中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp
作者: Zhai, Yan-Hua1; Zhou, Li1; Wang, Yang1; Wang, Zhong-Wei1; Li, Zhi1; Zhang, Xiao-Juan1; Gui, Jian-Fang1, 2
通讯作者: Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
关键词: CARASSIUS-AURATUS-GIBELIO ; APOLIPOPROTEIN-A-I ; ANTIMICROBIAL PEPTIDES ; ANTIINFECTIVE ACTIVITY ; PRIMARY DEFENSE ; MOLECULAR-DATA ; MYXOZOA ; BLOCH ; CELL ; IDENTIFICATION
刊名: PARASITOLOGY RESEARCH
发表日期: 2014-04-01
DOI: 10.1007/s00436-014-3772-5
卷: 113, 期:4, 页:1331-1341
收录类别: SCI
文章类型: Article
部门归属: [Zhai, Yan-Hua ; Zhou, Li ; Wang, Yang ; Wang, Zhong-Wei ; Li, Zhi ; Zhang, Xiao-Juan ; Gui, Jian-Fang] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22]
类目[WOS]: Parasitology
研究领域[WOS]: Parasitology
摘要: Some myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease.
英文摘要: Some myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease.
关键词[WOS]: CARASSIUS-AURATUS-GIBELIO ; APOLIPOPROTEIN-A-I ; MYXOZOA MYXOSPOREA ; ANTIMICROBIAL PEPTIDES ; ANTIINFECTIVE ACTIVITY ; PRIMARY DEFENSE ; MOLECULAR-DATA ; BLOCH ; CELL ; IDENTIFICATION
语种: 英语
WOS记录号: WOS:000333029800010
ISSN号: 0932-0113
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20044
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp.pdf(1557KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Zhai, Yan-Hua; Zhou, Li; Wang, Yang; Wang, Zhong-Wei; Li, Zhi; Zhang, Xiao-Juan; Gui, Jian-Fang.Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp,PARASITOLOGY RESEARCH,2014,113(4):1331-1341
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhai, Yan-Hua]'s Articles
[Zhou, Li]'s Articles
[Wang, Yang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhai, Yan-Hua]‘s Articles
[Zhou, Li]‘s Articles
[Wang, Yang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace