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题名: Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis
作者: Ye, Jun1; Chang, Yue2; Yan, Yu1, 3; Xiong, Jie2; Xue, Xi-Mei1, 3; Yuan, Dongxia2; Sun, Guo-Xin4; Zhu, Yong-Guan1, 4; Miao, Wei2
通讯作者: Miao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
关键词: Tetrahymena pyriformis ; Protozoan ; Arsenic ; Methyltransferase ; Methylarsine ; Heavy metal
刊名: AQUATIC TOXICOLOGY
发表日期: 2014-04-01
DOI: 10.1016/j.aquatox.2014.01.028
卷: 149, 页:50-57
收录类别: SCI
文章类型: Article
部门归属: [Ye, Jun; Yan, Yu; Xue, Xi-Mei; Zhu, Yong-Guan] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China; [Chang, Yue; Xiong, Jie; Yuan, Dongxia; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Yan, Yu; Xue, Xi-Mei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Guo-Xin; Zhu, Yong-Guan] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coil; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要: Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: S-ADENOSYLMETHIONINE METHYLTRANSFERASE ; MARINE FOOD-CHAIN ; TETRAHYMENA-THERMOPHILA ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; SEQUENCE ; BIOTRANSFORMATION ; BIOMETHYLATION ; TRANSFERASE ; METHYLATION
语种: 英语
WOS记录号: WOS:000334989300007
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20087
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China

Recommended Citation:
Ye, Jun; Chang, Yue; Yan, Yu; Xiong, Jie; Xue, Xi-Mei; Yuan, Dongxia; Sun, Guo-Xin; Zhu, Yong-Guan; Miao, Wei.Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis,AQUATIC TOXICOLOGY,2014,149():50-57
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