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Toxicogenomic Responses of Zebrafish Embryos/Larvae to Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) Reveal Possible Molecular Mechanisms of Developmental Toxicity
Fu, Jie1,2; Han, Jian3; Zhou, Bingsheng3; Gong, Zhiyuan4; Santos, Eduarda M.5; Huo, Xiaojing4; Zheng, Weiling4; Liu, Hongling1,2; Yu, Hongxia1,2; Liu, Chunsheng1,2; Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China.
2013-09-17
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume47Issue:18Pages:10574-10582
AbstractTris(1,3-dichloro-2-propyl) phosphate (TDCPP) is frequently present in indoor dust and can be detected in human milk. In order to evaluate the effects of TDCPP on vertebrate development, zebrafish embryos/larvae were used as an animal model to examine developmental phenotypes and explore possible mechanisms of toxicity by employing microarrays and iTRAQ labeling quantitative proteomics. The results demonstrated that treatment with TDCPP (3 mu M) from 0.75 h postfertilization (hpf) inhibited cell rearrangement at 4 hpf, caused delay in epiboly at 5.7 and 8.5 hpf, and led to abnormal development (e.g., short tail, reduced body size) and lethality between 14 and 45 hpf, which might be related with altered expression of genes regulating embryogenesis. Furthermore, trunk curvature was observed as the main phenotype in 96 hpf zebrafish larvae exposed to 1 or 3 mu M TDCPP, possibly by changing somite formation and expression of proteins related to fast muscle and cartilage development. Collectively, our results suggest that exposure to TDCPP causes developmental toxicity in vertebrates and warrant the need for studies to evaluate the potential health risks of TDCPP to developing human embryos/infants/children, due to its frequent presence in indoor dust and potential for human exposure.; Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is frequently present in indoor dust and can be detected in human milk. In order to evaluate the effects of TDCPP on vertebrate development, zebrafish embryos/larvae were used as an animal model to examine developmental phenotypes and explore possible mechanisms of toxicity by employing microarrays and iTRAQ labeling quantitative proteomics. The results demonstrated that treatment with TDCPP (3 mu M) from 0.75 h postfertilization (hpf) inhibited cell rearrangement at 4 hpf, caused delay in epiboly at 5.7 and 8.5 hpf, and led to abnormal development (e.g., short tail, reduced body size) and lethality between 14 and 45 hpf, which might be related with altered expression of genes regulating embryogenesis. Furthermore, trunk curvature was observed as the main phenotype in 96 hpf zebrafish larvae exposed to 1 or 3 mu M TDCPP, possibly by changing somite formation and expression of proteins related to fast muscle and cartilage development. Collectively, our results suggest that exposure to TDCPP causes developmental toxicity in vertebrates and warrant the need for studies to evaluate the potential health risks of TDCPP to developing human embryos/infants/children, due to its frequent presence in indoor dust and potential for human exposure.
SubtypeArticle
Department[Fu, Jie; Liu, Hongling; Yu, Hongxia; Liu, Chunsheng] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China; [Fu, Jie; Liu, Hongling; Yu, Hongxia; Liu, Chunsheng] Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China; [Han, Jian; Zhou, Bingsheng] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Gong, Zhiyuan; Huo, Xiaojing; Zheng, Weiling] Natl Univ Singapore, Dept Biol Sci, Singapore 119077, Singapore; [Santos, Eduarda M.] Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4PS, Devon, England
DOI10.1021/es401265q
WOS HeadingsScience & Technology ; Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126]
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS SubjectEngineering, Environmental ; Environmental Sciences
WOS IDWOS:000330096000063
WOS KeywordORGANOPHOSPHATE FLAME RETARDANTS ; IMPRINTED GENE NETWORK ; PROTEIN EXPRESSION ; EMBRYONIC GROWTH ; DUST ; EXPOSURE ; HYPOXIA ; SAMPLES ; FAMILY ; RNA
Funding OrganizationNational Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126] ; National Natural Science Foundation of China [21207063]; Environmental Protection Department of Jiangsu Province [2012044]; Fundamental Research Funds for the Central Universities [1127021126]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19978
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China.
Affiliation1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China
3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Natl Univ Singapore, Dept Biol Sci, Singapore 119077, Singapore
5.Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4PS, Devon, England
Recommended Citation
GB/T 7714
Fu, Jie,Han, Jian,Zhou, Bingsheng,et al. Toxicogenomic Responses of Zebrafish Embryos/Larvae to Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) Reveal Possible Molecular Mechanisms of Developmental Toxicity[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2013,47(18):10574-10582.
APA Fu, Jie.,Han, Jian.,Zhou, Bingsheng.,Gong, Zhiyuan.,Santos, Eduarda M..,...&Liu, CS .(2013).Toxicogenomic Responses of Zebrafish Embryos/Larvae to Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) Reveal Possible Molecular Mechanisms of Developmental Toxicity.ENVIRONMENTAL SCIENCE & TECHNOLOGY,47(18),10574-10582.
MLA Fu, Jie,et al."Toxicogenomic Responses of Zebrafish Embryos/Larvae to Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) Reveal Possible Molecular Mechanisms of Developmental Toxicity".ENVIRONMENTAL SCIENCE & TECHNOLOGY 47.18(2013):10574-10582.
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