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Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis
Ye, Jun1; Chang, Yue2; Yan, Yu1,3; Xiong, Jie2; Xue, Xi-Mei1,3; Yuan, Dongxia2; Sun, Guo-Xin4; Zhu, Yong-Guan1,4; Miao, Wei2; Miao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
2014-04-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume149Pages:50-57
AbstractArsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coil; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.; Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordTetrahymena Pyriformis Protozoan Arsenic Methyltransferase Methylarsine Heavy Metal
Department[Ye, Jun; Yan, Yu; Xue, Xi-Mei; Zhu, Yong-Guan] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China; [Chang, Yue; Xiong, Jie; Yuan, Dongxia; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Yan, Yu; Xue, Xi-Mei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Guo-Xin; Zhu, Yong-Guan] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
DOI10.1016/j.aquatox.2014.01.028
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000334989300007
WOS KeywordS-ADENOSYLMETHIONINE METHYLTRANSFERASE ; MARINE FOOD-CHAIN ; TETRAHYMENA-THERMOPHILA ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; SEQUENCE ; BIOTRANSFORMATION ; BIOMETHYLATION ; TRANSFERASE ; METHYLATION
Funding OrganizationNational Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] ; National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251]
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20087
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorMiao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Ye, Jun,Chang, Yue,Yan, Yu,et al. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis[J]. AQUATIC TOXICOLOGY,2014,149:50-57.
APA Ye, Jun.,Chang, Yue.,Yan, Yu.,Xiong, Jie.,Xue, Xi-Mei.,...&Miao, W .(2014).Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis.AQUATIC TOXICOLOGY,149,50-57.
MLA Ye, Jun,et al."Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis".AQUATIC TOXICOLOGY 149(2014):50-57.
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