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题名: Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)
作者: Chen, Y.1, 2; Zhu, X.1; Yang, Y.1; Han, D.1; Jin, J.1; Xie, S.1
通讯作者: Xie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Aeromonas hydrophilia ; Carassius auratus gibelio ; immunity ; intestine microbiota ; intestine morphology ; lysozyme
刊名: AQUACULTURE NUTRITION
发表日期: 2014-06-01
DOI: 10.1111/anu.12069
卷: 20, 期:3, 页:229-241
收录类别: SCI
文章类型: Article
部门归属: [Chen, Y.; Zhu, X.; Yang, Y.; Han, D.; Jin, J.; Xie, S.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Y.] Univ Chinese Acad Sci, Beijing, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020]
类目[WOS]: Fisheries
研究领域[WOS]: Fisheries
摘要: A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.
英文摘要: A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.
关键词[WOS]: POLYMORPHONUCLEAR LEUKOCYTES ; EPITHELIAL-CELLS ; CHANNEL CATFISH ; HYBRID TILAPIA ; GERM-FREE ; PROBIOTICS ; FISH ; INFLAMMATION ; ANTIBIOTICS ; ANTIGEN
语种: 英语
WOS记录号: WOS:000336258300001
ISSN号: 1353-5773
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20032
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
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