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题名: The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos
作者: Zeng, Cheng1; Sun, Hong2; Xie, Ping3; Wang, Jianghua1; Zhang, Guirong1; Chen, Nan1; Yan, Wei5; Li, Guangyu1, 4
通讯作者: Li, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
关键词: MCLR ; Zebrafish ; Embryo ; Apoptosis ; Developmental toxicity
刊名: AQUATIC TOXICOLOGY
发表日期: 2014-04-01
DOI: 10.1016/j.aquatox.2014.01.021
卷: 149, 页:25-32
收录类别: SCI
文章类型: Article
部门归属: [Zeng, Cheng ; Wang, Jianghua ; Zhang, Guirong ; Chen, Nan ; Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Sun, Hong] Hubei Maternal & Child Hlth Hosp, Wuhan 430070, Peoples R China ; [Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Li, Guangyu] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China ; [Yan, Wei] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: We previously demonstrated that cyanobacteria-derived microcystin-leucine-arginine (MCLR) is able to induce developing toxicity, such as malformation, growth delay and also decreased heart rates in zebrafish embryos. However, the molecular mechanisms by which MCLR induces its toxicity during the development of zebrafish remain largely unknown. Here, we evaluate the role of apoptosis in MCLRinduced developmental toxicity. Zebrafish embryos were exposed to various concentrations of MCLR (0, 0.2, 0.5, 2, and 5.0 mg L-1) for 96 h, at which time reactive oxygen species (ROS) was significantly induced in the 2 and 5.0 mg L-1 MCLR exposure groups. Acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labelling (TUNEL) assay showed that MCLR exposure resulted in cell apoptosis. To test the apoptotic pathway, the expression pattern of several apoptoticrelated genes was examined for the level of enzyme activity, gene and protein expression, respectively. The overall results demonstrate that MCLR induced ROS which consequently triggered apoptosis in the heart of developing zebrafish embryos. Our results also indicate that the p53-Bax-Bc1-2 pathway and the caspase-dependent apoptotic pathway play major roles in MCLR-induced apoptosis in the developing embryos. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要: We previously demonstrated that cyanobacteria-derived microcystin-leucine-arginine (MCLR) is able to induce developing toxicity, such as malformation, growth delay and also decreased heart rates in zebrafish embryos. However, the molecular mechanisms by which MCLR induces its toxicity during the development of zebrafish remain largely unknown. Here, we evaluate the role of apoptosis in MCLRinduced developmental toxicity. Zebrafish embryos were exposed to various concentrations of MCLR (0, 0.2, 0.5, 2, and 5.0 mg L-1) for 96 h, at which time reactive oxygen species (ROS) was significantly induced in the 2 and 5.0 mg L-1 MCLR exposure groups. Acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labelling (TUNEL) assay showed that MCLR exposure resulted in cell apoptosis. To test the apoptotic pathway, the expression pattern of several apoptoticrelated genes was examined for the level of enzyme activity, gene and protein expression, respectively. The overall results demonstrate that MCLR induced ROS which consequently triggered apoptosis in the heart of developing zebrafish embryos. Our results also indicate that the p53-Bax-Bc1-2 pathway and the caspase-dependent apoptotic pathway play major roles in MCLR-induced apoptosis in the developing embryos. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: CARP CYPRINUS-CARPIO ; MICROCYSTIN-LR ; DANIO-RERIO ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; CRUDE EXTRACTS ; EUTROPHIC LAKE ; FISH
语种: 英语
WOS记录号: WOS:000334989300004
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20016
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Hubei Maternal & Child Hlth Hosp, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
4.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
5.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China

Recommended Citation:
Zeng, Cheng; Sun, Hong; Xie, Ping; Wang, Jianghua; Zhang, Guirong; Chen, Nan; Yan, Wei; Li, Guangyu.The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos,AQUATIC TOXICOLOGY,2014,149():25-32
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