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Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction
Yang, Yunfeng1; Chen, Jingrong2,3; Qiu, Dongru2,3,4; Zhou, Jizhong1,2,3,5; Yang, YF (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.
2013-11-25
Source PublicationBMC MICROBIOLOGY
ISSN1471-2180
Volume13Pages:-
AbstractBackground: The completion of genome sequencing in a number of Shewanella species, which are most renowned for their metal reduction capacity, offers a basis for comparative studies. Previous work in Shewanella oneidensis MR-1 has indicated that some genes within a cluster (mtrBAC-omcA-mtrFED) were involved in iron reduction. To explore new features of iron reduction pathways, we experimentally analyzed Shewanella putrefaciens W3-18-1 since its gene cluster is considerably different from that of MR-1 in that the gene cluster encodes only four ORFs. Results: Among the gene cluster, two genes (mtrC and undA) were shown to encode c-type cytochromes. The Delta mtrC deletion mutant revealed significant deficiencies in reducing metals of Fe2O3, a-FeO(OH), beta-FeO(OH), ferric citrate, Mn (IV) and Co(III), but not organic compounds. In contrast, no deficiency of metal reduction was observed in the Delta undA deletion mutant. Nonetheless, undA deletion resulted in progressively slower iron reduction in the absence of mtrC and fitness loss under the iron-using condition, which was indicative of a functional role of UndA in iron reduction. Conclusions: These results provide physiological and biochemical evidences that UndA and MtrC of Shewanella putrefaciens W3-18-1 are involved in iron reduction.; Background: The completion of genome sequencing in a number of Shewanella species, which are most renowned for their metal reduction capacity, offers a basis for comparative studies. Previous work in Shewanella oneidensis MR-1 has indicated that some genes within a cluster (mtrBAC-omcA-mtrFED) were involved in iron reduction. To explore new features of iron reduction pathways, we experimentally analyzed Shewanella putrefaciens W3-18-1 since its gene cluster is considerably different from that of MR-1 in that the gene cluster encodes only four ORFs.
SubtypeArticle
KeywordShewanella Putrefaciens W3-18-1 Iron Reduction C-type Cytochrome
Department[Yang, Yunfeng ; Zhou, Jizhong] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China ; [Chen, Jingrong ; Qiu, Dongru ; Zhou, Jizhong] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA ; [Chen, Jingrong ; Qiu, Dongru ; Zhou, Jizhong] Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA ; [Qiu, Dongru] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China ; [Zhou, Jizhong] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
DOI10.1186/1471-2180-13-267
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000328856200001
WOS KeywordMEMBRANE CYTOCHROMES OMCA ; C-TYPE CYTOCHROMES ; ONEIDENSIS MR-1 ; METAL REDUCTION ; SYSTEMS BIOLOGY ; SURFACE ; FUR ; IDENTIFICATION ; SULFATE ; GENOME
Funding OrganizationNational Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program ; National Science Foundation of China [41171201]; National Key Basic Research Program of China [2013CB956601]; United States Department of Energy's Office of Biological and Environmental Research under Genomics: GTL Program through Shewanella Federation; Microbial Genome Program
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19984
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYang, YF (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.
Affiliation1.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
2.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA
3.Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
5.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
Recommended Citation
GB/T 7714
Yang, Yunfeng,Chen, Jingrong,Qiu, Dongru,et al. Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction[J]. BMC MICROBIOLOGY,2013,13:-.
APA Yang, Yunfeng,Chen, Jingrong,Qiu, Dongru,Zhou, Jizhong,&Yang, YF .(2013).Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction.BMC MICROBIOLOGY,13,-.
MLA Yang, Yunfeng,et al."Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction".BMC MICROBIOLOGY 13(2013):-.
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