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Development of 159 transcript-associated microsatellite markers in silver carp (Hypophthalmichthys molitrix)
Feng, Xiu1,2; Yu, Xiaomu1; Fu, Beide3; He, Shunping3; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2014-03-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume6Issue:1Pages:111-113
AbstractThe natural populations of silver carp (Hypophthalmichthys molitrix) have been declining due to habitat fragmentation, over-fishing and water pollution in China. In this study, 159 transcript-associated microsatellites were developed for silver carp by mining transcriptome sequences, and polymorphism of 27 randomly selected loci were further characterized in a wild population. The number of alleles per locus ranged from 4 to 9, the observed and expected heterozygosity ranged from 0.067 to 0.933 and 0.326 to 0.864, respectively. These novel microsatellite markers will be powerful tools to assess the population structure and genetic diversity in silver carp.; The natural populations of silver carp (Hypophthalmichthys molitrix) have been declining due to habitat fragmentation, over-fishing and water pollution in China. In this study, 159 transcript-associated microsatellites were developed for silver carp by mining transcriptome sequences, and polymorphism of 27 randomly selected loci were further characterized in a wild population. The number of alleles per locus ranged from 4 to 9, the observed and expected heterozygosity ranged from 0.067 to 0.933 and 0.326 to 0.864, respectively. These novel microsatellite markers will be powerful tools to assess the population structure and genetic diversity in silver carp.
SubtypeArticle
KeywordSilver Carp Est-ssr Polymorphism
Department[Feng, Xiu ; Yu, Xiaomu ; Tong, Jingou] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Feng, Xiu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Fu, Beide ; He, Shunping] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat CAS, Wuhan 430072, Peoples R China
DOI10.1007/s12686-013-0017-3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20]
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000331708300029
Funding OrganizationNSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20] ; NSFC [31272647]; MOA [200903045]; TGC [TC-12-08-01]; FEBL of China [2011FBZ20]
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20046
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat CAS, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xiu,Yu, Xiaomu,Fu, Beide,et al. Development of 159 transcript-associated microsatellite markers in silver carp (Hypophthalmichthys molitrix)[J]. CONSERVATION GENETICS RESOURCES,2014,6(1):111-113.
APA Feng, Xiu,Yu, Xiaomu,Fu, Beide,He, Shunping,Tong, Jingou,&Tong, JG .(2014).Development of 159 transcript-associated microsatellite markers in silver carp (Hypophthalmichthys molitrix).CONSERVATION GENETICS RESOURCES,6(1),111-113.
MLA Feng, Xiu,et al."Development of 159 transcript-associated microsatellite markers in silver carp (Hypophthalmichthys molitrix)".CONSERVATION GENETICS RESOURCES 6.1(2014):111-113.
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