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题名: Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia
作者: Geng, Xin1, 2; Feng, Jianbin1, 2; Liu, Shikai1, 2; Wang, Yaping1, 2, 3; Arias, Covadonga1, 2; Liu, Zhanjiang1, 2
通讯作者: Liu, ZJ (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA.
关键词: Fish ; Gene ; Hypoxia ; HIF ; FIH-1
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2014-03-01
DOI: 10.1016/j.cbpb.2013.12.007
卷: 169, 页:38-50
收录类别: SCI
文章类型: Article
部门归属: [Geng, Xin ; Feng, Jianbin ; Liu, Shikai ; Wang, Yaping ; Arias, Covadonga ; Liu, Zhanjiang] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA ; [Geng, Xin ; Feng, Jianbin ; Liu, Shikai ; Wang, Yaping ; Arias, Covadonga ; Liu, Zhanjiang] Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA ; [Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC)
类目[WOS]: Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Zoology
摘要: Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: TUMOR-SUPPRESSOR PROTEIN ; EELPOUT ZOARCES-VIVIPARUS ; MESSENGER-RNA EXPRESSION ; INDUCED GENE-EXPRESSION ; HIPPEL-LINDAU PROTEIN ; REAL-TIME PCR ; OXIDATIVE STRESS ; FACTOR 1-ALPHA ; PROLINE HYDROXYLATION ; PROLYL HYDROXYLATION
语种: 英语
WOS记录号: WOS:000331485100006
ISSN号: 1096-4959
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20047
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA
2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Geng, Xin; Feng, Jianbin; Liu, Shikai; Wang, Yaping; Arias, Covadonga; Liu, Zhanjiang.Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,169():38-50
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