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题名: Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library
作者: Liu, Hong1, 3; Zheng, Xiaocong1; Zhang, Feng2; Yu, Li1; Zhang, Xiaohua2; Dai, Heping2; Hua, Qunyi1; Shi, Xiujie1; Lan, Wensheng1; Jia, Peng1; Yuan, Li2; Liu, Hong1
通讯作者: Yuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, East Lake Southern Rd 7, Wuhan 430072, Hubei, Peoples R China.
关键词: Phage display ; Single-chain fragment variable ; Spring viraemia of carp virus
刊名: JOURNAL OF VIROLOGICAL METHODS
发表日期: 2013-12-01
DOI: 10.1016/j.jviromet.2013.08.017
卷: 194, 期:1-2, 页:178-184
收录类别: SCI
文章类型: Article
部门归属: [Liu, Hong ; Zheng, Xiaocong ; Yu, Li ; Hua, Qunyi ; Shi, Xiujie ; Lan, Wensheng ; Jia, Peng ; Liu, Hong] Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China ; [Zhang, Feng ; Zhang, Xiaohua ; Dai, Heping ; Yuan, Li] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Liu, Hong] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214]
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
摘要: Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: MEDIATED ISOTHERMAL AMPLIFICATION ; CYPRINUS-CARPIO ; COMMON CARP ; RHABDOVIRUS ; TECHNOLOGY ; SEQUENCE ; CELLS ; SVCV
语种: 英语
WOS记录号: WOS:000328521600028
ISSN号: 0166-0934
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19983
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

Recommended Citation:
Liu, Hong; Zheng, Xiaocong; Zhang, Feng; Yu, Li; Zhang, Xiaohua; Dai, Heping; Hua, Qunyi; Shi, Xiujie; Lan, Wensheng; Jia, Peng; Yuan, Li; Liu, Hong.Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library,JOURNAL OF VIROLOGICAL METHODS,2013,194(41641):178-184
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