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Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis
Zhong, Liqiao1,2; Yuan, Li1; Rao, Yu1; Li, Zhouquan1,2; Zhang, Xiaohua1; Liao, Tao1; Xu, Ying1; Dai, Heping1; Yuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 Southern East Lake Rd, Wuhan 430072, Peoples R China.
2014-04-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume149Pages:1-7
AbstractVitellogenin (VTG), the precursor of yolk proteins, is a sensitive biomarker of estrogenic contamination in aquatic environments. Traditionally, VTG was believed to be synthesized under the control of estrogen in the livers of mature females and then secreted into the blood, before being taken up by the ovaries and cleaved into lipovitellin and phosvitin, which provide nutrition for developing embryos. However, recent studies have reported that the liver is not the only tissue to express VTG and this has led to questions over the precise tissue distribution of VTG in zebrafish. Moreover, studies in zebrafish on the expression of the VTG protein are rare. Using Western blotting and reverse-transcriptase polymerase chain reaction, this present study reports that the VTG protein and VTG1 mRNA were detected not only in the liver, but also in various extrahepatic tissues, including the heart, spleen, kidney, skin, muscle, gill, eye and brain tissues, of female and 17 alpha-ethinylestradiol (EE2)-treated male zebrafish. Due to the high expression levels of VTG and the ease of taking samples, skin and eye tissues were chosen to evaluate the effects of varying doses and exposure times of EE2 on male zebrafish. The VTG gene and protein were induced by EE2 exposure in liver, skin and eye tissues of male zebrafish in dose- and time-dependent patterns. Therefore, we suggest that zebrafish skin and eye tissues may be alternatives to plasma and liver tissues for VTG biomarker analysis. (C) 2014 Elsevier B.V. All rights reserved.; Vitellogenin (VTG), the precursor of yolk proteins, is a sensitive biomarker of estrogenic contamination in aquatic environments. Traditionally, VTG was believed to be synthesized under the control of estrogen in the livers of mature females and then secreted into the blood, before being taken up by the ovaries and cleaved into lipovitellin and phosvitin, which provide nutrition for developing embryos. However, recent studies have reported that the liver is not the only tissue to express VTG and this has led to questions over the precise tissue distribution of VTG in zebrafish. Moreover, studies in zebrafish on the expression of the VTG protein are rare. Using Western blotting and reverse-transcriptase polymerase chain reaction, this present study reports that the VTG protein and VTG1 mRNA were detected not only in the liver, but also in various extrahepatic tissues, including the heart, spleen, kidney, skin, muscle, gill, eye and brain tissues, of female and 17 alpha-ethinylestradiol (EE2)-treated male zebrafish. Due to the high expression levels of VTG and the ease of taking samples, skin and eye tissues were chosen to evaluate the effects of varying doses and exposure times of EE2 on male zebrafish. The VTG gene and protein were induced by EE2 exposure in liver, skin and eye tissues of male zebrafish in dose- and time-dependent patterns. Therefore, we suggest that zebrafish skin and eye tissues may be alternatives to plasma and liver tissues for VTG biomarker analysis. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordVitellogenin Biomarker Zebrafish Distribution
Department[Zhong, Liqiao; Yuan, Li; Rao, Yu; Li, Zhouquan; Zhang, Xiaohua; Liao, Tao; Xu, Ying; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhong, Liqiao; Li, Zhouquan] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
DOI10.1016/j.aquatox.2014.01.022
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNatural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000334989300001
WOS KeywordENDOCRINE-DISRUPTING CHEMICALS ; MINNOW GOBIOCYPRIS-RARUS ; ENVIRONMENTAL ESTROGENS ; EXTRAHEPATIC EXPRESSION ; GENES ; MODEL ; TOXICITY ; EXPOSURE ; 17-BETA-ESTRADIOL ; EVOLUTION
Funding OrganizationNatural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10] ; Natural Science Foundation of China [21037004, 20777091]; State Key Laboratory of Fresh water Ecology and Biotechnology [2011FBZ10]
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20086
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 Southern East Lake Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Liqiao,Yuan, Li,Rao, Yu,et al. Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis[J]. AQUATIC TOXICOLOGY,2014,149:1-7.
APA Zhong, Liqiao.,Yuan, Li.,Rao, Yu.,Li, Zhouquan.,Zhang, Xiaohua.,...&Yuan, L .(2014).Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis.AQUATIC TOXICOLOGY,149,1-7.
MLA Zhong, Liqiao,et al."Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis".AQUATIC TOXICOLOGY 149(2014):1-7.
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