IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Phosphoproteomic Analysis of Protein Phosphorylation Networks in Tetrahymena thermophila, a Model Single-celled Organism
Tian, Miao1,2; Chen, Xiulan3; Xiong, Qian1; Xiong, Jie1; Xiao, Chuanle4; Ge, Feng1; Yang, Fuquan3; Miao, Wei1; Tian, M (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2014-02-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume13Issue:2Pages:503-519
AbstractTetrahymena thermophila is a widely used unicellular eukaryotic model organism in biological research and contains more than 1000 protein kinases and phosphatases with specificity for Ser/Thr/Tyr residues. However, only a few dozen phosphorylation sites in T. thermophila are known, presenting a major obstacle to further understanding of the regulatory roles of reversible phosphorylation in this organism. In this study, we used high-accuracy mass-spectrometry-based proteomics to conduct global and site-specific phosphoproteome profiling of T. thermophila. In total, 1384 phosphopeptides and 2238 phosphorylation sites from 1008 T. thermophila proteins were identified through the combined use of peptide prefractionation, TiO2 enrichment, and two-dimensional LC-MS/MS analysis. The identified phosphoproteins are implicated in the regulation of various biological processes such as transport, gene expression, and mRNA metabolic process. Moreover, integrated analysis of the T. thermophila phosphoproteome and gene network revealed the potential biological functions of many previously unannotated proteins and predicted some putative kinase-substrate pairs. Our data provide the first global survey of phosphorylation in T. thermophila using a phosphoproteomic approach and suggest a wide-ranging regulatory scope of this modification. The provided dataset is a valuable resource for the future understanding of signaling pathways in this important model organism.; Tetrahymena thermophila is a widely used unicellular eukaryotic model organism in biological research and contains more than 1000 protein kinases and phosphatases with specificity for Ser/Thr/Tyr residues. However, only a few dozen phosphorylation sites in T. thermophila are known, presenting a major obstacle to further understanding of the regulatory roles of reversible phosphorylation in this organism. In this study, we used high-accuracy mass-spectrometry-based proteomics to conduct global and site-specific phosphoproteome profiling of T. thermophila. In total, 1384 phosphopeptides and 2238 phosphorylation sites from 1008 T. thermophila proteins were identified through the combined use of peptide prefractionation, TiO2 enrichment, and two-dimensional LC-MS/MS analysis. The identified phosphoproteins are implicated in the regulation of various biological processes such as transport, gene expression, and mRNA metabolic process. Moreover, integrated analysis of the T. thermophila phosphoproteome and gene network revealed the potential biological functions of many previously unannotated proteins and predicted some putative kinase-substrate pairs. Our data provide the first global survey of phosphorylation in T. thermophila using a phosphoproteomic approach and suggest a wide-ranging regulatory scope of this modification. The provided dataset is a valuable resource for the future understanding of signaling pathways in this important model organism.
SubtypeArticle
KeywordGenome Database Tgd Human Liver-tissue Mass-spectrometry Saccharomyces-cerevisiae Mouse-liver In-vivo Biological Networks Gene-expression Wide Prediction Global Analysis
Department[Tian, Miao; Xiong, Qian; Xiong, Jie; Ge, Feng; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China; [Tian, Miao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Xiulan; Yang, Fuquan] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Xiao, Chuanle] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China
DOI10.1074/mcp.M112.026575
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationProjects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000331369000010
WOS KeywordGENOME DATABASE TGD ; HUMAN LIVER-TISSUE ; MASS-SPECTROMETRY ; SACCHAROMYCES-CEREVISIAE ; MOUSE-LIVER ; IN-VIVO ; BIOLOGICAL NETWORKS ; GENE-EXPRESSION ; WIDE PREDICTION ; GLOBAL ANALYSIS
Funding OrganizationProjects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China [2013DFG32390]; National Natural Science Foundation of China [91231118]; Knowledge Innovation Program of CAS [KSCX2-EW-G-6-4]; Hundred Talents Program of the Chinese Academy of Sciences; National Basic Research Program of China (973) [2010CB833703, 2012CB966803]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20095
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorTian, M (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
4.Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Tian, Miao,Chen, Xiulan,Xiong, Qian,et al. Phosphoproteomic Analysis of Protein Phosphorylation Networks in Tetrahymena thermophila, a Model Single-celled Organism[J]. MOLECULAR & CELLULAR PROTEOMICS,2014,13(2):503-519.
APA Tian, Miao.,Chen, Xiulan.,Xiong, Qian.,Xiong, Jie.,Xiao, Chuanle.,...&Tian, M .(2014).Phosphoproteomic Analysis of Protein Phosphorylation Networks in Tetrahymena thermophila, a Model Single-celled Organism.MOLECULAR & CELLULAR PROTEOMICS,13(2),503-519.
MLA Tian, Miao,et al."Phosphoproteomic Analysis of Protein Phosphorylation Networks in Tetrahymena thermophila, a Model Single-celled Organism".MOLECULAR & CELLULAR PROTEOMICS 13.2(2014):503-519.
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