| A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation | |
| Feng, Lifang1,2; Fu, Chengjie1; Yuan, Dongxia1; Miao, Wei1; Feng, LF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. | |
| 2014-04-01 | |
| Source Publication | AQUATIC TOXICOLOGY
 |
| ISSN | 0166-445X |
| Volume | 149Pages:126-132 |
| Abstract | Analysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved.; Analysis of metabolic mechanisms of dichlorodiphenyltrichloroethane (DDT) accumulation and degradation in microorganisms, which could be used to reduce its hazard to higher organisms at the higher in the food chain, have not been investigated. Robust resistance to DDT (grows well in 256 mg/L DDT) and a surprising ability to degrade DDT (more than 70% DDT within 4 h) were found in the ciliated protozoan Tetrahymena thermophila. A P450 gene (CYP5013C2) was found to respond specifically to DDT treatment. In the presence of 256 mg/L DDT, cells with overexpressing CYP5013C2 (p450-OE) grew faster and degraded DDT more efficiently than wild-type (WT) cells, while cells with CYP5013C2 partially knocked down (p450-KD) grew slower and exhibited reduced ability to degrade DDT compared to \NT cells. Both dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DOD) were detected in cells after exposure to DDT, and the concentration of ODD in the p450-OE strain gradually decreased from 0.5 to 4h. Thus, we argue that this P450 gene (CYP5013C2), by efficiently degrading DDT to DDD, is associated with robust resistance to DDT in Tetrahymena, and that a strain overexpressing this gene has the potential to serve as bioreactor that degrades environmental DDT. (C) 2014 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Tetrahymena Thermophila P450 Gene Ddt Degradation |
| Department | [Feng, Lifang; Fu, Chengjie; Yuan, Dongxia; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Feng, Lifang] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China |
| DOI | 10.1016/j.aquatox.2014.02.004 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Toxicology |
| WOS Subject | Marine & Freshwater Biology ; Toxicology |
| WOS ID | WOS:000334989300014 |
| WOS Keyword | SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; INSECTICIDE RESISTANCE ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; EXPRESSED GENES ; TRIBUTYLTIN TBT ; METABOLISM ; CYTOCHROME-P450 ; TRANSFORMATION ; DROSOPHILA ; TOXICITY |
| Funding Organization | National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] ; National Natural Science Foundation of China [30870356]; Scientific Research Foundation for the Introduction of Talent of Zhejiang Gongshang University [1110XJ2110082] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20088 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Feng, LF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China |
| Recommended Citation GB/T 7714 | Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,et al. A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation[J]. AQUATIC TOXICOLOGY,2014,149:126-132. |
| APA | Feng, Lifang,Fu, Chengjie,Yuan, Dongxia,Miao, Wei,&Feng, LF .(2014).A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation.AQUATIC TOXICOLOGY,149,126-132. |
| MLA | Feng, Lifang,et al."A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation".AQUATIC TOXICOLOGY 149(2014):126-132. |
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