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Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803
Chen, Gao1,2; Qu, Shujie5; Wang, Qiang4; Bian, Fei1,2; Peng, Zhenying1,2; Zhang, Yan1,2; Ge, Haitao6; Yu, Jinhui1,2; Xuan, Ning1,2; Bi, Yuping1,2; He, Qingfang1,2,3; Bi, YP (reprint author), Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China.
2014-03-01
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
Volume7
AbstractBackground: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete. Results: We constructed a series of homologous vectors for the endogenous and exogenous expression of Delta 6 and Delta 15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9 mg/l of alpha-linolenic acid (ALA) and 4.1 mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20 degrees C and 30 degrees C. Thus, transgenic expression of Delta 6 and Delta 15 fatty acid desaturases enhances the accumulation of specific omega-3 PUFAs in Synechocystis sp. PCC6803. Conclusions: In the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and gamma-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential omega-3 PUFAs.; Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete.
SubtypeArticle
KeywordOmega-3 Polyunsaturated Fatty Acids Gene Expression Fatty Acid Desaturase Synechocystis Sp Pcc6803
Department[Chen, Gao; Bian, Fei; Peng, Zhenying; Zhang, Yan; Yu, Jinhui; Xuan, Ning; Bi, Yuping; He, Qingfang] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China; [Chen, Gao; Bian, Fei; Peng, Zhenying; Zhang, Yan; Yu, Jinhui; Xuan, Ning; Bi, Yuping; He, Qingfang] Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China; [He, Qingfang] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA; [Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China; [Qu, Shujie] Shandong Acad Agr Sci, Test Base Serv Ctr, Jinan 250100, Peoples R China; [Ge, Haitao] Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
DOI10.1186/1754-6834-7-32
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
Funding OrganizationInternational Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000333014600001
WOS KeywordSTEARIDONIC ACID ; LONG-CHAIN ; SOYBEAN OIL ; CYANOBACTERIUM SYNECHOCYSTIS-6803 ; CARDIOVASCULAR-DISEASE ; RHEUMATOID-ARTHRITIS ; FISH CONSUMPTION ; LINOLENIC ACID ; OILSEED CROPS ; PLANTS
Funding OrganizationInternational Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153] ; International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153]
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20111
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorBi, YP (reprint author), Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China.
Affiliation1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China
3.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
5.Shandong Acad Agr Sci, Test Base Serv Ctr, Jinan 250100, Peoples R China
6.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
Recommended Citation
GB/T 7714
Chen, Gao,Qu, Shujie,Wang, Qiang,et al. Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803[J]. BIOTECHNOLOGY FOR BIOFUELS,2014,7.
APA Chen, Gao.,Qu, Shujie.,Wang, Qiang.,Bian, Fei.,Peng, Zhenying.,...&Bi, YP .(2014).Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803.BIOTECHNOLOGY FOR BIOFUELS,7.
MLA Chen, Gao,et al."Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803".BIOTECHNOLOGY FOR BIOFUELS 7(2014).
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