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题名: Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.
作者: Cao, Mengxi1, 2; Chen, Ji1; Peng, Wei1, 2; Wang, Yaping1; Liao, Lanjie1; Li, Yongming1; Trudeau, Vance L.3; Zhu, Zuoyan1; Hu, Wei1
通讯作者: Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Growth hormone ; Reproduction ; Interaction ; Luteinizing hormone ; GH-transgenic common carp
刊名: GENERAL AND COMPARATIVE ENDOCRINOLOGY
发表日期: 2014
DOI: 10.1016/j.ygcen.2013.10.011
卷: 195, 页:47-57
收录类别: SCI
文章类型: Article
部门归属: [Cao, Mengxi ; Chen, Ji ; Peng, Wei ; Wang, Yaping ; Liao, Lanjie ; Li, Yongming ; Zhu, Zuoyan ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Cao, Mengxi ; Peng, Wei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Trudeau, Vance L.] Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
摘要: To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: GONADOTROPIN-RELEASING-HORMONE ; TRANSGENIC COHO SALMON ; TILAPIA OREOCHROMIS-NILOTICUS ; SUBUNIT GENE-EXPRESSION ; GOLDFISH CARASSIUS-AURATUS ; MESSENGER-RNA ; ONCORHYNCHUS-KISUTCH ; ATLANTIC SALMON ; FEEDBACK LOOP ; JAPANESE EEL
语种: 英语
WOS记录号: WOS:000330257100006
ISSN号: 0016-6480
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20055
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada

Recommended Citation:
Cao, Mengxi; Chen, Ji; Peng, Wei; Wang, Yaping; Liao, Lanjie; Li, Yongming; Trudeau, Vance L.; Zhu, Zuoyan; Hu, Wei.Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.,GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,195():47-57
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