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题名: Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects
作者: Cheng, Xiaoxia1, 2; Chen, Xiaowen1; Jin, Xia1; He, Jiangyan1; Yin, Zhan1, 3
通讯作者: Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
关键词: gsu alpha promoter ; Pituitary hormone ; Transgenic zebrafish ; gsu alpha expression ; Endocrine disrupting
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2014-07-01
DOI: 10.1016/j.taap.2014.04.009
卷: 278, 期:1, 页:78-84
收录类别: SCI
文章类型: Article
部门归属: [Cheng, Xiaoxia; Chen, Xiaowen; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Cheng, Xiaoxia] Univ Chinese Acad Sci, Beijing, Peoples R China; [Yin, Zhan] State Key Lab Freshwater Ecol & Biotechnol, Ningbo Lab, Ningbo, Zhejiang, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701]
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
摘要: The glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.
英文摘要: The glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.
关键词[WOS]: CRE-MEDIATED RECOMBINATION ; SUBUNIT GENE ; PITUITARY-GLAND ; EFFICIENT TRANSGENESIS ; HORMONE ; TRANSCRIPTION ; EXPRESSION ; RECEPTOR ; ADENOHYPOPHYSIS ; GONADOTROPES
语种: 英语
WOS记录号: WOS:000336887100011
ISSN号: 0041-008X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20082
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.State Key Lab Freshwater Ecol & Biotechnol, Ningbo Lab, Ningbo, Zhejiang, Peoples R China
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