| Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L. | |
| Cao, Mengxi1,2; Chen, Ji1; Peng, Wei1,2; Wang, Yaping1; Liao, Lanjie1; Li, Yongming1; Trudeau, Vance L.3; Zhu, Zuoyan1; Hu, Wei1; Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2014 | |
| Source Publication | GENERAL AND COMPARATIVE ENDOCRINOLOGY
 |
| ISSN | 0016-6480 |
| Volume | 195Pages:47-57 |
| Abstract | To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.; To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved. |
| Subtype | Article |
| Keyword | Growth Hormone Reproduction Interaction Luteinizing Hormone Gh-transgenic Common Carp |
| Department | [Cao, Mengxi ; Chen, Ji ; Peng, Wei ; Wang, Yaping ; Liao, Lanjie ; Li, Yongming ; Zhu, Zuoyan ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Cao, Mengxi ; Peng, Wei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Trudeau, Vance L.] Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada |
| DOI | 10.1016/j.ygcen.2013.10.011 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Endocrinology & Metabolism |
| WOS Subject | Endocrinology & Metabolism |
| WOS ID | WOS:000330257100006 |
| WOS Keyword | GONADOTROPIN-RELEASING-HORMONE ; TRANSGENIC COHO SALMON ; TILAPIA OREOCHROMIS-NILOTICUS ; SUBUNIT GENE-EXPRESSION ; GOLDFISH CARASSIUS-AURATUS ; MESSENGER-RNA ; ONCORHYNCHUS-KISUTCH ; ATLANTIC SALMON ; FEEDBACK LOOP ; JAPANESE EEL |
| Funding Organization | 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20055 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada |
| Recommended Citation GB/T 7714 | Cao, Mengxi,Chen, Ji,Peng, Wei,et al. Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,195:47-57. |
| APA | Cao, Mengxi.,Chen, Ji.,Peng, Wei.,Wang, Yaping.,Liao, Lanjie.,...&Hu, W .(2014).Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L..GENERAL AND COMPARATIVE ENDOCRINOLOGY,195,47-57. |
| MLA | Cao, Mengxi,et al."Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.".GENERAL AND COMPARATIVE ENDOCRINOLOGY 195(2014):47-57. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Effects of growth ho（3382KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment