IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5
Wei, Chang-Yong1,2; Wang, Hou-Peng1; Zhu, Zuo-Yan1; Sun, Yong-Hua1; Sun, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2014-03-07
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
Volume289Issue:10Pages:6604-6618
AbstractBackground: Ventral specification is regulated by Smad1-, Smad5-, and/or Smad9-mediated bone morphogenetic protein (BMP) signaling. Results: Double knockdown instead of single knockdown of smad1 and smad9 induces dorsalization, which cannot be rescued by smad5 overexpression. Conclusion:smad1 and smad9 act redundantly and downstream of smad5 to mediate ventral specification. Significance: The regulation network and cooperative roles of BMP R-Smads in early development are clarified. Bone morphogenetic proteins (BMPs) are multifunctional growth factors that play crucial roles during embryonic development and cell fate determination. Nuclear transduction of BMP signals requires the receptor type Smad proteins, Smad1, Smad5, and Smad9. However, how these Smad proteins cooperate in vivo to regulate various developmental processes is largely unknown. In zebrafish, it was widely believed that the maternally expressed smad5 is essential for dorso-ventral (DV) patterning, and the zygotically transcribed smad1 is not required for normal DV axis establishment. In the present study, we have identified zygotically expressed smad9, which cooperates with smad1 downstream of smad5, to mediate zebrafish early DV patterning in a functional redundant manner. Although knockdown of smad1 or smad9 alone does not lead to visible dorsalization, double knockdown strongly dorsalizes zebrafish embryos, which cannot be efficiently rescued by smad5 overexpression, whereas the dorsalization induced by smad5 knockdown can be fully rescued by overexpression of smad1 or smad9. We have further revealed that the transcription initiations of smad1 and smad9 are repressed by each other, that they are direct transcriptional targets of Smad5, and that smad9, like smad1, is required for myelopoiesis. In conclusion, our study uncovers that smad1 and smad9 act redundantly to each other downstream of smad5 to mediate ventral specification and to regulate embryonic myelopoiesis.; Background: Ventral specification is regulated by Smad1-, Smad5-, and/or Smad9-mediated bone morphogenetic protein (BMP) signaling. Results: Double knockdown instead of single knockdown of smad1 and smad9 induces dorsalization, which cannot be rescued by smad5 overexpression. Conclusion:smad1 and smad9 act redundantly and downstream of smad5 to mediate ventral specification. Significance: The regulation network and cooperative roles of BMP R-Smads in early development are clarified.
SubtypeArticle
KeywordBone Morphogenetic Protein (Bmp) Development Gene Regulation Smad Transcription Factor Zebrafish Dorso-ventral Patterning Myelopoiesis
Department[Wei, Chang-Yong ; Wang, Hou-Peng ; Zhu, Zuo-Yan ; Sun, Yong-Hua] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Wei, Chang-Yong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1074/jbc.M114.549758
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChina 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000332389400025
WOS KeywordDORSOVENTRAL PATTERN-FORMATION ; BETA SIGNAL-TRANSDUCTION ; DORSAL SPINAL-CORD ; DANIO-RERIO ; EMBRYO ; PROTEIN ; GENES ; EXPRESSION ; MOUSE ; DEFECTS
Funding OrganizationChina 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23] ; China 973 Project [2010CB126306, 2012CB944504]; National Natural Science Foundation of China (NSFC) [31222052]; NSFC [30771100]; FEBL Grant [2011FBZ23]
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20045
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorSun, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Chang-Yong,Wang, Hou-Peng,Zhu, Zuo-Yan,et al. Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(10):6604-6618.
APA Wei, Chang-Yong,Wang, Hou-Peng,Zhu, Zuo-Yan,Sun, Yong-Hua,&Sun, YH .(2014).Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5.JOURNAL OF BIOLOGICAL CHEMISTRY,289(10),6604-6618.
MLA Wei, Chang-Yong,et al."Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5".JOURNAL OF BIOLOGICAL CHEMISTRY 289.10(2014):6604-6618.
Files in This Item:
File Name/Size DocType Version Access License
Transcriptional Fact(4347KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wei, Chang-Yong]'s Articles
[Wang, Hou-Peng]'s Articles
[Zhu, Zuo-Yan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wei, Chang-Yong]'s Articles
[Wang, Hou-Peng]'s Articles
[Zhu, Zuo-Yan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wei, Chang-Yong]'s Articles
[Wang, Hou-Peng]'s Articles
[Zhu, Zuo-Yan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.