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Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins
Zhang, De Lu1; Hu, Chun Xiang2; Li, Dun Hai2; Liu, Yong Ding2; Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2013-11-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume144Pages:250-256
AbstractAphanizomenon flos-aquae is a cyanobacterium that is frequently encountered in eutrophic waters worldwide. It is source of neurotoxins known as aphantoxins or paralytic shellfish poisons (PSPs), which present a major threat to the environment and human health. The molecular mechanism of PSP action is known, however the in vivo effects of this neurotoxin on oxidative stress, lipid peroxidation and the antioxidant defense responses in zebrafish brain remain to be understood. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed using high performance liquid chromatography. The major components of the toxins were gonyautoxins 1 and 5 (GTX1 and GTX5, 34.04% and 21.28%, respectively) and neosaxitoxin (neoSTX, 12.77%). Zebrafish (Danio rerio) were injected intraperitoneally with 7.73 mu g/kg (low dose) and 11.13 mu g/kg (high dose) of A. flos-aquae DC-1 aphantoxins. Oxidative stress, lipid peroxidation and antioxidant defense responses in the zebrafish brain were investigated at various timepoints at 1-24 h post-exposure. Aphantoxin exposure was associated with significantly increased (>1-2 tidies) reactive oxygen species (ROS) and malondialdehyde (MDA) in zebrafish brain compared with the controls at 1-12 h postexposure, suggestive of oxidative stress and lipid peroxidation. In contrast, reduced glutathione (GSH) levels in the zebrafish brain exposed to high or low doses of aphantoxins decreased by 44.88% and 41.33%, respectively, after 1-12 h compared with the controls, suggesting that GSH participated in detoxification to ROS and MDA. Further analysis showed a significant increase in the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) compared with the controls, suggesting elimination of oxidative stress by the antioxidant response in zebrafish brain. All these changes were dose and time dependent. These results suggested that aphantoxins or PSPs increased ROS and MDA and decreased GSH in zebrafish brain, and these changes induced oxidative stress. The increased activity of SOD, CAT and GPx demonstrated that these antioxidant enzymes could play important roles in eliminating excess ROS and MDA. These results also suggest that MDA, ROS, GSH and these three antioxidant enzymes in the brain of zebrafish may act as bioindicators for investigating A. flos-aquae DC-1 aphantoxins or PSPs and algal blooms in nature. (C) 2013 Elsevier B.V. All rights reserved.; Aphanizomenon flos-aquae is a cyanobacterium that is frequently encountered in eutrophic waters worldwide. It is source of neurotoxins known as aphantoxins or paralytic shellfish poisons (PSPs), which present a major threat to the environment and human health. The molecular mechanism of PSP action is known, however the in vivo effects of this neurotoxin on oxidative stress, lipid peroxidation and the antioxidant defense responses in zebrafish brain remain to be understood. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed using high performance liquid chromatography. The major components of the toxins were gonyautoxins 1 and 5 (GTX1 and GTX5, 34.04% and 21.28%, respectively) and neosaxitoxin (neoSTX, 12.77%). Zebrafish (Danio rerio) were injected intraperitoneally with 7.73 mu g/kg (low dose) and 11.13 mu g/kg (high dose) of A. flos-aquae DC-1 aphantoxins. Oxidative stress, lipid peroxidation and antioxidant defense responses in the zebrafish brain were investigated at various timepoints at 1-24 h post-exposure. Aphantoxin exposure was associated with significantly increased (>1-2 tidies) reactive oxygen species (ROS) and malondialdehyde (MDA) in zebrafish brain compared with the controls at 1-12 h postexposure, suggestive of oxidative stress and lipid peroxidation. In contrast, reduced glutathione (GSH) levels in the zebrafish brain exposed to high or low doses of aphantoxins decreased by 44.88% and 41.33%, respectively, after 1-12 h compared with the controls, suggesting that GSH participated in detoxification to ROS and MDA. Further analysis showed a significant increase in the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) compared with the controls, suggesting elimination of oxidative stress by the antioxidant response in zebrafish brain. All these changes were dose and time dependent. These results suggested that aphantoxins or PSPs increased ROS and MDA and decreased GSH in zebrafish brain, and these changes induced oxidative stress. The increased activity of SOD, CAT and GPx demonstrated that these antioxidant enzymes could play important roles in eliminating excess ROS and MDA. These results also suggest that MDA, ROS, GSH and these three antioxidant enzymes in the brain of zebrafish may act as bioindicators for investigating A. flos-aquae DC-1 aphantoxins or PSPs and algal blooms in nature. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordDanio Rerio Neurotoxicity Antioxidant Enzymes Malondialdehyde Reactive Oxygen Species Reduced Glutathione
Department[Zhang, De Lu] Wuhan Univ Technol, Coll Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China ; [Hu, Chun Xiang ; Li, Dun Hai ; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2013.10.011
Funding OrganizationNational High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001]
Indexed BySCI
Language英语
WOS IDWOS:000330817200026
WOS KeywordPARALYTIC SHELLFISH TOXINS ; OXIDATIVE STRESS RESPONSES ; MICROCYSTIN-LR ; CYANOBACTERIAL TOXINS ; OREOCHROMIS-NILOTICUS ; LABORATORY CONDITIONS ; DISSOLVED SAXITOXIN ; ATRAZINE EXPOSURE ; FREE-RADICALS ; LAKE DIANCHI
Funding OrganizationNational High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001] ; National High-Tech Research and Development Program of China [2013AA065804]; National Basic Research Programs of China [2008CB418001]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19985
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ Technol, Coll Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Lu,Hu, Chun Xiang,Li, Dun Hai,et al. Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins[J]. AQUATIC TOXICOLOGY,2013,144:250-256.
APA Zhang, De Lu,Hu, Chun Xiang,Li, Dun Hai,Liu, Yong Ding,&Hu, CX .(2013).Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins.AQUATIC TOXICOLOGY,144,250-256.
MLA Zhang, De Lu,et al."Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins".AQUATIC TOXICOLOGY 144(2013):250-256.
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