IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation
He, Li-Bo; Gao, Xiao-Chan; Ke, Fei; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-11-06
Source PublicationVIRUS RESEARCH
ISSN0168-1702
Volume177Issue:2Pages:194-200
AbstractRana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.; Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordConditional Lethal Mutant Rana Grylio Virus (Rgv) Envelope Protein 53r Recombinant Iridovirus Lac Repressor/operator System Gene Expression
Department[He, Li-Bo ; Gao, Xiao-Chan ; Ke, Fei ; Zhang, Qi-Ya] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.virusres.2013.07.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16]
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000328443800010
WOS KeywordGENE-EXPRESSION ; VACCINIA VIRUS ; FISH CELLS ; FAMILY-IRIDOVIRIDAE ; MEMBRANE-PROTEIN ; LCDV-C ; VECTOR ; GENOME ; MORPHOGENESIS ; REPLICATION
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16]
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19976
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
He, Li-Bo,Gao, Xiao-Chan,Ke, Fei,et al. A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation[J]. VIRUS RESEARCH,2013,177(2):194-200.
APA He, Li-Bo,Gao, Xiao-Chan,Ke, Fei,Zhang, Qi-Ya,&Zhang, QY .(2013).A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation.VIRUS RESEARCH,177(2),194-200.
MLA He, Li-Bo,et al."A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation".VIRUS RESEARCH 177.2(2013):194-200.
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