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Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay
Guo, Yongyong; Zhou, Bingsheng; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-10-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume142Issue:-Pages:138-145
AbstractThe present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.; The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPentachlorophenol T-screen Assay Zebrafish Larvae Thyroid Endocrine Disruption Hypothalamic-pituitary-thyroid (Hpt) Axis
Department[Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2013.08.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000328093900014
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; HORMONE RECEPTOR ALPHA-1 ; ZEBRAFISH DANIO-RERIO ; PITUITARY-TUMOR CELLS ; RAT GH3 CELLS ; GROWTH-HORMONE ; GENE-EXPRESSION ; XENOPUS-LAEVIS ; T-SCREEN
Funding OrganizationNational Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
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Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19981
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Guo, Yongyong,Zhou, Bingsheng,Zhou, BS . Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay[J]. AQUATIC TOXICOLOGY,2013,142(-):138-145.
APA Guo, Yongyong,Zhou, Bingsheng,&Zhou, BS .(2013).Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay.AQUATIC TOXICOLOGY,142(-),138-145.
MLA Guo, Yongyong,et al."Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay".AQUATIC TOXICOLOGY 142.-(2013):138-145.
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