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Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants
Zhu, Rong; Chen, Zhong-yuan; Wang, Jun; Yuan, Jiang-di; Liao, Xiang-yong; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2014
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume42Issue:2Pages:311-322
AbstractA series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved.; A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMhc Of Chinese Giant Salamanders Andrias Davidianus (Anda-mhc) Genetic Diversity Alternative Splice Variant Andrias Davidianus Ranavirus (Adrv) Inducible Expression
Department[Zhu, Rong ; Chen, Zhong-yuan ; Wang, Jun ; Yuan, Jiang-di ; Liao, Xiang-yong ; Gui, Jian-Fang ; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.dci.2013.10.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000328725800021
WOS KeywordMAJOR HISTOCOMPATIBILITY COMPLEX ; WILD TIGER SALAMANDERS ; CLASS-I GENES ; MOLECULAR CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SELECTION ; POLYMORPHISM ; MECHANISMS ; EVOLUTION ; RANAVIRUS
Funding OrganizationNational Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239]
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20059
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Rong,Chen, Zhong-yuan,Wang, Jun,et al. Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,42(2):311-322.
APA Zhu, Rong.,Chen, Zhong-yuan.,Wang, Jun.,Yuan, Jiang-di.,Liao, Xiang-yong.,...&Zhang, QY .(2014).Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,42(2),311-322.
MLA Zhu, Rong,et al."Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 42.2(2014):311-322.
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