IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis
Hu, Bo; Zhang, Wei; Feng, Xi; Ji, Wei; Xie, Xunwei; Xiao, Wuhan; Xiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
2014-04-01
Source PublicationDEVELOPMENTAL BIOLOGY
ISSN0012-1606
Volume388Issue:1Pages:81-93
AbstractStudies implicate a potential role for EAF1 in MLL-ELL induced leukemogenesis; however the biological function of EAF1 in this process remains unknown. In this study, we show that knockdown of zebrafish eaf1 by morpholinos caused serious defects in both primitive and definitive hematopoiesis. Using microarray analysis, we identified foxo3b as a target gene suppressed by eaf1. Ectopic expression of foxo3b in embryos mimicked the phenotypes exhibited in eafl morphants, except that foxo3b had no effect on runxl and c-myb expression while eaf1 morphants did not express these markers in the ventral wall of dorsal aorta. Subsequent experiments showed that a dominant negative form of Foxo3b (dnfoxo3b) partially restored primitive hematopoietic defects in eafl morphants, suggesting that foxo3b might serve as a key factor for mediating eafl function in primitive hematopoiesis. Furthermore, we observed that foxo3b inhibited the transcriptional activity of gata1 and spi1 through protein-protein interaction. Our findings not only suggest a function of eafl on hematopoiesis in vivo, but also reveal a novel regulatory pathway, eaf1-foxo3b-gata1/spi1, that may shed light on the role of EAF1 in MLL-ELL induced leukemogenesis. (C) 2014 Elsevier Inc. All rights reserved.; Studies implicate a potential role for EAF1 in MLL-ELL induced leukemogenesis; however the biological function of EAF1 in this process remains unknown. In this study, we show that knockdown of zebrafish eaf1 by morpholinos caused serious defects in both primitive and definitive hematopoiesis. Using microarray analysis, we identified foxo3b as a target gene suppressed by eaf1. Ectopic expression of foxo3b in embryos mimicked the phenotypes exhibited in eafl morphants, except that foxo3b had no effect on runxl and c-myb expression while eaf1 morphants did not express these markers in the ventral wall of dorsal aorta. Subsequent experiments showed that a dominant negative form of Foxo3b (dnfoxo3b) partially restored primitive hematopoietic defects in eafl morphants, suggesting that foxo3b might serve as a key factor for mediating eafl function in primitive hematopoiesis. Furthermore, we observed that foxo3b inhibited the transcriptional activity of gata1 and spi1 through protein-protein interaction. Our findings not only suggest a function of eafl on hematopoiesis in vivo, but also reveal a novel regulatory pathway, eaf1-foxo3b-gata1/spi1, that may shed light on the role of EAF1 in MLL-ELL induced leukemogenesis. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordEafl Foxo3b Gata1 Spi1 Hematopoiesis
Department[Hu, Bo; Zhang, Wei; Feng, Xi; Ji, Wei; Xie, Xunwei; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
DOI10.1016/j.ydbio.2014.01.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306]
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000333378200008
WOS KeywordSTEM-CELLS ; ERYTHROID-DIFFERENTIATION ; DEFINITIVE HEMATOPOIESIS ; STEM/PROGENITOR CELLS ; EARLY MACROPHAGES ; C-MYB ; PROMOTER ; PU.1 ; GENE ; ELL
Funding OrganizationNSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306] ; NSFC Grant [91019008, 31071212]; CAS Major Scientific and Technological Project [XDA08010208]; [2010CB126306]
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20089
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorXiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hu, Bo,Zhang, Wei,Feng, Xi,et al. Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis[J]. DEVELOPMENTAL BIOLOGY,2014,388(1):81-93.
APA Hu, Bo.,Zhang, Wei.,Feng, Xi.,Ji, Wei.,Xie, Xunwei.,...&Xiao, WH .(2014).Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis.DEVELOPMENTAL BIOLOGY,388(1),81-93.
MLA Hu, Bo,et al."Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis".DEVELOPMENTAL BIOLOGY 388.1(2014):81-93.
Files in This Item: Download All
File Name/Size DocType Version Access License
Zebrafish eaf1 suppr(15870KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hu, Bo]'s Articles
[Zhang, Wei]'s Articles
[Feng, Xi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hu, Bo]'s Articles
[Zhang, Wei]'s Articles
[Feng, Xi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hu, Bo]'s Articles
[Zhang, Wei]'s Articles
[Feng, Xi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.