| Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis | |
| Ye, Jun1; Chang, Yue2; Yan, Yu1,3; Xiong, Jie2; Xue, Xi-Mei1,3; Yuan, Dongxia2; Sun, Guo-Xin4; Zhu, Yong-Guan1,4; Miao, Wei2; Miao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. | |
| 2014-04-01 | |
| Source Publication | AQUATIC TOXICOLOGY
 |
| ISSN | 0166-445X |
| Volume | 149Pages:50-57 |
| Abstract | Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coil; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.; Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Tetrahymena Pyriformis Protozoan Arsenic Methyltransferase Methylarsine Heavy Metal |
| Department | [Ye, Jun; Yan, Yu; Xue, Xi-Mei; Zhu, Yong-Guan] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China; [Chang, Yue; Xiong, Jie; Yuan, Dongxia; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Yan, Yu; Xue, Xi-Mei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Guo-Xin; Zhu, Yong-Guan] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China |
| DOI | 10.1016/j.aquatox.2014.01.028 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Toxicology |
| WOS Subject | Marine & Freshwater Biology ; Toxicology |
| WOS ID | WOS:000334989300007 |
| WOS Keyword | S-ADENOSYLMETHIONINE METHYLTRANSFERASE ; MARINE FOOD-CHAIN ; TETRAHYMENA-THERMOPHILA ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; SEQUENCE ; BIOTRANSFORMATION ; BIOMETHYLATION ; TRANSFERASE ; METHYLATION |
| Funding Organization | National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20087 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Miao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China |
| Recommended Citation GB/T 7714 | Ye, Jun,Chang, Yue,Yan, Yu,et al. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis[J]. AQUATIC TOXICOLOGY,2014,149:50-57. |
| APA | Ye, Jun.,Chang, Yue.,Yan, Yu.,Xiong, Jie.,Xue, Xi-Mei.,...&Miao, W .(2014).Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis.AQUATIC TOXICOLOGY,149,50-57. |
| MLA | Ye, Jun,et al."Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis".AQUATIC TOXICOLOGY 149(2014):50-57. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Identification and c（1437KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment