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Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp
Zhai, Yan-Hua1; Zhou, Li1; Wang, Yang1; Wang, Zhong-Wei1; Li, Zhi1; Zhang, Xiao-Juan1; Gui, Jian-Fang1,2; Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
2014-04-01
Source PublicationPARASITOLOGY RESEARCH
ISSN0932-0113
Volume113Issue:4Pages:1331-1341
AbstractSome myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease.; Some myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease.
SubtypeArticle
KeywordCarassius-auratus-gibelio Apolipoprotein-a-i Antimicrobial Peptides Antiinfective Activity Primary Defense Molecular-data Myxozoa Bloch Cell Identification
Department[Zhai, Yan-Hua ; Zhou, Li ; Wang, Yang ; Wang, Zhong-Wei ; Li, Zhi ; Zhang, Xiao-Juan ; Gui, Jian-Fang] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
DOI10.1007/s00436-014-3772-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22]
Indexed BySCI
Language英语
WOS Research AreaParasitology
WOS SubjectParasitology
WOS IDWOS:000333029800010
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; APOLIPOPROTEIN-A-I ; MYXOZOA MYXOSPOREA ; ANTIMICROBIAL PEPTIDES ; ANTIINFECTIVE ACTIVITY ; PRIMARY DEFENSE ; MOLECULAR-DATA ; BLOCH ; CELL ; IDENTIFICATION
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20044
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhai, Yan-Hua,Zhou, Li,Wang, Yang,et al. Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp[J]. PARASITOLOGY RESEARCH,2014,113(4):1331-1341.
APA Zhai, Yan-Hua.,Zhou, Li.,Wang, Yang.,Wang, Zhong-Wei.,Li, Zhi.,...&Gui, JF .(2014).Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp.PARASITOLOGY RESEARCH,113(4),1331-1341.
MLA Zhai, Yan-Hua,et al."Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp".PARASITOLOGY RESEARCH 113.4(2014):1331-1341.
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