IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development
Zhai, Gang1,2; Gu, Qilin1,2; He, Jiangyan1; Lou, Qiyong1; Chen, Xiaowen1; Jin, Xia1; Bi, Erfei3; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
2014-04-01
Source PublicationMOLECULAR AND CELLULAR BIOLOGY
ISSN0270-7306
Volume34Issue:7Pages:1310-1321
AbstractSeptins are conserved filament-forming GTP-binding proteins that act as cellular scaffolds or diffusion barriers in a number of cellular processes. However, the role of septins in vertebrate development remains relatively obscure. Here, we show that zebrafish septin 6 (sept6) is first expressed in the notochord and then in nearly all of the ciliary organs, including Kupffer's vesicle (KV), the pronephros, eye, olfactory bulb, and neural tube. Knockdown of sept6 in zebrafish embryos results in reduced numbers and length of cilia in KV. Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised. Knockdown of sept6 also results in aberrant cilium formation in the pronephros and neural tube, leading to cilium-related defects in pronephros development and Sonic hedgehog (Shh) signaling. We further demonstrate that SEPT6 associates with acetylated alpha-tubulin in vivo and localizes along the axoneme in the cilia of zebrafish pro-nephric duct cells as well as cultured ZF4 cells. Our study reveals a novel role of sept6 in ciliogenesis during early embryonic development in zebrafish.; Septins are conserved filament-forming GTP-binding proteins that act as cellular scaffolds or diffusion barriers in a number of cellular processes. However, the role of septins in vertebrate development remains relatively obscure. Here, we show that zebrafish septin 6 (sept6) is first expressed in the notochord and then in nearly all of the ciliary organs, including Kupffer's vesicle (KV), the pronephros, eye, olfactory bulb, and neural tube. Knockdown of sept6 in zebrafish embryos results in reduced numbers and length of cilia in KV. Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised. Knockdown of sept6 also results in aberrant cilium formation in the pronephros and neural tube, leading to cilium-related defects in pronephros development and Sonic hedgehog (Shh) signaling. We further demonstrate that SEPT6 associates with acetylated alpha-tubulin in vivo and localizes along the axoneme in the cilia of zebrafish pro-nephric duct cells as well as cultured ZF4 cells. Our study reveals a novel role of sept6 in ciliogenesis during early embryonic development in zebrafish.
SubtypeArticle
KeywordLeft-right Asymmetry Polycystic Kidney-disease Primary Cilium Saccharomyces-cerevisiae Mammalian Septins Budding Yeast Intraflagellar Transport Filament Formation Diffusion Barrier Gene Southpaw
Department[Zhai, Gang; Gu, Qilin; He, Jiangyan; Lou, Qiyong; Chen, Xiaowen; Jin, Xia; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Zhai, Gang; Gu, Qilin] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Bi, Erfei] Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
DOI10.1128/MCB.01409-13
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000332696800012
WOS KeywordLEFT-RIGHT ASYMMETRY ; POLYCYSTIC KIDNEY-DISEASE ; PRIMARY CILIUM ; SACCHAROMYCES-CEREVISIAE ; MAMMALIAN SEPTINS ; BUDDING YEAST ; INTRAFLAGELLAR TRANSPORT ; FILAMENT FORMATION ; DIFFUSION BARRIER ; GENE SOUTHPAW
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20090
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
Recommended Citation
GB/T 7714
Zhai, Gang,Gu, Qilin,He, Jiangyan,et al. Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development[J]. MOLECULAR AND CELLULAR BIOLOGY,2014,34(7):1310-1321.
APA Zhai, Gang.,Gu, Qilin.,He, Jiangyan.,Lou, Qiyong.,Chen, Xiaowen.,...&Yin, Z .(2014).Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development.MOLECULAR AND CELLULAR BIOLOGY,34(7),1310-1321.
MLA Zhai, Gang,et al."Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development".MOLECULAR AND CELLULAR BIOLOGY 34.7(2014):1310-1321.
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