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Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia
Geng, Xin1,2; Feng, Jianbin1,2; Liu, Shikai1,2; Wang, Yaping1,2,3; Arias, Covadonga1,2; Liu, Zhanjiang1,2; Liu, ZJ (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA.
2014-03-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume169Pages:38-50
AbstractHypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.; Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordFish Gene Hypoxia Hif Fih-1
Department[Geng, Xin ; Feng, Jianbin ; Liu, Shikai ; Wang, Yaping ; Arias, Covadonga ; Liu, Zhanjiang] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA ; [Geng, Xin ; Feng, Jianbin ; Liu, Shikai ; Wang, Yaping ; Arias, Covadonga ; Liu, Zhanjiang] Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA ; [Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.cbpb.2013.12.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationAgriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC)
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000331485100006
WOS KeywordTUMOR-SUPPRESSOR PROTEIN ; EELPOUT ZOARCES-VIVIPARUS ; MESSENGER-RNA EXPRESSION ; INDUCED GENE-EXPRESSION ; HIPPEL-LINDAU PROTEIN ; REAL-TIME PCR ; OXIDATIVE STRESS ; FACTOR 1-ALPHA ; PROLINE HYDROXYLATION ; PROLYL HYDROXYLATION
Funding OrganizationAgriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) ; Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC)
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20047
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLiu, ZJ (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA.
Affiliation1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA
2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Geng, Xin,Feng, Jianbin,Liu, Shikai,et al. Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,169:38-50.
APA Geng, Xin.,Feng, Jianbin.,Liu, Shikai.,Wang, Yaping.,Arias, Covadonga.,...&Liu, ZJ .(2014).Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,169,38-50.
MLA Geng, Xin,et al."Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 169(2014):38-50.
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