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Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate
Han, Zhihua1,2; Wang, Qiangwei3; Fu, Jie1,2; Chen, Hongshan4,5; Zhao, Ye5; Zhou, Bingsheng3; Gong, Zhiyuan5; Wei, Si1,2; Li, Jun1,2; Liu, Hongling1,2; Zhang, Xiaowei1,2; Liu, Chunsheng1,2,5,6; Yu, Hongxia1,2; Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China.
2014-05-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume150Issue:-Pages:175-181
AbstractThe flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved.; The flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordDevelopmental Toxicity Zebrafish Itraq Flame Retardants Tbep
Department[Han, Zhihua; Fu, Jie; Wei, Si; Li, Jun; Liu, Hongling; Zhang, Xiaowei; Liu, Chunsheng; Yu, Hongxia] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China; [Han, Zhihua; Fu, Jie; Wei, Si; Li, Jun; Liu, Hongling; Zhang, Xiaowei; Liu, Chunsheng; Yu, Hongxia] Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China; [Wang, Qiangwei; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Hongshan] NE Normal Univ, Sch Environm, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130024, Peoples R China; [Chen, Hongshan; Zhao, Ye; Gong, Zhiyuan; Liu, Chunsheng] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore; [Liu, Chunsheng] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1016/j.aquatox.2014.03.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000336187900020
WOS KeywordORGANOPHOSPHORUS FLAME RETARDANTS ; MESSENGER-RNA EXPRESSION ; TRIS(1,3-DICHLORO-2-PROPYL) PHOSPHATE ; MASS-SPECTROMETRY ; XENOPUS-LAEVIS ; PLASTICIZERS ; PROTEINS ; FISH ; CHROMATOGRAPHY ; TRANSCRIPTION
Funding OrganizationNational Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] ; National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044]
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20065
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China.
Affiliation1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.NE Normal Univ, Sch Environm, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130024, Peoples R China
5.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
6.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Han, Zhihua,Wang, Qiangwei,Fu, Jie,et al. Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate[J]. AQUATIC TOXICOLOGY,2014,150(-):175-181.
APA Han, Zhihua.,Wang, Qiangwei.,Fu, Jie.,Chen, Hongshan.,Zhao, Ye.,...&Liu, CS .(2014).Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate.AQUATIC TOXICOLOGY,150(-),175-181.
MLA Han, Zhihua,et al."Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate".AQUATIC TOXICOLOGY 150.-(2014):175-181.
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