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题名: A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation
作者: He, Li-Bo1; Gao, Xiao-Chan1; Ke, Fei1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Conditional lethal mutant ; Rana grylio virus (RGV) ; Envelope protein 53R ; Recombinant iridovirus ; lac repressor/operator system ; Gene expression
刊名: VIRUS RESEARCH
发表日期: 2013-11-06
DOI: 10.1016/j.virusres.2013.07.016
卷: 177, 期:2, 页:194-200
收录类别: SCI
文章类型: Article
部门归属: [He, Li-Bo ; Gao, Xiao-Chan ; Ke, Fei ; Zhang, Qi-Ya] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16]
类目[WOS]: Virology
研究领域[WOS]: Virology
摘要: Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: GENE-EXPRESSION ; VACCINIA VIRUS ; FISH CELLS ; FAMILY-IRIDOVIRIDAE ; MEMBRANE-PROTEIN ; LCDV-C ; VECTOR ; GENOME ; MORPHOGENESIS ; REPLICATION
语种: 英语
WOS记录号: WOS:000328443800010
ISSN号: 0168-1702
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19976
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
He, Li-Bo; Gao, Xiao-Chan; Ke, Fei; Zhang, Qi-Ya.A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation,VIRUS RESEARCH,2013,177(2):194-200
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