IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant
Zou, Peng Fei1,2,3; Chang, Ming Xian1; Xue, Na Na1; Liu, Xue Qin4; Li, Jun Hua1; Fu, Jian Ping1; Chen, Shan Nan1; Nie, Pin1,3; Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
2014-02-01
Source PublicationIMMUNOLOGY
ISSN0019-2805
Volume141Issue:2Pages:192-202
AbstractMelanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.; Melanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.
SubtypeArticle
KeywordMelanoma Differentiation-associated Gene 5 Mitochondrial Antiviral Signalling Protein Splicing Variant Type i Interferon Zebrafish
Department[Zou, Peng Fei; Chang, Ming Xian; Xue, Na Na; Li, Jun Hua; Fu, Jian Ping; Chen, Shan Nan; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China; [Zou, Peng Fei] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China; [Zou, Peng Fei; Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China; [Liu, Xue Qin] Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
DOI10.1111/imm.12179
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationBasic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083]
Indexed BySCI
Language英语
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000329459000007
WOS KeywordI-LIKE RECEPTORS ; RIG-I ; EDWARDSIELLA-TARDA ; RNA HELICASE ; PATHOGEN RECOGNITION ; INNATE IMMUNITY ; MDA5 ; VIRUS ; ACTIVATION ; EXPRESSION
Funding OrganizationBasic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083] ; Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083]
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20052
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zou, Peng Fei,Chang, Ming Xian,Xue, Na Na,et al. Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant[J]. IMMUNOLOGY,2014,141(2):192-202.
APA Zou, Peng Fei.,Chang, Ming Xian.,Xue, Na Na.,Liu, Xue Qin.,Li, Jun Hua.,...&Chang, MX .(2014).Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant.IMMUNOLOGY,141(2),192-202.
MLA Zou, Peng Fei,et al."Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant".IMMUNOLOGY 141.2(2014):192-202.
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