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题名: Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803
作者: Chen, Gao1, 2; Qu, Shujie5; Wang, Qiang4; Bian, Fei1, 2; Peng, Zhenying1, 2; Zhang, Yan1, 2; Ge, Haitao6; Yu, Jinhui1, 2; Xuan, Ning1, 2; Bi, Yuping1, 2; He, Qingfang1, 2, 3
通讯作者: Bi, YP (reprint author), Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China.
关键词: Omega-3 polyunsaturated fatty acids ; Gene expression ; Fatty acid desaturase ; Synechocystis sp PCC6803
刊名: BIOTECHNOLOGY FOR BIOFUELS
发表日期: 2014-03-01
DOI: 10.1186/1754-6834-7-32
卷: 7
收录类别: SCI
文章类型: Article
部门归属: [Chen, Gao; Bian, Fei; Peng, Zhenying; Zhang, Yan; Yu, Jinhui; Xuan, Ning; Bi, Yuping; He, Qingfang] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China; [Chen, Gao; Bian, Fei; Peng, Zhenying; Zhang, Yan; Yu, Jinhui; Xuan, Ning; Bi, Yuping; He, Qingfang] Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China; [He, Qingfang] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA; [Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China; [Qu, Shujie] Shandong Acad Agr Sci, Test Base Serv Ctr, Jinan 250100, Peoples R China; [Ge, Haitao] Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Technology
资助者: International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153]
类目[WOS]: Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]: Biotechnology & Applied Microbiology ; Energy & Fuels
摘要: Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete. Results: We constructed a series of homologous vectors for the endogenous and exogenous expression of Delta 6 and Delta 15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9 mg/l of alpha-linolenic acid (ALA) and 4.1 mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20 degrees C and 30 degrees C. Thus, transgenic expression of Delta 6 and Delta 15 fatty acid desaturases enhances the accumulation of specific omega-3 PUFAs in Synechocystis sp. PCC6803. Conclusions: In the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and gamma-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential omega-3 PUFAs.
英文摘要: Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete.
关键词[WOS]: STEARIDONIC ACID ; LONG-CHAIN ; SOYBEAN OIL ; CYANOBACTERIUM SYNECHOCYSTIS-6803 ; CARDIOVASCULAR-DISEASE ; RHEUMATOID-ARTHRITIS ; FISH CONSUMPTION ; LINOLENIC ACID ; OILSEED CROPS ; PLANTS
语种: 英语
WOS记录号: WOS:000333014600001
ISSN号: 1754-6834
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20111
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China
3.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
5.Shandong Acad Agr Sci, Test Base Serv Ctr, Jinan 250100, Peoples R China
6.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China

Recommended Citation:
Chen, Gao; Qu, Shujie; Wang, Qiang; Bian, Fei; Peng, Zhenying; Zhang, Yan; Ge, Haitao; Yu, Jinhui; Xuan, Ning; Bi, Yuping; He, Qingfang.Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803,BIOTECHNOLOGY FOR BIOFUELS,2014,7():
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