| Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala | |
| Zeng, Cong1,3; Liu, Xiao-lian1,4; Wang, Wei-min1; Tong, Jin-gou2; Luo, Wei1; Zhang, Jie1; Gao, Ze-xia1; Gao, ZX (reprint author), Huazhong Agr Univ, Minist Agr, Lab Freshwater Anim Breeding,Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China. | |
| 2014-02-10 | |
| Source Publication | GENE
 |
| ISSN | 0378-1119 |
| Volume | 535Issue:2Pages:239-249 |
| Abstract | In order to test the hypothesis that the expression levels of GH/IGF axis genes would be negatively related to the expression of the myostatin genes in fish species, we cloned six growth regulating genes including growth hormone receptors-1/-2 (GHRs), insulin-like growth factors-I/-II (IGFs) and myostatins-a/-b (MSTNs) from blunt snout bream (Megalobrama amblycephala). The contents of mRNA transcripts for the six genes were determined in the different tissues of adult and developmental stages for the embryonic and larval periods. The results of quantitative real-time PCR showed that GHRs, IGFs and MSTNs were widely expressed in the tissues we tested, with the relatively lower expression levels in mesonephros, gonad and spleen for the six genes. The analysis of expression correlation coefficients among these six genes showed that GHR 1, GHR 2 and MSTN b were correlated with each other in adult tissues (P < 0.01). For the developmental stages, GHR I had a similar expression pattern to GHR 2 during the examined periods, both with the highest expression levels at 160 hpf (hours post-fertilization) (P < 0.05). IGF-II had higher expression levels than that of IGF-I before 400 hpf (P < 0.05), while IGF-I was active after 52 hpf. The maximum of MSTN a and MSTN b mRNA levels were at 24 hpf and 400 hpf, respectively. The analysis of expression correlation coefficients showed that GHR 1, GHR 2, IGF-I, IGF-II and MSTN b were positively correlated with each other during embryonic development (P < 0.01). The results from this study suggested that the relationship between GH/IGF axis genes and MSTNs was complex and not absolutely negative correlated in fish species. (C) 2013 Elsevier B.V. All rights reserved.; In order to test the hypothesis that the expression levels of GH/IGF axis genes would be negatively related to the expression of the myostatin genes in fish species, we cloned six growth regulating genes including growth hormone receptors-1/-2 (GHRs), insulin-like growth factors-I/-II (IGFs) and myostatins-a/-b (MSTNs) from blunt snout bream (Megalobrama amblycephala). The contents of mRNA transcripts for the six genes were determined in the different tissues of adult and developmental stages for the embryonic and larval periods. The results of quantitative real-time PCR showed that GHRs, IGFs and MSTNs were widely expressed in the tissues we tested, with the relatively lower expression levels in mesonephros, gonad and spleen for the six genes. The analysis of expression correlation coefficients among these six genes showed that GHR 1, GHR 2 and MSTN b were correlated with each other in adult tissues (P < 0.01). For the developmental stages, GHR I had a similar expression pattern to GHR 2 during the examined periods, both with the highest expression levels at 160 hpf (hours post-fertilization) (P < 0.05). IGF-II had higher expression levels than that of IGF-I before 400 hpf (P < 0.05), while IGF-I was active after 52 hpf. The maximum of MSTN a and MSTN b mRNA levels were at 24 hpf and 400 hpf, respectively. The analysis of expression correlation coefficients showed that GHR 1, GHR 2, IGF-I, IGF-II and MSTN b were positively correlated with each other during embryonic development (P < 0.01). The results from this study suggested that the relationship between GH/IGF axis genes and MSTNs was complex and not absolutely negative correlated in fish species. (C) 2013 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Megalobrama Amblycephala Ghrs Igfs Mstns Cloning Expression Relationships |
| Department | [Zeng, Cong ; Liu, Xiao-lian ; Wang, Wei-min ; Luo, Wei ; Zhang, Jie ; Gao, Ze-xia] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Lab Freshwater Anim Breeding,Minist Educ,Minist A, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China ; [Tong, Jin-gou] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; [Zeng, Cong] Victoria Univ Wellington, Sch Biol Sci, Wellington 6140, New Zealand ; [Liu, Xiao-lian] Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China |
| DOI | 10.1016/j.gene.2013.11.027 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000331672200023 |
| WOS Keyword | GROWTH-HORMONE-RECEPTOR ; TROUT ONCORHYNCHUS-MYKISS ; CATFISH ICTALURUS-PUNCTATUS ; MESSENGER-RNA EXPRESSION ; FACTOR-BINDING PROTEIN-3 ; ZEBRAFISH DANIO-RERIO ; SKELETAL-MUSCLE ; SPARUS-AURATA ; TISSUE DISTRIBUTION ; CELL-PROLIFERATION |
| Funding Organization | National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20049 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Gao, ZX (reprint author), Huazhong Agr Univ, Minist Agr, Lab Freshwater Anim Breeding,Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China. |
| Affiliation | 1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Lab Freshwater Anim Breeding,Minist Educ,Minist A, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Victoria Univ Wellington, Sch Biol Sci, Wellington 6140, New Zealand 4.Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China |
| Recommended Citation GB/T 7714 | Zeng, Cong,Liu, Xiao-lian,Wang, Wei-min,et al. Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala[J]. GENE,2014,535(2):239-249. |
| APA | Zeng, Cong.,Liu, Xiao-lian.,Wang, Wei-min.,Tong, Jin-gou.,Luo, Wei.,...&Gao, ZX .(2014).Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala.GENE,535(2),239-249. |
| MLA | Zeng, Cong,et al."Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala".GENE 535.2(2014):239-249. |
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