Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia
Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要:
Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.
Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia.pdf（1455KB）
1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA 2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation:
Geng, Xin; Feng, Jianbin; Liu, Shikai; Wang, Yaping; Arias, Covadonga; Liu, Zhanjiang.Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,169():38-50
Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia.pdf
