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Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala
Zeng, Cong1,3; Liu, Xiao-lian1,4; Wang, Wei-min1; Tong, Jin-gou2; Luo, Wei1; Zhang, Jie1; Gao, Ze-xia1; Gao, ZX (reprint author), Huazhong Agr Univ, Minist Agr, Lab Freshwater Anim Breeding,Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China.
2014-02-10
Source PublicationGENE
ISSN0378-1119
Volume535Issue:2Pages:239-249
AbstractIn order to test the hypothesis that the expression levels of GH/IGF axis genes would be negatively related to the expression of the myostatin genes in fish species, we cloned six growth regulating genes including growth hormone receptors-1/-2 (GHRs), insulin-like growth factors-I/-II (IGFs) and myostatins-a/-b (MSTNs) from blunt snout bream (Megalobrama amblycephala). The contents of mRNA transcripts for the six genes were determined in the different tissues of adult and developmental stages for the embryonic and larval periods. The results of quantitative real-time PCR showed that GHRs, IGFs and MSTNs were widely expressed in the tissues we tested, with the relatively lower expression levels in mesonephros, gonad and spleen for the six genes. The analysis of expression correlation coefficients among these six genes showed that GHR 1, GHR 2 and MSTN b were correlated with each other in adult tissues (P < 0.01). For the developmental stages, GHR I had a similar expression pattern to GHR 2 during the examined periods, both with the highest expression levels at 160 hpf (hours post-fertilization) (P < 0.05). IGF-II had higher expression levels than that of IGF-I before 400 hpf (P < 0.05), while IGF-I was active after 52 hpf. The maximum of MSTN a and MSTN b mRNA levels were at 24 hpf and 400 hpf, respectively. The analysis of expression correlation coefficients showed that GHR 1, GHR 2, IGF-I, IGF-II and MSTN b were positively correlated with each other during embryonic development (P < 0.01). The results from this study suggested that the relationship between GH/IGF axis genes and MSTNs was complex and not absolutely negative correlated in fish species. (C) 2013 Elsevier B.V. All rights reserved.; In order to test the hypothesis that the expression levels of GH/IGF axis genes would be negatively related to the expression of the myostatin genes in fish species, we cloned six growth regulating genes including growth hormone receptors-1/-2 (GHRs), insulin-like growth factors-I/-II (IGFs) and myostatins-a/-b (MSTNs) from blunt snout bream (Megalobrama amblycephala). The contents of mRNA transcripts for the six genes were determined in the different tissues of adult and developmental stages for the embryonic and larval periods. The results of quantitative real-time PCR showed that GHRs, IGFs and MSTNs were widely expressed in the tissues we tested, with the relatively lower expression levels in mesonephros, gonad and spleen for the six genes. The analysis of expression correlation coefficients among these six genes showed that GHR 1, GHR 2 and MSTN b were correlated with each other in adult tissues (P < 0.01). For the developmental stages, GHR I had a similar expression pattern to GHR 2 during the examined periods, both with the highest expression levels at 160 hpf (hours post-fertilization) (P < 0.05). IGF-II had higher expression levels than that of IGF-I before 400 hpf (P < 0.05), while IGF-I was active after 52 hpf. The maximum of MSTN a and MSTN b mRNA levels were at 24 hpf and 400 hpf, respectively. The analysis of expression correlation coefficients showed that GHR 1, GHR 2, IGF-I, IGF-II and MSTN b were positively correlated with each other during embryonic development (P < 0.01). The results from this study suggested that the relationship between GH/IGF axis genes and MSTNs was complex and not absolutely negative correlated in fish species. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMegalobrama Amblycephala Ghrs Igfs Mstns Cloning Expression Relationships
Department[Zeng, Cong ; Liu, Xiao-lian ; Wang, Wei-min ; Luo, Wei ; Zhang, Jie ; Gao, Ze-xia] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Lab Freshwater Anim Breeding,Minist Educ,Minist A, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China ; [Tong, Jin-gou] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; [Zeng, Cong] Victoria Univ Wellington, Sch Biol Sci, Wellington 6140, New Zealand ; [Liu, Xiao-lian] Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China
DOI10.1016/j.gene.2013.11.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000331672200023
WOS KeywordGROWTH-HORMONE-RECEPTOR ; TROUT ONCORHYNCHUS-MYKISS ; CATFISH ICTALURUS-PUNCTATUS ; MESSENGER-RNA EXPRESSION ; FACTOR-BINDING PROTEIN-3 ; ZEBRAFISH DANIO-RERIO ; SKELETAL-MUSCLE ; SPARUS-AURATA ; TISSUE DISTRIBUTION ; CELL-PROLIFERATION
Funding OrganizationNational Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023] ; National Natural Science Foundation of China [31201988]; Excellent Youth Foundation of Hubei Scientific Committee [2013CFA032]; Modern Agriculture Industry Technology System Construction Projects of China titled as Staple Freshwater Fishes Industry Technology System [CARS-46-05]; National Ministry of Science and Technology Support Program [2012BAD26B00]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2012FB06]; Fundamental Research Funds for the Central Universities [2013PY066, 2011PY023]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20049
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGao, ZX (reprint author), Huazhong Agr Univ, Minist Agr, Lab Freshwater Anim Breeding,Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China.
Affiliation1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Lab Freshwater Anim Breeding,Minist Educ,Minist A, Key Lab Agr Anim Genet Breeding & Reprod,Coll Fis, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Victoria Univ Wellington, Sch Biol Sci, Wellington 6140, New Zealand
4.Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Cong,Liu, Xiao-lian,Wang, Wei-min,et al. Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala[J]. GENE,2014,535(2):239-249.
APA Zeng, Cong.,Liu, Xiao-lian.,Wang, Wei-min.,Tong, Jin-gou.,Luo, Wei.,...&Gao, ZX .(2014).Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala.GENE,535(2),239-249.
MLA Zeng, Cong,et al."Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala".GENE 535.2(2014):239-249.
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