| Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay | |
| Guo, Yongyong; Zhou, Bingsheng; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. | |
| 2013-10-15 | |
| Source Publication | AQUATIC TOXICOLOGY
 |
| ISSN | 0166-445X |
| Volume | 142Issue:-Pages:138-145 |
| Abstract | The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.; The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Pentachlorophenol T-screen Assay Zebrafish Larvae Thyroid Endocrine Disruption Hypothalamic-pituitary-thyroid (Hpt) Axis |
| Department | [Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1016/j.aquatox.2013.08.005 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Toxicology |
| WOS Subject | Marine & Freshwater Biology ; Toxicology |
| WOS ID | WOS:000328093900014 |
| WOS Keyword | POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; HORMONE RECEPTOR ALPHA-1 ; ZEBRAFISH DANIO-RERIO ; PITUITARY-TUMOR CELLS ; RAT GH3 CELLS ; GROWTH-HORMONE ; GENE-EXPRESSION ; XENOPUS-LAEVIS ; T-SCREEN |
| Funding Organization | National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19981 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Guo, Yongyong,Zhou, Bingsheng,Zhou, BS . Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay[J]. AQUATIC TOXICOLOGY,2013,142(-):138-145. |
| APA | Guo, Yongyong,Zhou, Bingsheng,&Zhou, BS .(2013).Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay.AQUATIC TOXICOLOGY,142(-),138-145. |
| MLA | Guo, Yongyong,et al."Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay".AQUATIC TOXICOLOGY 142.-(2013):138-145. |
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