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Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2
Chen, Hui1; Zhang, Yunming1,2; He, Chenliu1; Wang, Qiang1; Wang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.
2014-03-01
Source PublicationPLANT AND CELL PHYSIOLOGY
ISSN0032-0781
Volume55Issue:3Pages:634-644
AbstractChanges in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.; Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
SubtypeArticle
KeywordCa2++ Channel Ca2++ Signal Transduction Chlorella Sp C2 Neutral Lipid Synthesis Nitrogen Starvation
Department[Chen, Hui; Zhang, Yunming; He, Chenliu; Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China; [Zhang, Yunming] Univ Chinese Acad Sci, Beijing 100094, Peoples R China
DOI10.1093/pcp/pcu015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030]
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Cell Biology
WOS SubjectPlant Sciences ; Cell Biology
WOS IDWOS:000333096200014
WOS KeywordCROSS-TALK ; CALCIUM ; EXPRESSION ; STRESS ; PLANTS ; PURIFICATION ; SPECIFICITY ; MICROALGAE ; TRANSPORT ; REDUCTASE
Funding OrganizationNational Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030] ; National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030]
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Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20110
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorWang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100094, Peoples R China
Recommended Citation
GB/T 7714
Chen, Hui,Zhang, Yunming,He, Chenliu,et al. Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2[J]. PLANT AND CELL PHYSIOLOGY,2014,55(3):634-644.
APA Chen, Hui,Zhang, Yunming,He, Chenliu,Wang, Qiang,&Wang, Q .(2014).Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2.PLANT AND CELL PHYSIOLOGY,55(3),634-644.
MLA Chen, Hui,et al."Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2".PLANT AND CELL PHYSIOLOGY 55.3(2014):634-644.
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