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The influence of microgravity on Euglena gracilis as studied on Shenzhou 8
Nasir, A.1; Strauch, S. M.1; Becker, I.1; Sperling, A.1; Schuster, M.1; Richter, P. R.1; Weisskopf, M.1; Ntefidou, M.1; Daiker, V.1; An, Y. A.2; Li, X. Y.2; Liu, Y. D.2; Lebert, M.1; Richter, PR (reprint author), Univ Erlangen Nurnberg, Dept Biol, Dept Cell Biol, D-91054 Erlangen, Germany.
2014
Source PublicationPLANT BIOLOGY
ISSN1435-8603
Volume16Pages:113-119
AbstractThe German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.; The German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
SubtypeArticle
KeywordEuglena Gracilis Gene Expression Microgravity Qpcr Space Flight Unicellular Flagellate
Department[Nasir, A.; Strauch, S. M.; Becker, I.; Sperling, A.; Schuster, M.; Richter, P. R.; Weisskopf, M.; Ntefidou, M.; Daiker, V.; Lebert, M.] Univ Erlangen Nurnberg, Dept Biol, Div Cell Biol, D-91054 Erlangen, Germany; [An, Y. A.; Li, X. Y.; Liu, Y. D.] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1111/plb.12067
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationDLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128]
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000328827200016
WOS KeywordRANDOM POSITIONING MACHINE ; GENE-EXPRESSION PATTERNS ; SIMULATED MICROGRAVITY ; ARABIDOPSIS-THALIANA ; INCREASES APOPTOSIS ; SIGNAL-TRANSDUCTION ; ALTERED GRAVITY ; FLAGELLATE ; CELLS ; CYTOSKELETON
Funding OrganizationDLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128] ; DLR on behalf of the BMBF [50WB1128]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20074
Collection水环境工程研究中心_期刊论文
Corresponding AuthorRichter, PR (reprint author), Univ Erlangen Nurnberg, Dept Biol, Dept Cell Biol, D-91054 Erlangen, Germany.
Affiliation1.Univ Erlangen Nurnberg, Dept Biol, Div Cell Biol, D-91054 Erlangen, Germany
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Nasir, A.,Strauch, S. M.,Becker, I.,et al. The influence of microgravity on Euglena gracilis as studied on Shenzhou 8[J]. PLANT BIOLOGY,2014,16:113-119.
APA Nasir, A..,Strauch, S. M..,Becker, I..,Sperling, A..,Schuster, M..,...&Richter, PR .(2014).The influence of microgravity on Euglena gracilis as studied on Shenzhou 8.PLANT BIOLOGY,16,113-119.
MLA Nasir, A.,et al."The influence of microgravity on Euglena gracilis as studied on Shenzhou 8".PLANT BIOLOGY 16(2014):113-119.
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