| Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants | |
| Zhu, Rong; Chen, Zhong-yuan; Wang, Jun; Yuan, Jiang-di; Liao, Xiang-yong; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2014 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| Volume | 42Issue:2Pages:311-322 |
| Abstract | A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved.; A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Mhc Of Chinese Giant Salamanders Andrias Davidianus (Anda-mhc) Genetic Diversity Alternative Splice Variant Andrias Davidianus Ranavirus (Adrv) Inducible Expression |
| Department | [Zhu, Rong ; Chen, Zhong-yuan ; Wang, Jun ; Yuan, Jiang-di ; Liao, Xiang-yong ; Gui, Jian-Fang ; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1016/j.dci.2013.10.001 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Immunology ; Zoology |
| WOS Subject | Immunology ; Zoology |
| WOS ID | WOS:000328725800021 |
| WOS Keyword | MAJOR HISTOCOMPATIBILITY COMPLEX ; WILD TIGER SALAMANDERS ; CLASS-I GENES ; MOLECULAR CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SELECTION ; POLYMORPHISM ; MECHANISMS ; EVOLUTION ; RANAVIRUS |
| Funding Organization | National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] ; National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20059 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Rong,Chen, Zhong-yuan,Wang, Jun,et al. Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,42(2):311-322. |
| APA | Zhu, Rong.,Chen, Zhong-yuan.,Wang, Jun.,Yuan, Jiang-di.,Liao, Xiang-yong.,...&Zhang, QY .(2014).Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,42(2),311-322. |
| MLA | Zhu, Rong,et al."Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 42.2(2014):311-322. |
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