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题名: The influence of microgravity on Euglena gracilis as studied on Shenzhou 8
作者: Nasir, A.1; Strauch, S. M.1; Becker, I.1; Sperling, A.1; Schuster, M.1; Richter, P. R.1; Weisskopf, M.1; Ntefidou, M.1; Daiker, V.1; An, Y. A.2; Li, X. Y.2; Liu, Y. D.2; Lebert, M.1
通讯作者: Richter, PR (reprint author), Univ Erlangen Nurnberg, Dept Biol, Dept Cell Biol, D-91054 Erlangen, Germany.
关键词: Euglena gracilis ; gene expression ; microgravity ; qPCR ; space flight ; unicellular flagellate
刊名: PLANT BIOLOGY
发表日期: 2014
DOI: 10.1111/plb.12067
卷: 16, 页:113-119
收录类别: SCI
文章类型: Article
部门归属: [Nasir, A.; Strauch, S. M.; Becker, I.; Sperling, A.; Schuster, M.; Richter, P. R.; Weisskopf, M.; Ntefidou, M.; Daiker, V.; Lebert, M.] Univ Erlangen Nurnberg, Dept Biol, Div Cell Biol, D-91054 Erlangen, Germany; [An, Y. A.; Li, X. Y.; Liu, Y. D.] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: DLR on behalf of the BMBF [50WB1128]
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
摘要: The German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
英文摘要: The German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
关键词[WOS]: RANDOM POSITIONING MACHINE ; GENE-EXPRESSION PATTERNS ; SIMULATED MICROGRAVITY ; ARABIDOPSIS-THALIANA ; INCREASES APOPTOSIS ; SIGNAL-TRANSDUCTION ; ALTERED GRAVITY ; FLAGELLATE ; CELLS ; CYTOSKELETON
语种: 英语
WOS记录号: WOS:000328827200016
ISSN号: 1435-8603
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20074
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Univ Erlangen Nurnberg, Dept Biol, Div Cell Biol, D-91054 Erlangen, Germany
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

Recommended Citation:
Nasir, A.; Strauch, S. M.; Becker, I.; Sperling, A.; Schuster, M.; Richter, P. R.; Weisskopf, M.; Ntefidou, M.; Daiker, V.; An, Y. A.; Li, X. Y.; Liu, Y. D.; Lebert, M..The influence of microgravity on Euglena gracilis as studied on Shenzhou 8,PLANT BIOLOGY,2014,16():113-119
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