IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis
Fu, Jian Ping1,2; Chen, Shan Nan1; Zou, Peng Fei1; Huang, Bei3; Guo, Zheng1; Zeng, Ling Bing4; Qin, Qi Wei5; Nie, Pin1,3; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2014-03-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume43Issue:1Pages:87-95
AbstractThe IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved.; The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIfn-gamma Ifn-gamma Stimulated Gene Iridovirus Antiviral Activity Turtle
Department[Fu, Jian Ping ; Chen, Shan Nan ; Zou, Peng Fei ; Guo, Zheng ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Huang, Bei ; Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China ; [Zeng, Ling Bing] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei Province, Peoples R China ; [Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
DOI10.1016/j.dci.2013.11.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000331479800012
WOS KeywordNUCLEAR-LOCALIZATION SEQUENCE ; TROUT ONCORHYNCHUS-MYKISS ; DOUBLE-STRANDED-RNA ; INTERFERON-GAMMA ; ANTIVIRAL ACTIVITY ; TELEOST FISH ; BODY PLAN ; II IFNS ; EXPRESSION ; EVOLUTION
Funding OrganizationNational Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703]
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20048
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei Province, Peoples R China
5.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Fu, Jian Ping,Chen, Shan Nan,Zou, Peng Fei,et al. IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):87-95.
APA Fu, Jian Ping.,Chen, Shan Nan.,Zou, Peng Fei.,Huang, Bei.,Guo, Zheng.,...&Nie, P .(2014).IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),87-95.
MLA Fu, Jian Ping,et al."IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):87-95.
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