中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus
作者: Mei, Jie1; Yue, Hua-Mei2; Li, Zhi2; Chen, Bo2; Zhong, Jian-Xiang2; Dan, Cheng2; Zhou, Li2; Gui, Jian-Fang1, 2
通讯作者: Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
关键词: C1q-like ; microRNA ; miR-430 ; knockdown ; primordial germ cell ; early embryogenesis
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
发表日期: 2014
DOI: 10.7150/ijbs.7490
卷: 10, 期:1, 页:15-24
收录类别: SCI
文章类型: Article
部门归属: [Mei, Jie ; Gui, Jian-Fang] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China ; [Yue, Hua-Mei ; Li, Zhi ; Chen, Bo ; Zhong, Jian-Xiang ; Dan, Cheng ; Zhou, Li ; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931]
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
摘要: C1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.
英文摘要: C1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.
关键词[WOS]: C1Q FAMILY-MEMBER ; MESSENGER-RNAS ; GIBEL CARP ; ZEBRAFISH MIR-430 ; STEM-CELLS ; EXPRESSION ; IDENTIFICATION ; PROLIFERATION ; MIGRATION ; GUIDANCE
语种: 英语
WOS记录号: WOS:000329338400002
ISSN号: 1449-2288
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20056
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus.pdf(1013KB)----开放获取View 联系获取全文

作者单位: 1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Mei, Jie; Yue, Hua-Mei; Li, Zhi; Chen, Bo; Zhong, Jian-Xiang; Dan, Cheng; Zhou, Li; Gui, Jian-Fang.C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus,INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2014,10(1):15-24
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Mei, Jie]'s Articles
[Yue, Hua-Mei]'s Articles
[Li, Zhi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Mei, Jie]‘s Articles
[Yue, Hua-Mei]‘s Articles
[Li, Zhi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace