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题名: Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata
作者: Hu, Bao-Qing1; Liu, Yi2; Wen, Chun-Gen1; Li, Ai-Hua3; Hu, Xiang-Ping1; Wu, Dan1; Hu, Xiao-Juan1; Tao, Zhi-Ying1
通讯作者: Hu, BQ (reprint author), Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China.
关键词: Cristaria plicata ; Selenoprotein W (SelW) ; Gene cloning ; mRNA expression
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2014
DOI: 10.1016/j.cbpb.2013.09.008
卷: 167, 页:8-15
收录类别: SCI
文章类型: Article
部门归属: [Hu, Bao-Qing ; Wen, Chun-Gen ; Hu, Xiang-Ping ; Wu, Dan ; Hu, Xiao-Juan ; Tao, Zhi-Ying] Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China ; [Liu, Yi] Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China ; [Li, Ai-Hua] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024]
类目[WOS]: Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Zoology
摘要: Selenoprotein W (SelW) is a selenocysteine containing protein with redox activity involved in the antioxidant response. In this study, a selenoprotein W was cloned from pearl mussel Cristaria plicata (designated as CpSelW), and the expression patterns were characterized in tissues after Aeromonas hydrophila challenged. The full-length cDNA of cpSelW was of 858 bp, containing a 5' untranslated region (UTR) of 145 bp, a 3' UTR of 455 bp with a poly (A) tail, and an open reading frame (ORF) of 258 bp encoding a polypeptide of 85 amino acids with the predicted molecular mass of 9277 kDa, which shared 61% identity with SelW from Gallus gallus. A tertiary structure model generated for the CpSelW displayed a beta-alpha-beta-beta-beta-alpha secondary structure pattern, which was similar to mouse SelW protein 3D structure. The mRNA of CpSelW was constitutively expressed in tested tissues of healthy mussel, including mantle, gill, hemocytes, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. After mussels were stimulated by A. hydrophila, the mRNA expression of CpSelW in hemocytes at 6, 12 and 24 h, in gill at 12 h and in hepatopancreas at 24 h was significantly down-regulated. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: Selenoprotein W (SelW) is a selenocysteine containing protein with redox activity involved in the antioxidant response. In this study, a selenoprotein W was cloned from pearl mussel Cristaria plicata (designated as CpSelW), and the expression patterns were characterized in tissues after Aeromonas hydrophila challenged. The full-length cDNA of cpSelW was of 858 bp, containing a 5' untranslated region (UTR) of 145 bp, a 3' UTR of 455 bp with a poly (A) tail, and an open reading frame (ORF) of 258 bp encoding a polypeptide of 85 amino acids with the predicted molecular mass of 9277 kDa, which shared 61% identity with SelW from Gallus gallus. A tertiary structure model generated for the CpSelW displayed a beta-alpha-beta-beta-beta-alpha secondary structure pattern, which was similar to mouse SelW protein 3D structure. The mRNA of CpSelW was constitutively expressed in tested tissues of healthy mussel, including mantle, gill, hemocytes, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. After mussels were stimulated by A. hydrophila, the mRNA expression of CpSelW in hemocytes at 6, 12 and 24 h, in gill at 12 h and in hepatopancreas at 24 h was significantly down-regulated. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: DEPENDENT GLUTATHIONE-PEROXIDASE ; SELENOCYSTEINE INCORPORATION ; OXIDATIVE STRESS ; GENE-EXPRESSION ; SECIS ELEMENTS ; IN-VIVO ; SELENIUM ; PROTEIN ; IDENTIFICATION ; EUKARYOTES
语种: 英语
WOS记录号: WOS:000329019800002
ISSN号: 1096-4959
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20057
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China
2.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China

Recommended Citation:
Hu, Bao-Qing; Liu, Yi; Wen, Chun-Gen; Li, Ai-Hua; Hu, Xiang-Ping; Wu, Dan; Hu, Xiao-Juan; Tao, Zhi-Ying.Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,167():41866
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