IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis
Zhong, J-X; Zhou, L.; Li, Z.; Gui, J-F; Gui, JF (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2014-06-01
Source PublicationCELL DEATH AND DIFFERENTIATION
ISSN1350-9047
Volume21Issue:6Pages:1013-1024
AbstractNoxa functions in apoptosis and immune system of vertebrates, but its activities in embryo development remain unclear. In this study, we have studied the role of zebrafish Noxa (zNoxa) by using zNoxa-specifc morpholino knockdown and overexpression approaches in developing zebrafish embryos. Expression pattern analysis indicates that zNoxa transcript is of maternal origin, which displays a uniform distribution in early embryonic development until shield stage, and the zygote zNoxa transcription is initiated from this stage and mainly localized in YSL of the embryos. The zNoxa expression alterations result in strong embryonic development defects, demonstrating that zNoxa regulates apoptosis from 75% epiboly stage of development onward, in which zNoxa firstly induces the expression of zBik, and then cooperates with zBik to regulate apoptosis. Moreover, zNoxa knockdown also causes a reduction in number of mitotic cells before 8 h.p.f., suggesting that zNoxa also promotes mitosis before 75% epiboly stage. The effect of zNoxa on mitosis is mediated by zWnt4b in early embryos, whereas zMcl1a and zMcl1b suppress the ability of zNoxa to regulate mitosis and apoptosis at different developmental stages. In addition, mammalian mouse Noxa (mNoxa) mRNA was demonstrated to rescue the arrest of mitosis when zNoxa was knocked down, suggesting that mouse and zebrafish Noxa might have similar dual functions. Therefore, the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.; Noxa functions in apoptosis and immune system of vertebrates, but its activities in embryo development remain unclear. In this study, we have studied the role of zebrafish Noxa (zNoxa) by using zNoxa-specifc morpholino knockdown and overexpression approaches in developing zebrafish embryos. Expression pattern analysis indicates that zNoxa transcript is of maternal origin, which displays a uniform distribution in early embryonic development until shield stage, and the zygote zNoxa transcription is initiated from this stage and mainly localized in YSL of the embryos. The zNoxa expression alterations result in strong embryonic development defects, demonstrating that zNoxa regulates apoptosis from 75% epiboly stage of development onward, in which zNoxa firstly induces the expression of zBik, and then cooperates with zBik to regulate apoptosis. Moreover, zNoxa knockdown also causes a reduction in number of mitotic cells before 8 h.p.f., suggesting that zNoxa also promotes mitosis before 75% epiboly stage. The effect of zNoxa on mitosis is mediated by zWnt4b in early embryos, whereas zMcl1a and zMcl1b suppress the ability of zNoxa to regulate mitosis and apoptosis at different developmental stages. In addition, mammalian mouse Noxa (mNoxa) mRNA was demonstrated to rescue the arrest of mitosis when zNoxa was knocked down, suggesting that mouse and zebrafish Noxa might have similar dual functions. Therefore, the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.
SubtypeArticle
KeywordNoxa Bik Wnt4b Mcl1 Mitosis Apoptosis
Department[Zhong, J-X ; Zhou, L. ; Li, Z. ; Gui, J-F] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1038/cdd.2014.22
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000335966900015
WOS KeywordDIFFERENTIAL EXPRESSION ; PARALICHTHYS-OLIVACEUS ; CARASSIUS-AURATUS ; MOLECULAR-CLONING ; SKELETAL-MUSCLE ; BCL-2 FAMILY ; SEX REVERSAL ; CELL-DEATH ; GENE ; PROTEINS
Funding OrganizationNational Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20039
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, JF (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhong, J-X,Zhou, L.,Li, Z.,et al. Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis[J]. CELL DEATH AND DIFFERENTIATION,2014,21(6):1013-1024.
APA Zhong, J-X,Zhou, L.,Li, Z.,Gui, J-F,&Gui, JF .(2014).Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis.CELL DEATH AND DIFFERENTIATION,21(6),1013-1024.
MLA Zhong, J-X,et al."Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis".CELL DEATH AND DIFFERENTIATION 21.6(2014):1013-1024.
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