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题名: Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis
作者: Zhong, Liqiao1, 2; Yuan, Li1; Rao, Yu1; Li, Zhouquan1, 2; Zhang, Xiaohua1; Liao, Tao1; Xu, Ying1; Dai, Heping1
通讯作者: Yuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 Southern East Lake Rd, Wuhan 430072, Peoples R China.
关键词: Vitellogenin ; Biomarker ; Zebrafish ; Distribution
刊名: AQUATIC TOXICOLOGY
发表日期: 2014-04-01
DOI: 10.1016/j.aquatox.2014.01.022
卷: 149, 页:1-7
收录类别: SCI
文章类型: Article
部门归属: [Zhong, Liqiao; Yuan, Li; Rao, Yu; Li, Zhouquan; Zhang, Xiaohua; Liao, Tao; Xu, Ying; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhong, Liqiao; Li, Zhouquan] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Vitellogenin (VTG), the precursor of yolk proteins, is a sensitive biomarker of estrogenic contamination in aquatic environments. Traditionally, VTG was believed to be synthesized under the control of estrogen in the livers of mature females and then secreted into the blood, before being taken up by the ovaries and cleaved into lipovitellin and phosvitin, which provide nutrition for developing embryos. However, recent studies have reported that the liver is not the only tissue to express VTG and this has led to questions over the precise tissue distribution of VTG in zebrafish. Moreover, studies in zebrafish on the expression of the VTG protein are rare. Using Western blotting and reverse-transcriptase polymerase chain reaction, this present study reports that the VTG protein and VTG1 mRNA were detected not only in the liver, but also in various extrahepatic tissues, including the heart, spleen, kidney, skin, muscle, gill, eye and brain tissues, of female and 17 alpha-ethinylestradiol (EE2)-treated male zebrafish. Due to the high expression levels of VTG and the ease of taking samples, skin and eye tissues were chosen to evaluate the effects of varying doses and exposure times of EE2 on male zebrafish. The VTG gene and protein were induced by EE2 exposure in liver, skin and eye tissues of male zebrafish in dose- and time-dependent patterns. Therefore, we suggest that zebrafish skin and eye tissues may be alternatives to plasma and liver tissues for VTG biomarker analysis. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要: Vitellogenin (VTG), the precursor of yolk proteins, is a sensitive biomarker of estrogenic contamination in aquatic environments. Traditionally, VTG was believed to be synthesized under the control of estrogen in the livers of mature females and then secreted into the blood, before being taken up by the ovaries and cleaved into lipovitellin and phosvitin, which provide nutrition for developing embryos. However, recent studies have reported that the liver is not the only tissue to express VTG and this has led to questions over the precise tissue distribution of VTG in zebrafish. Moreover, studies in zebrafish on the expression of the VTG protein are rare. Using Western blotting and reverse-transcriptase polymerase chain reaction, this present study reports that the VTG protein and VTG1 mRNA were detected not only in the liver, but also in various extrahepatic tissues, including the heart, spleen, kidney, skin, muscle, gill, eye and brain tissues, of female and 17 alpha-ethinylestradiol (EE2)-treated male zebrafish. Due to the high expression levels of VTG and the ease of taking samples, skin and eye tissues were chosen to evaluate the effects of varying doses and exposure times of EE2 on male zebrafish. The VTG gene and protein were induced by EE2 exposure in liver, skin and eye tissues of male zebrafish in dose- and time-dependent patterns. Therefore, we suggest that zebrafish skin and eye tissues may be alternatives to plasma and liver tissues for VTG biomarker analysis. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: ENDOCRINE-DISRUPTING CHEMICALS ; MINNOW GOBIOCYPRIS-RARUS ; ENVIRONMENTAL ESTROGENS ; EXTRAHEPATIC EXPRESSION ; GENES ; MODEL ; TOXICITY ; EXPOSURE ; 17-BETA-ESTRADIOL ; EVOLUTION
语种: 英语
WOS记录号: WOS:000334989300001
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20086
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Zhong, Liqiao; Yuan, Li; Rao, Yu; Li, Zhouquan; Zhang, Xiaohua; Liao, Tao; Xu, Ying; Dai, Heping.Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis,AQUATIC TOXICOLOGY,2014,149():41646
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