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Factors influencing the grass carp gut microbiome and its effect on metabolism
Ni, Jiajia1,2,3; Yan, Qingyun1; Yu, Yuhe1; Zhang, Tanglin4; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2014-03-01
Source PublicationFEMS MICROBIOLOGY ECOLOGY
ISSN0168-6496
Volume87Issue:3Pages:704-714
AbstractGut microbiota have attracted extensive attention recently because of their important role in host metabolism, immunity and health maintenance. The present study focused on factors affecting the gut microbiome of grass carp (Ctenopharyngodon idella) and further explored the potential effect of the gut microbiome on metabolism. Totally, 43.39Gb of screened metagenomic sequences obtained from 24 gut samples were fully analysed. We detected 1228 phylotypes (116 Archaea and 1112 Bacteria), most of which belonged to the phyla Firmicutes, Proteobacteria and Fusobacteria. Totally, 41335 of the detected open reading frames (ORFs) were matched to Kyoto Encyclopedia of Genes and Genomes pathways, and carbohydrate and amino acid metabolism was the main matched pathway deduced from the annotated ORFs. Redundancy analysis based on the phylogenetic composition and gene composition of the gut microbiome indicated that gut fullness and feeding (i.e. ryegrass vs. commercial feed, and pond-cultured vs. wild) were significantly related to the gut microbiome. Moreover, many biosynthesis and metabolism pathways of carbohydrates, amino acids and lipids were significantly enhanced by the gut microbiome in ryegrass-fed grass carp. These findings suggest that the metabolic role played by the gut microbiome in grass carp can be affected by feeding. These findings contribute to the field of fish gut microbial ecology and also provide a basis for follow-up functional studies.; Gut microbiota have attracted extensive attention recently because of their important role in host metabolism, immunity and health maintenance. The present study focused on factors affecting the gut microbiome of grass carp (Ctenopharyngodon idella) and further explored the potential effect of the gut microbiome on metabolism. Totally, 43.39Gb of screened metagenomic sequences obtained from 24 gut samples were fully analysed. We detected 1228 phylotypes (116 Archaea and 1112 Bacteria), most of which belonged to the phyla Firmicutes, Proteobacteria and Fusobacteria. Totally, 41335 of the detected open reading frames (ORFs) were matched to Kyoto Encyclopedia of Genes and Genomes pathways, and carbohydrate and amino acid metabolism was the main matched pathway deduced from the annotated ORFs. Redundancy analysis based on the phylogenetic composition and gene composition of the gut microbiome indicated that gut fullness and feeding (i.e. ryegrass vs. commercial feed, and pond-cultured vs. wild) were significantly related to the gut microbiome. Moreover, many biosynthesis and metabolism pathways of carbohydrates, amino acids and lipids were significantly enhanced by the gut microbiome in ryegrass-fed grass carp. These findings suggest that the metabolic role played by the gut microbiome in grass carp can be affected by feeding. These findings contribute to the field of fish gut microbial ecology and also provide a basis for follow-up functional studies.
SubtypeArticle
KeywordGrass Carp Gut Microbiome Metabolism Metagenome
Department[Ni, Jiajia ; Yan, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Ni, Jiajia] Guangdong Inst Microbiol, Guangzhou, Guangdong, Peoples R China ; [Ni, Jiajia] South China, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China ; [Zhang, Tanglin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1111/1574-6941.12256
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMajor State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000332207200013
WOS KeywordBACTERIAL COMMUNITIES ; CTENOPHARYNGODON-IDELLUS ; NEPHROPS-NORVEGICUS ; DIVERSITY ; INTESTINE ; OBESITY ; RUMEN ; DIET ; PERSPECTIVE ; ENTEROTYPES
Funding OrganizationMajor State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07]
Citation statistics
Cited Times:73[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20021
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Guangdong Inst Microbiol, Guangzhou, Guangdong, Peoples R China
3.South China, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,et al. Factors influencing the grass carp gut microbiome and its effect on metabolism[J]. FEMS MICROBIOLOGY ECOLOGY,2014,87(3):704-714.
APA Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,Zhang, Tanglin,&Yu, YH .(2014).Factors influencing the grass carp gut microbiome and its effect on metabolism.FEMS MICROBIOLOGY ECOLOGY,87(3),704-714.
MLA Ni, Jiajia,et al."Factors influencing the grass carp gut microbiome and its effect on metabolism".FEMS MICROBIOLOGY ECOLOGY 87.3(2014):704-714.
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