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题名: Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate
作者: Han, Zhihua1, 2; Wang, Qiangwei3; Fu, Jie1, 2; Chen, Hongshan4, 5; Zhao, Ye5; Zhou, Bingsheng3; Gong, Zhiyuan5; Wei, Si1, 2; Li, Jun1, 2; Liu, Hongling1, 2; Zhang, Xiaowei1, 2; Liu, Chunsheng1, 2, 5, 6; Yu, Hongxia1, 2
通讯作者: Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China.
关键词: Developmental toxicity ; Zebrafish ; iTRAQ ; Flame retardants ; TBEP
刊名: AQUATIC TOXICOLOGY
发表日期: 2014-05-01
DOI: 10.1016/j.aquatox.2014.03.013
卷: 150, 期:-, 页:175-181
收录类别: SCI
文章类型: Article
部门归属: [Han, Zhihua; Fu, Jie; Wei, Si; Li, Jun; Liu, Hongling; Zhang, Xiaowei; Liu, Chunsheng; Yu, Hongxia] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China; [Han, Zhihua; Fu, Jie; Wei, Si; Li, Jun; Liu, Hongling; Zhang, Xiaowei; Liu, Chunsheng; Yu, Hongxia] Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China; [Wang, Qiangwei; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Hongshan] NE Normal Univ, Sch Environm, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130024, Peoples R China; [Chen, Hongshan; Zhao, Ye; Gong, Zhiyuan; Liu, Chunsheng] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore; [Liu, Chunsheng] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: The flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要: The flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: ORGANOPHOSPHORUS FLAME RETARDANTS ; MESSENGER-RNA EXPRESSION ; TRIS(1,3-DICHLORO-2-PROPYL) PHOSPHATE ; MASS-SPECTROMETRY ; XENOPUS-LAEVIS ; PLASTICIZERS ; PROTEINS ; FISH ; CHROMATOGRAPHY ; TRANSCRIPTION
语种: 英语
WOS记录号: WOS:000336187900020
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20065
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.NE Normal Univ, Sch Environm, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130024, Peoples R China
5.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
6.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
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