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The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos
Zeng, Cheng1; Sun, Hong2; Xie, Ping3; Wang, Jianghua1; Zhang, Guirong1; Chen, Nan1; Yan, Wei5; Li, Guangyu1,4; Li, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
2014-04-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume149Pages:25-32
AbstractWe previously demonstrated that cyanobacteria-derived microcystin-leucine-arginine (MCLR) is able to induce developing toxicity, such as malformation, growth delay and also decreased heart rates in zebrafish embryos. However, the molecular mechanisms by which MCLR induces its toxicity during the development of zebrafish remain largely unknown. Here, we evaluate the role of apoptosis in MCLRinduced developmental toxicity. Zebrafish embryos were exposed to various concentrations of MCLR (0, 0.2, 0.5, 2, and 5.0 mg L-1) for 96 h, at which time reactive oxygen species (ROS) was significantly induced in the 2 and 5.0 mg L-1 MCLR exposure groups. Acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labelling (TUNEL) assay showed that MCLR exposure resulted in cell apoptosis. To test the apoptotic pathway, the expression pattern of several apoptoticrelated genes was examined for the level of enzyme activity, gene and protein expression, respectively. The overall results demonstrate that MCLR induced ROS which consequently triggered apoptosis in the heart of developing zebrafish embryos. Our results also indicate that the p53-Bax-Bc1-2 pathway and the caspase-dependent apoptotic pathway play major roles in MCLR-induced apoptosis in the developing embryos. (C) 2014 Elsevier B.V. All rights reserved.; We previously demonstrated that cyanobacteria-derived microcystin-leucine-arginine (MCLR) is able to induce developing toxicity, such as malformation, growth delay and also decreased heart rates in zebrafish embryos. However, the molecular mechanisms by which MCLR induces its toxicity during the development of zebrafish remain largely unknown. Here, we evaluate the role of apoptosis in MCLRinduced developmental toxicity. Zebrafish embryos were exposed to various concentrations of MCLR (0, 0.2, 0.5, 2, and 5.0 mg L-1) for 96 h, at which time reactive oxygen species (ROS) was significantly induced in the 2 and 5.0 mg L-1 MCLR exposure groups. Acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labelling (TUNEL) assay showed that MCLR exposure resulted in cell apoptosis. To test the apoptotic pathway, the expression pattern of several apoptoticrelated genes was examined for the level of enzyme activity, gene and protein expression, respectively. The overall results demonstrate that MCLR induced ROS which consequently triggered apoptosis in the heart of developing zebrafish embryos. Our results also indicate that the p53-Bax-Bc1-2 pathway and the caspase-dependent apoptotic pathway play major roles in MCLR-induced apoptosis in the developing embryos. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMclr Zebrafish Embryo Apoptosis Developmental Toxicity
Department[Zeng, Cheng ; Wang, Jianghua ; Zhang, Guirong ; Chen, Nan ; Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Sun, Hong] Hubei Maternal & Child Hlth Hosp, Wuhan 430070, Peoples R China ; [Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Li, Guangyu] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China ; [Yan, Wei] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China
DOI10.1016/j.aquatox.2014.01.021
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000334989300004
WOS KeywordCARP CYPRINUS-CARPIO ; MICROCYSTIN-LR ; DANIO-RERIO ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; CRUDE EXTRACTS ; EUTROPHIC LAKE ; FISH
Funding OrganizationNational Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249] ; National Nature Science Foundation of China (NSFC) [31200402]; Specialized Research Fund for the Doctoral Programme of Higher Education (SRFDP) [20120146120008]; Fundamental Research Funds for the Central Universities (Programme) [2013 PY037]; State Key Laboratory of Freshwater Ecology and Biotechnology of China [2013FB17]; Natural Science Foundation of Hubei Province of China [2012FFB01401]; NSFC [31000249]
Citation statistics
Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20016
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorLi, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Hubei Maternal & Child Hlth Hosp, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
4.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
5.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Cheng,Sun, Hong,Xie, Ping,et al. The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos[J]. AQUATIC TOXICOLOGY,2014,149:25-32.
APA Zeng, Cheng.,Sun, Hong.,Xie, Ping.,Wang, Jianghua.,Zhang, Guirong.,...&Li, GY .(2014).The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos.AQUATIC TOXICOLOGY,149,25-32.
MLA Zeng, Cheng,et al."The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos".AQUATIC TOXICOLOGY 149(2014):25-32.
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