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Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins
Zhao, Yanyan1; Xie, Ping1,2; Fan, Huihui2; Zhao, Sujuan1; Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
2014
Source PublicationENVIRONMENTAL TOXICOLOGY
ISSN1520-4081
Volume29Issue:1Pages:30-39
AbstractHepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014.; Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014.
SubtypeArticle
KeywordMicrocystins Mitochondrion Impairment Oxphos System Crucian Carp
Department[Zhao, Yanyan; Xie, Ping; Zhao, Sujuan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Xie, Ping; Fan, Huihui] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1002/tox.20770
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology ; Water Resources
WOS SubjectEnvironmental Sciences ; Toxicology ; Water Resources
WOS IDWOS:000328545400004
WOS KeywordELECTRON-TRANSPORT CHAIN ; EXTRACTED MICROCYSTINS ; INDUCED APOPTOSIS ; CELL-DEATH ; IN-VIVO ; DAMAGE ; LR ; GENERATION ; MUSCLE ; HEART
Funding OrganizationNational Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20031
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yanyan,Xie, Ping,Fan, Huihui,et al. Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins[J]. ENVIRONMENTAL TOXICOLOGY,2014,29(1):30-39.
APA Zhao, Yanyan,Xie, Ping,Fan, Huihui,Zhao, Sujuan,&Xie, P .(2014).Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins.ENVIRONMENTAL TOXICOLOGY,29(1),30-39.
MLA Zhao, Yanyan,et al."Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins".ENVIRONMENTAL TOXICOLOGY 29.1(2014):30-39.
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