IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation
Feng, Lifang1,2; Fu, Chengjie1; Yuan, Dongxia1; Miao, Wei1; Feng, LF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
2014-04-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume149Pages:126-132
AbstractAnalysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved.; Analysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordTetrahymena Thermophila P450 Gene Ddt Degradation
Department[Feng, Lifang; Fu, Chengjie; Yuan, Dongxia; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Feng, Lifang] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China
DOI10.1016/j.aquatox.2014.02.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000334989300014
WOS KeywordSUPPRESSION SUBTRACTIVE HYBRIDIZATION ; INSECTICIDE RESISTANCE ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; EXPRESSED GENES ; TRIBUTYLTIN TBT ; METABOLISM ; CYTOCHROME-P450 ; TRANSFORMATION ; DROSOPHILA ; TOXICITY
Funding OrganizationNational Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20088
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorFeng, LF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,et al. A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation[J]. AQUATIC TOXICOLOGY,2014,149:126-132.
APA Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,Miao, Wei,&Feng, LF .(2014).A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation.AQUATIC TOXICOLOGY,149,126-132.
MLA Feng, Lifang,et al."A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation".AQUATIC TOXICOLOGY 149(2014):126-132.
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