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题名: Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay
作者: Guo, Yongyong1; Zhou, Bingsheng1
通讯作者: Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Pentachlorophenol ; T-Screen assay ; Zebrafish larvae ; Thyroid endocrine disruption ; Hypothalamic-pituitary-thyroid (HPT) axis
刊名: AQUATIC TOXICOLOGY
发表日期: 2013-10-15
DOI: 10.1016/j.aquatox.2013.08.005
卷: 142, 期:-, 页:138-145
收录类别: SCI
文章类型: Article
部门归属: [Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; HORMONE RECEPTOR ALPHA-1 ; ZEBRAFISH DANIO-RERIO ; PITUITARY-TUMOR CELLS ; RAT GH3 CELLS ; GROWTH-HORMONE ; GENE-EXPRESSION ; XENOPUS-LAEVIS ; T-SCREEN
语种: 英语
WOS记录号: WOS:000328093900014
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19981
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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