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C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus
Mei, Jie1; Yue, Hua-Mei2; Li, Zhi2; Chen, Bo2; Zhong, Jian-Xiang2; Dan, Cheng2; Zhou, Li2; Gui, Jian-Fang1,2; Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
2014
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume10Issue:1Pages:15-24
AbstractC1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.; C1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.
SubtypeArticle
KeywordC1q-like Microrna Mir-430 Knockdown Primordial Germ Cell Early Embryogenesis
Department[Mei, Jie ; Gui, Jian-Fang] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China ; [Yue, Hua-Mei ; Li, Zhi ; Chen, Bo ; Zhong, Jian-Xiang ; Dan, Cheng ; Zhou, Li ; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.7150/ijbs.7490
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000329338400002
WOS KeywordC1Q FAMILY-MEMBER ; MESSENGER-RNAS ; GIBEL CARP ; ZEBRAFISH MIR-430 ; STEM-CELLS ; EXPRESSION ; IDENTIFICATION ; PROLIFERATION ; MIGRATION ; GUIDANCE
Funding OrganizationNational Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931] ; National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931]
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20056
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorMei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Mei, Jie,Yue, Hua-Mei,Li, Zhi,et al. C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2014,10(1):15-24.
APA Mei, Jie.,Yue, Hua-Mei.,Li, Zhi.,Chen, Bo.,Zhong, Jian-Xiang.,...&Mei, J .(2014).C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,10(1),15-24.
MLA Mei, Jie,et al."C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 10.1(2014):15-24.
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