IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families
Zhu, Chuankun1,2; Sun, Yanhong1,2; Yu, Xiaomu1; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2013-12-20
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:12Pages:-
AbstractGene-centromere (G-C) mapping provides insights into structural and behavioural properties of chromosomes. In this study, G-C mapping using microsatellite markers and meiogynogenetic (meiotic gynogenetic) families were performed in bighead carp (Aristichthys nobilis, 2N = 48), which belongs to Cyprinidae. A total of 218 microsatellites were selected across 24 linkage groups (LGs) of a recently well-defined genetic linkage map for bighead carp, with 151 being heterozygous in at least one of six dams in diploid meiogynogenetic families. After tests for Mendelian segregation in two diploid control families, 103 microsatellites were used for G-C distance calculation in 383 gynogens. The second division segregation frequency (y) was computed through half-tetrad analyses, and the values ranged from 0 to 0.97 (mean 0.40). High G-C recombination frequencies (over 0.667) were observed in 18 (17.5%) of the loci examined, which revealed a low level of chiasma interferences compared with other fishes studied previously. Distribution of G-C distances across LGs ranged from 0 cM to 48.5 cM (mean 20 cM) under the assumption of complete interference. All 24 centromeres were localized according to their closest-related microsatellites at 95% confident intervals. The average distance between centromeres and their closest-linked markers was 6.1 cM with 15 out of 24 LGs having a distance below 5 cM. Based on the centromere positions in this study, we proposed a formula of 24 m/sm+24 t/st chromosomes with 92 arms for bighead carp, which was mostly in accordance with a previously reported karyotype for bighead carp (24 m/sm+24 st). These results of centromere localization provide a basic framework and important resources for genetics and comparative genomics studies in bighead carp and its closely-related cyprinid species.; Gene-centromere (G-C) mapping provides insights into structural and behavioural properties of chromosomes. In this study, G-C mapping using microsatellite markers and meiogynogenetic (meiotic gynogenetic) families were performed in bighead carp (Aristichthys nobilis, 2N = 48), which belongs to Cyprinidae. A total of 218 microsatellites were selected across 24 linkage groups (LGs) of a recently well-defined genetic linkage map for bighead carp, with 151 being heterozygous in at least one of six dams in diploid meiogynogenetic families. After tests for Mendelian segregation in two diploid control families, 103 microsatellites were used for G-C distance calculation in 383 gynogens. The second division segregation frequency (y) was computed through half-tetrad analyses, and the values ranged from 0 to 0.97 (mean 0.40). High G-C recombination frequencies (over 0.667) were observed in 18 (17.5%) of the loci examined, which revealed a low level of chiasma interferences compared with other fishes studied previously. Distribution of G-C distances across LGs ranged from 0 cM to 48.5 cM (mean 20 cM) under the assumption of complete interference. All 24 centromeres were localized according to their closest-related microsatellites at 95% confident intervals. The average distance between centromeres and their closest-linked markers was 6.1 cM with 15 out of 24 LGs having a distance below 5 cM. Based on the centromere positions in this study, we proposed a formula of 24 m/sm+24 t/st chromosomes with 92 arms for bighead carp, which was mostly in accordance with a previously reported karyotype for bighead carp (24 m/sm+24 st). These results of centromere localization provide a basic framework and important resources for genetics and comparative genomics studies in bighead carp and its closely-related cyprinid species.
SubtypeArticle
KeywordOyster Crassostrea-gigas Microsatellite Linkage Map Trout Oncorhynchus-mykiss Rainbow-trout Recombination Rates Triploid Families Sea-cucumber Genetic-map Loci Zebrafish
Department[Zhu, Chuankun ; Sun, Yanhong ; Yu, Xiaomu ; Tong, Jingou] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Zhu, Chuankun ; Sun, Yanhong] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1371/journal.pone.0082950
WOS HeadingsScience & Technology
Funding OrganizationNSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000328745100074
WOS KeywordOYSTER CRASSOSTREA-GIGAS ; MICROSATELLITE LINKAGE MAP ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; RECOMBINATION RATES ; TRIPLOID FAMILIES ; SEA-CUCUMBER ; GENETIC-MAP ; LOCI ; ZEBRAFISH
Funding OrganizationNSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] ; NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20]
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19975
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Chuankun,Sun, Yanhong,Yu, Xiaomu,et al. Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families[J]. PLOS ONE,2013,8(12):-.
APA Zhu, Chuankun,Sun, Yanhong,Yu, Xiaomu,Tong, Jingou,&Tong, JG .(2013).Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families.PLOS ONE,8(12),-.
MLA Zhu, Chuankun,et al."Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families".PLOS ONE 8.12(2013):-.
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