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题名: Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2
作者: Chen, Hui1; Zhang, Yunming1, 2; He, Chenliu1; Wang, Qiang1
通讯作者: Wang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.
关键词: Ca2+ channel ; Ca2+ signal transduction ; Chlorella sp ; C2 ; Neutral lipid synthesis ; Nitrogen starvation
刊名: PLANT AND CELL PHYSIOLOGY
发表日期: 2014-03-01
DOI: 10.1093/pcp/pcu015
卷: 55, 期:3, 页:634-644
收录类别: SCI
文章类型: Article
部门归属: [Chen, Hui; Zhang, Yunming; He, Chenliu; Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China; [Zhang, Yunming] Univ Chinese Acad Sci, Beijing 100094, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030]
类目[WOS]: Plant Sciences ; Cell Biology
研究领域[WOS]: Plant Sciences ; Cell Biology
摘要: Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
英文摘要: Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
关键词[WOS]: CROSS-TALK ; CALCIUM ; EXPRESSION ; STRESS ; PLANTS ; PURIFICATION ; SPECIFICITY ; MICROALGAE ; TRANSPORT ; REDUCTASE
语种: 英语
WOS记录号: WOS:000333096200014
ISSN号: 0032-0781
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20110
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100094, Peoples R China

Recommended Citation:
Chen, Hui; Zhang, Yunming; He, Chenliu; Wang, Qiang.Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2,PLANT AND CELL PHYSIOLOGY,2014,55(3):634-644
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