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VAGUS NERVE STIMULATION ATTENUATES INTESTINAL EPITHELIAL TIGHT JUNCTIONS DISRUPTION IN ENDOTOXEMIC MICE THROUGH alpha 7 NICOTINIC ACETYLCHOLINE RECEPTORS
Zhou, Hui1; Liang, Hui2; Li, Zhi-Feng1; Xiang, Hui1; Liu, Wei3; Li, Jian-Guo1
2013-08-01
Source PublicationSHOCK
ISSN1073-2322
Volume40Issue:2Pages:144-151
AbstractWe tested the effect of vagus nerve stimulation in endotoxin-induced intestinal tight junction injury in mice challenged with lipopolysaccharide (LPS) and examined the role of alpha 7 nicotinic acetylcholine receptors (alpha 7nAchR) in this process. Endotoxemia was induced by intraperitoneal injection of LPS (10 mg/kg) in male Balb/c mice. Samples were collected 12 h after LPS treatment. Endotoxemia was associated with intestinal barrier dysfunction, as evidenced by increased amount of fluorescein isothiocyanate-dextran in circulation. Western blot and immunofluorescence was performed, and the results demonstrated decreased expression of occludin and zonula occludens 1 along intestinal epithelium in endotoxemic mice. The ultrastructure of tight junction was disrupted as shown by transmission electron microscopy, which was associated with increased intestinal permeability. Stimulation of the right cervical vagus nerve ameliorated the damage of tight junction ultrastructure, which was consistent with decreased permeability to fluorescein isothiocyanate-dextran, and also reversed the decreased expression of tight junction proteins occludin and zonula occludens 1. Vagus nerve stimulation inhibited the upregulated activity of myosin light chain kinase and nuclear factor kappa B. In contrast, alpha-bungarotoxin (a specific alpha 7nAchR antagonist, 0.1 mu g/mouse) administered before vagus nerve stimulation significantly abolished these protective effects of vagus nerve stimulation. Our results for the first time confirmed that vagus nerve stimulation attenuated the disruption of tight junction in intestinal epithelium in endotoxemic mice, which was mediated through suppressing translocation of nuclear factor kappa B p65, downregulating myosin light chain kinase, and the alpha 7nAchR may play an important role in this process.; We tested the effect of vagus nerve stimulation in endotoxin-induced intestinal tight junction injury in mice challenged with lipopolysaccharide (LPS) and examined the role of alpha 7 nicotinic acetylcholine receptors (alpha 7nAchR) in this process. Endotoxemia was induced by intraperitoneal injection of LPS (10 mg/kg) in male Balb/c mice. Samples were collected 12 h after LPS treatment. Endotoxemia was associated with intestinal barrier dysfunction, as evidenced by increased amount of fluorescein isothiocyanate-dextran in circulation. Western blot and immunofluorescence was performed, and the results demonstrated decreased expression of occludin and zonula occludens 1 along intestinal epithelium in endotoxemic mice. The ultrastructure of tight junction was disrupted as shown by transmission electron microscopy, which was associated with increased intestinal permeability. Stimulation of the right cervical vagus nerve ameliorated the damage of tight junction ultrastructure, which was consistent with decreased permeability to fluorescein isothiocyanate-dextran, and also reversed the decreased expression of tight junction proteins occludin and zonula occludens 1. Vagus nerve stimulation inhibited the upregulated activity of myosin light chain kinase and nuclear factor kappa B. In contrast, alpha-bungarotoxin (a specific alpha 7nAchR antagonist, 0.1 mu g/mouse) administered before vagus nerve stimulation significantly abolished these protective effects of vagus nerve stimulation. Our results for the first time confirmed that vagus nerve stimulation attenuated the disruption of tight junction in intestinal epithelium in endotoxemic mice, which was mediated through suppressing translocation of nuclear factor kappa B p65, downregulating myosin light chain kinase, and the alpha 7nAchR may play an important role in this process.
SubtypeArticle
KeywordAlpha 7 Nicotinic Acetylcholine Receptor Tight Junction Endotoxemia Myosin Light Chain Kinase Nf-kappa b Alpha-bungarotoxin
Department[Zhou, Hui; Li, Zhi-Feng; Xiang, Hui; Li, Jian-Guo] Wuhan Univ, Zhongnan Hosp, Dept Intens Care Unit, Wuhan 430071, Hubei Province, Peoples R China; [Liang, Hui] Wuhan Univ, Renmin Hosp, Dept Anesthesiol, Wuhan 430071, Hubei Province, Peoples R China; [Liu, Wei] Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Hubei Province, Peoples R China
DOI10.1097/SHK.0b013e318299e9c0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852]
Indexed BySCI
Language英语
WOS Research AreaGeneral & Internal Medicine ; Hematology ; Surgery ; Cardiovascular System & Cardiology
WOS SubjectCritical Care Medicine ; Hematology ; Surgery ; Peripheral Vascular Disease
WOS IDWOS:000330244400011
WOS KeywordCHOLINERGIC ANTIINFLAMMATORY PATHWAY ; LIGHT-CHAIN KINASE ; BACTERIAL TRANSLOCATION ; HEMORRHAGIC-SHOCK ; BARRIER FUNCTION ; UNITED-STATES ; SEVERE SEPSIS ; IN-VIVO ; ACTIVATION ; INJURY
Funding OrganizationNational Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852] ; National Natural Science Foundation of China [30972852]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19987
Collection分析测试中心_期刊论文
Affiliation1.Wuhan Univ, Zhongnan Hosp, Dept Intens Care Unit, Wuhan 430071, Hubei Province, Peoples R China
2.Wuhan Univ, Renmin Hosp, Dept Anesthesiol, Wuhan 430071, Hubei Province, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Hui,Liang, Hui,Li, Zhi-Feng,et al. VAGUS NERVE STIMULATION ATTENUATES INTESTINAL EPITHELIAL TIGHT JUNCTIONS DISRUPTION IN ENDOTOXEMIC MICE THROUGH alpha 7 NICOTINIC ACETYLCHOLINE RECEPTORS[J]. SHOCK,2013,40(2):144-151.
APA Zhou, Hui,Liang, Hui,Li, Zhi-Feng,Xiang, Hui,Liu, Wei,&Li, Jian-Guo.(2013).VAGUS NERVE STIMULATION ATTENUATES INTESTINAL EPITHELIAL TIGHT JUNCTIONS DISRUPTION IN ENDOTOXEMIC MICE THROUGH alpha 7 NICOTINIC ACETYLCHOLINE RECEPTORS.SHOCK,40(2),144-151.
MLA Zhou, Hui,et al."VAGUS NERVE STIMULATION ATTENUATES INTESTINAL EPITHELIAL TIGHT JUNCTIONS DISRUPTION IN ENDOTOXEMIC MICE THROUGH alpha 7 NICOTINIC ACETYLCHOLINE RECEPTORS".SHOCK 40.2(2013):144-151.
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