| Factors influencing the grass carp gut microbiome and its effect on metabolism | |
| Ni, Jiajia1,2,3; Yan, Qingyun1; Yu, Yuhe1; Zhang, Tanglin4; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. | |
| 2014-03-01 | |
| Source Publication | FEMS MICROBIOLOGY ECOLOGY
 |
| ISSN | 0168-6496 |
| Volume | 87Issue:3Pages:704-714 |
| Abstract | Gut microbiota have attracted extensive attention recently because of their important role in host metabolism, immunity and health maintenance. The present study focused on factors affecting the gut microbiome of grass carp (Ctenopharyngodon idella) and further explored the potential effect of the gut microbiome on metabolism. Totally, 43.39Gb of screened metagenomic sequences obtained from 24 gut samples were fully analysed. We detected 1228 phylotypes (116 Archaea and 1112 Bacteria), most of which belonged to the phyla Firmicutes, Proteobacteria and Fusobacteria. Totally, 41335 of the detected open reading frames (ORFs) were matched to Kyoto Encyclopedia of Genes and Genomes pathways, and carbohydrate and amino acid metabolism was the main matched pathway deduced from the annotated ORFs. Redundancy analysis based on the phylogenetic composition and gene composition of the gut microbiome indicated that gut fullness and feeding (i.e. ryegrass vs. commercial feed, and pond-cultured vs. wild) were significantly related to the gut microbiome. Moreover, many biosynthesis and metabolism pathways of carbohydrates, amino acids and lipids were significantly enhanced by the gut microbiome in ryegrass-fed grass carp. These findings suggest that the metabolic role played by the gut microbiome in grass carp can be affected by feeding. These findings contribute to the field of fish gut microbial ecology and also provide a basis for follow-up functional studies.; Gut microbiota have attracted extensive attention recently because of their important role in host metabolism, immunity and health maintenance. The present study focused on factors affecting the gut microbiome of grass carp (Ctenopharyngodon idella) and further explored the potential effect of the gut microbiome on metabolism. Totally, 43.39Gb of screened metagenomic sequences obtained from 24 gut samples were fully analysed. We detected 1228 phylotypes (116 Archaea and 1112 Bacteria), most of which belonged to the phyla Firmicutes, Proteobacteria and Fusobacteria. Totally, 41335 of the detected open reading frames (ORFs) were matched to Kyoto Encyclopedia of Genes and Genomes pathways, and carbohydrate and amino acid metabolism was the main matched pathway deduced from the annotated ORFs. Redundancy analysis based on the phylogenetic composition and gene composition of the gut microbiome indicated that gut fullness and feeding (i.e. ryegrass vs. commercial feed, and pond-cultured vs. wild) were significantly related to the gut microbiome. Moreover, many biosynthesis and metabolism pathways of carbohydrates, amino acids and lipids were significantly enhanced by the gut microbiome in ryegrass-fed grass carp. These findings suggest that the metabolic role played by the gut microbiome in grass carp can be affected by feeding. These findings contribute to the field of fish gut microbial ecology and also provide a basis for follow-up functional studies. |
| Subtype | Article |
| Keyword | Grass Carp Gut Microbiome Metabolism Metagenome |
| Department | [Ni, Jiajia ; Yan, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Ni, Jiajia] Guangdong Inst Microbiol, Guangzhou, Guangdong, Peoples R China ; [Ni, Jiajia] South China, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China ; [Zhang, Tanglin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1111/1574-6941.12256 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000332207200013 |
| WOS Keyword | BACTERIAL COMMUNITIES ; CTENOPHARYNGODON-IDELLUS ; NEPHROPS-NORVEGICUS ; DIVERSITY ; INTESTINE ; OBESITY ; RUMEN ; DIET ; PERSPECTIVE ; ENTEROTYPES |
| Funding Organization | Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084, 31372202]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Youth Innovation Promotion Association, CAS [Y22Z07] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20021 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Guangdong Inst Microbiol, Guangzhou, Guangdong, Peoples R China 3.South China, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,et al. Factors influencing the grass carp gut microbiome and its effect on metabolism[J]. FEMS MICROBIOLOGY ECOLOGY,2014,87(3):704-714. |
| APA | Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,Zhang, Tanglin,&Yu, YH .(2014).Factors influencing the grass carp gut microbiome and its effect on metabolism.FEMS MICROBIOLOGY ECOLOGY,87(3),704-714. |
| MLA | Ni, Jiajia,et al."Factors influencing the grass carp gut microbiome and its effect on metabolism".FEMS MICROBIOLOGY ECOLOGY 87.3(2014):704-714. |
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