IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata
Hu, Bao-Qing1; Liu, Yi2; Wen, Chun-Gen1; Li, Ai-Hua3; Hu, Xiang-Ping1; Wu, Dan1; Hu, Xiao-Juan1; Tao, Zhi-Ying1; Hu, BQ (reprint author), Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China.
2014
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume167Pages:8-15
AbstractSelenoprotein W (SelW) is a selenocysteine containing protein with redox activity involved in the antioxidant response. In this study, a selenoprotein W was cloned from pearl mussel Cristaria plicata (designated as CpSelW), and the expression patterns were characterized in tissues after Aeromonas hydrophila challenged. The full-length cDNA of cpSelW was of 858 bp, containing a 5' untranslated region (UTR) of 145 bp, a 3' UTR of 455 bp with a poly (A) tail, and an open reading frame (ORF) of 258 bp encoding a polypeptide of 85 amino acids with the predicted molecular mass of 9277 kDa, which shared 61% identity with SelW from Gallus gallus. A tertiary structure model generated for the CpSelW displayed a beta-alpha-beta-beta-beta-alpha secondary structure pattern, which was similar to mouse SelW protein 3D structure. The mRNA of CpSelW was constitutively expressed in tested tissues of healthy mussel, including mantle, gill, hemocytes, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. After mussels were stimulated by A. hydrophila, the mRNA expression of CpSelW in hemocytes at 6, 12 and 24 h, in gill at 12 h and in hepatopancreas at 24 h was significantly down-regulated. (C) 2013 Elsevier Inc. All rights reserved.; Selenoprotein W (SelW) is a selenocysteine containing protein with redox activity involved in the antioxidant response. In this study, a selenoprotein W was cloned from pearl mussel Cristaria plicata (designated as CpSelW), and the expression patterns were characterized in tissues after Aeromonas hydrophila challenged. The full-length cDNA of cpSelW was of 858 bp, containing a 5' untranslated region (UTR) of 145 bp, a 3' UTR of 455 bp with a poly (A) tail, and an open reading frame (ORF) of 258 bp encoding a polypeptide of 85 amino acids with the predicted molecular mass of 9277 kDa, which shared 61% identity with SelW from Gallus gallus. A tertiary structure model generated for the CpSelW displayed a beta-alpha-beta-beta-beta-alpha secondary structure pattern, which was similar to mouse SelW protein 3D structure. The mRNA of CpSelW was constitutively expressed in tested tissues of healthy mussel, including mantle, gill, hemocytes, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. After mussels were stimulated by A. hydrophila, the mRNA expression of CpSelW in hemocytes at 6, 12 and 24 h, in gill at 12 h and in hepatopancreas at 24 h was significantly down-regulated. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordCristaria Plicata Selenoprotein w (Selw) Gene Cloning Mrna Expression
Department[Hu, Bao-Qing ; Wen, Chun-Gen ; Hu, Xiang-Ping ; Wu, Dan ; Hu, Xiao-Juan ; Tao, Zhi-Ying] Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China ; [Liu, Yi] Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China ; [Li, Ai-Hua] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
DOI10.1016/j.cbpb.2013.09.008
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000329019800002
WOS KeywordDEPENDENT GLUTATHIONE-PEROXIDASE ; SELENOCYSTEINE INCORPORATION ; OXIDATIVE STRESS ; GENE-EXPRESSION ; SECIS ELEMENTS ; IN-VIVO ; SELENIUM ; PROTEIN ; IDENTIFICATION ; EUKARYOTES
Funding OrganizationNational Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024] ; National Natural Science Foundation of China [30960296, 31060359]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2013FB09]; Key Scientific and Technological Programme of Nanchang [2012-KJ2C-NY-SC-002]; Scientific and Technological of Education Department of Jiangxi Province, China [GJJ10378, GJJ12024]
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20057
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, BQ (reprint author), Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China.
Affiliation1.Nanchang Univ, Inst Life Sci, Sch Life Sci & Food Engn, Nanchang 330031, Peoples R China
2.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Hu, Bao-Qing,Liu, Yi,Wen, Chun-Gen,et al. Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,167:8-15.
APA Hu, Bao-Qing.,Liu, Yi.,Wen, Chun-Gen.,Li, Ai-Hua.,Hu, Xiang-Ping.,...&Hu, BQ .(2014).Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,167,8-15.
MLA Hu, Bao-Qing,et al."Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 167(2014):8-15.
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