IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)
Chen, Y.1,2; Zhu, X.1; Yang, Y.1; Han, D.1; Jin, J.1; Xie, S.1; Xie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2014-06-01
Source PublicationAQUACULTURE NUTRITION
ISSN1353-5773
Volume20Issue:3Pages:229-241
AbstractA 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.; A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.
SubtypeArticle
KeywordAeromonas Hydrophilia Carassius Auratus Gibelio Immunity Intestine Microbiota Intestine Morphology Lysozyme
Department[Chen, Y.; Zhu, X.; Yang, Y.; Han, D.; Jin, J.; Xie, S.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Y.] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1111/anu.12069
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020]
Indexed BySCI
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000336258300001
WOS KeywordPOLYMORPHONUCLEAR LEUKOCYTES ; EPITHELIAL-CELLS ; CHANNEL CATFISH ; HYBRID TILAPIA ; GERM-FREE ; PROBIOTICS ; FISH ; INFLAMMATION ; ANTIBIOTICS ; ANTIGEN
Funding OrganizationNational Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020] ; National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20032
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chen, Y.,Zhu, X.,Yang, Y.,et al. Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)[J]. AQUACULTURE NUTRITION,2014,20(3):229-241.
APA Chen, Y..,Zhu, X..,Yang, Y..,Han, D..,Jin, J..,...&Xie, S .(2014).Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio).AQUACULTURE NUTRITION,20(3),229-241.
MLA Chen, Y.,et al."Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)".AQUACULTURE NUTRITION 20.3(2014):229-241.
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