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Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library
Liu, Hong1,3; Zheng, Xiaocong1; Zhang, Feng2; Yu, Li1; Zhang, Xiaohua2; Dai, Heping2; Hua, Qunyi1; Shi, Xiujie1; Lan, Wensheng1; Jia, Peng1; Yuan, Li2; Liu, Hong1; Yuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, East Lake Southern Rd 7, Wuhan 430072, Hubei, Peoples R China.
2013-12-01
Source PublicationJOURNAL OF VIROLOGICAL METHODS
ISSN0166-0934
Volume194Issue:1-2Pages:178-184
AbstractAntibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved.; Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPhage Display Single-chain Fragment Variable Spring Viraemia Of Carp Virus
Department[Liu, Hong ; Zheng, Xiaocong ; Yu, Li ; Hua, Qunyi ; Shi, Xiujie ; Lan, Wensheng ; Jia, Peng ; Liu, Hong] Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China ; [Zhang, Feng ; Zhang, Xiaohua ; Dai, Heping ; Yuan, Li] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Liu, Hong] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1016/j.jviromet.2013.08.017
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChina's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
WOS IDWOS:000328521600028
WOS KeywordMEDIATED ISOTHERMAL AMPLIFICATION ; CYPRINUS-CARPIO ; COMMON CARP ; RHABDOVIRUS ; TECHNOLOGY ; SEQUENCE ; CELLS ; SVCV
Funding OrganizationChina's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] ; China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214]
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19983
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, East Lake Southern Rd 7, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hong,Zheng, Xiaocong,Zhang, Feng,et al. Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library[J]. JOURNAL OF VIROLOGICAL METHODS,2013,194(1-2):178-184.
APA Liu, Hong.,Zheng, Xiaocong.,Zhang, Feng.,Yu, Li.,Zhang, Xiaohua.,...&Yuan, L .(2013).Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library.JOURNAL OF VIROLOGICAL METHODS,194(1-2),178-184.
MLA Liu, Hong,et al."Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library".JOURNAL OF VIROLOGICAL METHODS 194.1-2(2013):178-184.
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