for the control by a factor of 5.2 for the microcystin-RR treatment and a factor of 3.7 for the microcystin-LR treatment. These results indicate that toxic Microcystis and MC-degrading bacteria exert both direct and indirect effects on each other and that MC-degrading bacteria also mediate a shift from toxic to nontoxic populations of Microcystis.; Toxic cyanobacterial blooms directly threaten both human safety and the ecosystem of surface waters. The widespread occurrence of these organisms, coupled with the tumor-promoting properties of the microcystin toxins that they produce, demands action to mitigate their potential impacts and, thus, a robust understanding of their ecological dynamics. In the present work, the abundance of toxic Microcystis spp. and microcystin (MC)-degrading bacteria in Dianchi Lake, located in Yunnan Province, China, was studied using quantitative PCR. Samples were taken at monthly intervals from June 2010 to December 2011 at three sampling stations within this freshwater lake. Results revealed that variation in the abundance of both total Microcystis spp. and toxic Microcystis spp. exhibited similar trends during the period of the algal bloom, including the reinvasion, pelagic growth, sedimentation, and overwintering periods, and that the proportion of toxic Microcystis was highest during the bloom and lowest in winter. Importantly, we observed that peaks in mlrA gene copy numbers of MC-degrading bacteria occurred in the months following observed peaks in MC concentrations. To understand this phenomenon, we added MCs to the MC-degrading bacteria (designated strains HW and SW in this study) and found that MCs significantly enhanced mlrA gene copy numbers over the number for the control by a factor of 5.2 for the microcystin-RR treatment and a factor of 3.7 for the microcystin-LR treatment. These results indicate that toxic Microcystis and MC-degrading bacteria exert both direct and indirect effects on each other and that MC-degrading bacteria also mediate a shift from toxic to nontoxic populations of Microcystis.
SubtypeArticle
KeywordReal-time Pcr Water-treatment-plant Biological Treatment Facility Gene-cluster Fresh-water Sand Filter Degradation Lr Quantification Cyanobacteria
Department[Zhu, Lin; Wu, Yanlong; Song, Lirong; Gan, Nanqin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Zhu, Lin; Wu, Yanlong] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1128/AEM.02972-13
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000332025800008
WOS KeywordREAL-TIME PCR ; WATER-TREATMENT-PLANT ; BIOLOGICAL TREATMENT FACILITY ; GENE-CLUSTER ; FRESH-WATER ; SAND FILTER ; DEGRADATION ; LR ; QUANTIFICATION ; CYANOBACTERIA
Funding OrganizationNational Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604] ; National Natural Science Foundation of China [31370418]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; Natural Science Foundation of China-Yunnan Project [U0833604]
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20112
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorGan, NQ (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Lin,Wu, Yanlong,Song, Lirong,et al. Ecological Dynamics of Toxic Microcystis spp. and Microcystin-Degrading Bacteria in Dianchi Lake, China[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(6):1874-1881.
APA Zhu, Lin,Wu, Yanlong,Song, Lirong,Gan, Nanqin,&Gan, NQ .(2014).Ecological Dynamics of Toxic Microcystis spp. and Microcystin-Degrading Bacteria in Dianchi Lake, China.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(6),1874-1881.
MLA Zhu, Lin,et al."Ecological Dynamics of Toxic Microcystis spp. and Microcystin-Degrading Bacteria in Dianchi Lake, China".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.6(2014):1874-1881.
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