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题名: Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant
作者: Zou, Peng Fei1, 2, 3; Chang, Ming Xian1; Xue, Na Na1; Liu, Xue Qin4; Li, Jun Hua1; Fu, Jian Ping1; Chen, Shan Nan1; Nie, Pin1, 3
通讯作者: Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
关键词: melanoma differentiation-associated gene 5 ; mitochondrial antiviral signalling protein ; splicing variant ; type I interferon ; zebrafish
刊名: IMMUNOLOGY
发表日期: 2014-02-01
DOI: 10.1111/imm.12179
卷: 141, 期:2, 页:192-202
收录类别: SCI
文章类型: Article
部门归属: [Zou, Peng Fei; Chang, Ming Xian; Xue, Na Na; Li, Jun Hua; Fu, Jian Ping; Chen, Shan Nan; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China; [Zou, Peng Fei] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China; [Zou, Peng Fei; Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China; [Liu, Xue Qin] Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083]
类目[WOS]: Immunology
研究领域[WOS]: Immunology
摘要: Melanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.
英文摘要: Melanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.
关键词[WOS]: I-LIKE RECEPTORS ; RIG-I ; EDWARDSIELLA-TARDA ; RNA HELICASE ; PATHOGEN RECOGNITION ; INNATE IMMUNITY ; MDA5 ; VIRUS ; ACTIVATION ; EXPRESSION
语种: 英语
WOS记录号: WOS:000329459000007
ISSN号: 0019-2805
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20052
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
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