IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects
Cheng, Xiaoxia1,2; Chen, Xiaowen1; Jin, Xia1; He, Jiangyan1; Yin, Zhan1,3; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
2014-07-01
Source PublicationTOXICOLOGY AND APPLIED PHARMACOLOGY
ISSN0041-008X
Volume278Issue:1Pages:78-84
AbstractThe glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.; The glycoprotein subunit alpha(gsu alpha) gene encodes the shared a subunit of the three pituitary heterodimeric glycoprotein hormones: follicle-stimulating hormone beta (Fsh beta), luteinizing hormone beta (Lh beta) and thyroid stimulating hormone beta (Tsh beta). In our current study, we identified and characterized the promoter region of zebrafish gsu alpha and generated a stable gsu alpha:EGFP transgenic line, which recapitulated the endogenous gsu alpha expression in the early developing pituitary gland. A relatively conserved regulatory element set is presented in the promoter regions of zebrafish and three other known mammalian gsu alpha promoters. Our results also demonstrated that the expression patterns of the gsu alpha:EGFP transgene were all identical to those expression patterns of the endogenous gsu alpha expression in the pituitary tissue when our transgenic fish were treated with various endocrine chemicals, including forskolin (FSK), SP600125, trichostatin A (TSA), KCIO4, dexamethasone (Dex),beta-estradiol and progesterone. Thus, this gsu alpha:EGFP transgenic fish reporter line provides another valuable tool for investigating the lineage development of gsu alpha-expressing gonadotrophins and the coordinated regulation of various glycoprotein hormone subunit genes. These reporter fish can serve as a novel platform to perform screenings of endocrine-disrupting chemicals (EDCs) in vivo as well. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordGsu Alpha Promoter Pituitary Hormone Transgenic Zebrafish Gsu Alpha Expression Endocrine Disrupting
Department[Cheng, Xiaoxia; Chen, Xiaowen; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Cheng, Xiaoxia] Univ Chinese Acad Sci, Beijing, Peoples R China; [Yin, Zhan] State Key Lab Freshwater Ecol & Biotechnol, Ningbo Lab, Ningbo, Zhejiang, Peoples R China
DOI10.1016/j.taap.2014.04.009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000336887100011
WOS KeywordCRE-MEDIATED RECOMBINATION ; SUBUNIT GENE ; PITUITARY-GLAND ; EFFICIENT TRANSGENESIS ; HORMONE ; TRANSCRIPTION ; EXPRESSION ; RECEPTOR ; ADENOHYPOPHYSIS ; GONADOTROPES
Funding OrganizationNational Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701] ; National Natural Science Foundation of China [30925027]; National Basic Research Program of China (973 Program) [2010CB126302, 2009CB118701]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20082
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.State Key Lab Freshwater Ecol & Biotechnol, Ningbo Lab, Ningbo, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Xiaoxia,Chen, Xiaowen,Jin, Xia,et al. Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2014,278(1):78-84.
APA Cheng, Xiaoxia,Chen, Xiaowen,Jin, Xia,He, Jiangyan,Yin, Zhan,&Yin, Z .(2014).Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects.TOXICOLOGY AND APPLIED PHARMACOLOGY,278(1),78-84.
MLA Cheng, Xiaoxia,et al."Generation and characterization of gsu alpha:EGFP transgenic zebrafish for evaluating endocrine-disrupting effects".TOXICOLOGY AND APPLIED PHARMACOLOGY 278.1(2014):78-84.
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