IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.
Cao, Mengxi1,2; Chen, Ji1; Peng, Wei1,2; Wang, Yaping1; Liao, Lanjie1; Li, Yongming1; Trudeau, Vance L.3; Zhu, Zuoyan1; Hu, Wei1; Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2014
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
Volume195Pages:47-57
AbstractTo study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.; To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordGrowth Hormone Reproduction Interaction Luteinizing Hormone Gh-transgenic Common Carp
Department[Cao, Mengxi ; Chen, Ji ; Peng, Wei ; Wang, Yaping ; Liao, Lanjie ; Li, Yongming ; Zhu, Zuoyan ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Cao, Mengxi ; Peng, Wei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Trudeau, Vance L.] Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada
DOI10.1016/j.ygcen.2013.10.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000330257100006
WOS KeywordGONADOTROPIN-RELEASING-HORMONE ; TRANSGENIC COHO SALMON ; TILAPIA OREOCHROMIS-NILOTICUS ; SUBUNIT GENE-EXPRESSION ; GOLDFISH CARASSIUS-AURATUS ; MESSENGER-RNA ; ONCORHYNCHUS-KISUTCH ; ATLANTIC SALMON ; FEEDBACK LOOP ; JAPANESE EEL
Funding Organization863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program ; 863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20055
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada
Recommended Citation
GB/T 7714
Cao, Mengxi,Chen, Ji,Peng, Wei,et al. Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,195:47-57.
APA Cao, Mengxi.,Chen, Ji.,Peng, Wei.,Wang, Yaping.,Liao, Lanjie.,...&Hu, W .(2014).Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L..GENERAL AND COMPARATIVE ENDOCRINOLOGY,195,47-57.
MLA Cao, Mengxi,et al."Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.".GENERAL AND COMPARATIVE ENDOCRINOLOGY 195(2014):47-57.
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