IHB OpenIR  > 水环境工程研究中心  > 期刊论文
ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71
Yu, Liqin1,2; Liu, Chunsheng1; Chen, Qi1; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2014-06-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume33Issue:6Pages:1354-1362
AbstractThe objective of the present study was to investigate the impact of polybrominated diphenyl ethers (PBDEs) on fish reproduction over 2 generations. Zebrafish (Danio rerio) embryos (F0) were exposed to low concentrations (3 mu g/L, 10 mu g/L, and 30 mu g/L) of the PBDE mixture DE-71 until they were sexually mature, and steroid hormone production, expression of genes involved in steroidogenesis, gonadal development, and gamete characteristics were examined. Exposure of female zebrafish to DE-71 resulted in lower estradiol production and downregulation of cytochrome P450 aromatase mRNA. In males, exposure to DE-71 resulted in greater testosterone production and greater cytochrome P450 c17 -hydroxylase,17,20-lase mRNA expression. Moreover, hepatic vitellogenin mRNA and estrogenic receptor gene transcription were downregulated in females and males. Expression of the follicle-stimulating hormone gene in the pituitary was upregulated, and the expression of luteinizing hormone was downregulated in both sexes. Histological examination showed inhibition of oocyte maturation in females and retarded spermiation in males. The average number of eggs (F1) produced was also reduced. Additionally, exposure of F0 embryos to DE-71 did not result in developmental toxicity, whereas delayed hatching, reduced survival, and decreased growth were observed in the F1 embryos derived from parent fish exposed to DE-71. Therefore, long-term exposure to low concentrations of PBDEs in zebrafish could cause reproductive impairment, suggesting that PBDEs might have significant adverse effects on fish population in the highly PBDEs-contaminated aquatic environment. Environ Toxicol Chem 2014;33:1354-1362. (c) 2014 SETAC; The objective of the present study was to investigate the impact of polybrominated diphenyl ethers (PBDEs) on fish reproduction over 2 generations. Zebrafish (Danio rerio) embryos (F0) were exposed to low concentrations (3 mu g/L, 10 mu g/L, and 30 mu g/L) of the PBDE mixture DE-71 until they were sexually mature, and steroid hormone production, expression of genes involved in steroidogenesis, gonadal development, and gamete characteristics were examined. Exposure of female zebrafish to DE-71 resulted in lower estradiol production and downregulation of cytochrome P450 aromatase mRNA. In males, exposure to DE-71 resulted in greater testosterone production and greater cytochrome P450 c17 -hydroxylase,17,20-lase mRNA expression. Moreover, hepatic vitellogenin mRNA and estrogenic receptor gene transcription were downregulated in females and males. Expression of the follicle-stimulating hormone gene in the pituitary was upregulated, and the expression of luteinizing hormone was downregulated in both sexes. Histological examination showed inhibition of oocyte maturation in females and retarded spermiation in males. The average number of eggs (F1) produced was also reduced. Additionally, exposure of F0 embryos to DE-71 did not result in developmental toxicity, whereas delayed hatching, reduced survival, and decreased growth were observed in the F1 embryos derived from parent fish exposed to DE-71. Therefore, long-term exposure to low concentrations of PBDEs in zebrafish could cause reproductive impairment, suggesting that PBDEs might have significant adverse effects on fish population in the highly PBDEs-contaminated aquatic environment. Environ Toxicol Chem 2014;33:1354-1362. (c) 2014 SETAC
SubtypeArticle
KeywordPolybrominated Diphenyl Ethers (Pbdes) Reproductive Toxicity Endocrine Disruption Sex Hormone Zebrafish
Department[Yu, Liqin; Liu, Chunsheng; Chen, Qi; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Yu, Liqin] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
DOI10.1002/etc.2562
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000335936900005
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; H295R CELL-LINE ; MINNOW PIMEPHALES-PROMELAS ; KESTRELS FALCO-SPARVERIUS ; FATHEAD MINNOW ; DANIO-RERIO ; AMERICAN KESTRELS ; GENE-EXPRESSION ; IN-VIVO ; DEVELOPMENTAL NEUROTOXICITY
Funding OrganizationNational Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20062
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Yu, Liqin,Liu, Chunsheng,Chen, Qi,et al. ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2014,33(6):1354-1362.
APA Yu, Liqin,Liu, Chunsheng,Chen, Qi,Zhou, Bingsheng,&Zhou, BS .(2014).ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,33(6),1354-1362.
MLA Yu, Liqin,et al."ENDOCRINE DISRUPTION AND REPRODUCTION IMPAIRMENT IN ZEBRAFISH AFTER LONG-TERM EXPOSURE TO DE-71".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 33.6(2014):1354-1362.
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