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题名: Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins
作者: Zhao, Yanyan1; Xie, Ping1, 2; Fan, Huihui2; Zhao, Sujuan1
通讯作者: Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
关键词: microcystins ; mitochondrion ; impairment ; OXPHOS system ; crucian carp
刊名: ENVIRONMENTAL TOXICOLOGY
发表日期: 2014
DOI: 10.1002/tox.20770
卷: 29, 期:1, 页:30-39
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Yanyan; Xie, Ping; Zhao, Sujuan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Xie, Ping; Fan, Huihui] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者: National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
类目[WOS]: Environmental Sciences ; Toxicology ; Water Resources
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology ; Water Resources
摘要: Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014.
英文摘要: Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014.
关键词[WOS]: ELECTRON-TRANSPORT CHAIN ; EXTRACTED MICROCYSTINS ; INDUCED APOPTOSIS ; CELL-DEATH ; IN-VIVO ; DAMAGE ; LR ; GENERATION ; MUSCLE ; HEART
语种: 英语
WOS记录号: WOS:000328545400004
ISSN号: 1520-4081
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20031
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
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