| Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio) | |
| Chen, Y.1,2; Zhu, X.1; Yang, Y.1; Han, D.1; Jin, J.1; Xie, S.1; Xie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2014-06-01 | |
| Source Publication | AQUACULTURE NUTRITION
 |
| ISSN | 1353-5773 |
| Volume | 20Issue:3Pages:229-241 |
| Abstract | A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.; A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth. |
| Subtype | Article |
| Keyword | Aeromonas Hydrophilia Carassius Auratus Gibelio Immunity Intestine Microbiota Intestine Morphology Lysozyme |
| Department | [Chen, Y.; Zhu, X.; Yang, Y.; Han, D.; Jin, J.; Xie, S.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Y.] Univ Chinese Acad Sci, Beijing, Peoples R China |
| DOI | 10.1111/anu.12069 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000336258300001 |
| WOS Keyword | POLYMORPHONUCLEAR LEUKOCYTES ; EPITHELIAL-CELLS ; CHANNEL CATFISH ; HYBRID TILAPIA ; GERM-FREE ; PROBIOTICS ; FISH ; INFLAMMATION ; ANTIBIOTICS ; ANTIGEN |
| Funding Organization | National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20032 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Y.,Zhu, X.,Yang, Y.,et al. Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)[J]. AQUACULTURE NUTRITION,2014,20(3):229-241. |
| APA | Chen, Y..,Zhu, X..,Yang, Y..,Han, D..,Jin, J..,...&Xie, S .(2014).Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio).AQUACULTURE NUTRITION,20(3),229-241. |
| MLA | Chen, Y.,et al."Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)".AQUACULTURE NUTRITION 20.3(2014):229-241. |
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