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Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare
Tian, J. Y.1; Qi, Z. T.2; Wu, N.3; Chang, M. X.4; Nie, P.4; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2014-02-01
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume37Issue:2Pages:89-101
AbstractIn this study, the constant-region genes (C alpha, C beta and C gamma) that encode the T-cell antigen receptor (TCR) alpha, beta and gamma chains were cloned from mandarin fish, Siniperca chuatsi Basilewsky, an important freshwater fish species in China. The complementary DNA sequences of C alpha, C beta and C gamma were 843, 716 and 906base pairs (bp) in length and had a 465-, 289- and 360-bp 3 ' untranslated region, encoding 125, 142 and 182 amino acids, respectively. The amino-acid sequences of the constant regions of mandarin fish TCR alpha, beta and gamma chains (encoded by C alpha, C beta and C gamma, respectively) were most similar to those of their teleost counterparts, showing 60% similarity with pufferfish, 48% similarity with Atlantic salmon and 57% similarity with flounder, respectively. The phylogenetic analysis revealed that the mandarin fish C alpha, C beta and C gamma were clustered, respectively, with their vertebrate counterparts. The mandarin fish C alpha, C beta and C gamma could also be separated into four domains: immunoglobulin; connecting peptide (CP); transmembrane (TM); and cytoplasmic tail. Several conserved features in mammalian TCRs were also found in those of mandarin fish, such as a conserved cysteine residue in the CP domain of C alpha, necessary for creating an interchain disulphide bond with the TCR beta chain, and a conserved antigen receptor TM motif in C alpha and C beta. Meanwhile, transcripts of C alpha, C beta and C gamma were detectable in all examined organs, with a stronger signal observed in lymphoid organs. In addition, the temporal transcriptional changes for C alpha and C gamma were investigated, 1, 2, 3, 4, 5, 6 and 8weeks after stimulation with Flavobacterium columnare, in head kidney, spleen, blood, thymus, gill and intestine, using real-time polymerase chain reaction. The results demonstrated stimulation-dependent up-regulations in almost all tissues examined, which indicates that T cells may play important roles in preventing mandarin fish from bacterial invasion. In particular, apart from thymus, T cells were distributed mainly in gill and intestine, where striking up-regulation of C gamma was also observed. These results will facilitate functional studies of teleost TCRs and T cells.; In this study, the constant-region genes (C alpha, C beta and C gamma) that encode the T-cell antigen receptor (TCR) alpha, beta and gamma chains were cloned from mandarin fish, Siniperca chuatsi Basilewsky, an important freshwater fish species in China. The complementary DNA sequences of C alpha, C beta and C gamma were 843, 716 and 906base pairs (bp) in length and had a 465-, 289- and 360-bp 3 ' untranslated region, encoding 125, 142 and 182 amino acids, respectively. The amino-acid sequences of the constant regions of mandarin fish TCR alpha, beta and gamma chains (encoded by C alpha, C beta and C gamma, respectively) were most similar to those of their teleost counterparts, showing 60% similarity with pufferfish, 48% similarity with Atlantic salmon and 57% similarity with flounder, respectively. The phylogenetic analysis revealed that the mandarin fish C alpha, C beta and C gamma were clustered, respectively, with their vertebrate counterparts. The mandarin fish C alpha, C beta and C gamma could also be separated into four domains: immunoglobulin; connecting peptide (CP); transmembrane (TM); and cytoplasmic tail. Several conserved features in mammalian TCRs were also found in those of mandarin fish, such as a conserved cysteine residue in the CP domain of C alpha, necessary for creating an interchain disulphide bond with the TCR beta chain, and a conserved antigen receptor TM motif in C alpha and C beta. Meanwhile, transcripts of C alpha, C beta and C gamma were detectable in all examined organs, with a stronger signal observed in lymphoid organs. In addition, the temporal transcriptional changes for C alpha and C gamma were investigated, 1, 2, 3, 4, 5, 6 and 8weeks after stimulation with Flavobacterium columnare, in head kidney, spleen, blood, thymus, gill and intestine, using real-time polymerase chain reaction. The results demonstrated stimulation-dependent up-regulations in almost all tissues examined, which indicates that T cells may play important roles in preventing mandarin fish from bacterial invasion. In particular, apart from thymus, T cells were distributed mainly in gill and intestine, where striking up-regulation of C gamma was also observed. These results will facilitate functional studies of teleost TCRs and T cells.
SubtypeArticle
KeywordTcr Siniperca Chuatsi Mandarin Fish Flavobacterium Columnare
Department[Tian, J. Y.] Natl Oceanog Ctr, Qingdao, Shandong, Peoples R China ; [Qi, Z. T.] Yancheng Inst Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng, Jiangsu, Peoples R China ; [Wu, N.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Mol Immunol, Wuhan 430072, Hubei Province, Peoples R China ; [Chang, M. X. ; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
DOI10.1111/jfd.12042
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000334388300002
WOS KeywordTELEOST FISH ; TCR ALPHA ; RAINBOW-TROUT ; AEROMONAS-HYDROPHILA ; GENOMIC ORGANIZATION ; MOLECULAR-CLONING ; IMMUNE-RESPONSE ; SPARUS-AURATA ; COMMON CARP ; DELTA CELLS
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] ; National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001]
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20050
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Natl Oceanog Ctr, Qingdao, Shandong, Peoples R China
2.Yancheng Inst Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng, Jiangsu, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Mol Immunol, Wuhan 430072, Hubei Province, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Tian, J. Y.,Qi, Z. T.,Wu, N.,et al. Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare[J]. JOURNAL OF FISH DISEASES,2014,37(2):89-101.
APA Tian, J. Y.,Qi, Z. T.,Wu, N.,Chang, M. X.,Nie, P.,&Nie, P .(2014).Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare.JOURNAL OF FISH DISEASES,37(2),89-101.
MLA Tian, J. Y.,et al."Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare".JOURNAL OF FISH DISEASES 37.2(2014):89-101.
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