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题名: cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides
作者: Citarasu, T.1, 2; Michaelbabu, M.1; Zhou, L.2; Yang, W.2; Gui, J. F.2
通讯作者: Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India.
关键词: Epinephelus coioides ; Vitamin-K Epoxide Reductase ; cDNA cloning
刊名: INDIAN JOURNAL OF GEO-MARINE SCIENCES
发表日期: 2013-09-01
卷: 42, 期:5, 页:573-579
收录类别: SCI
文章类型: Article
部门归属: [Citarasu, T. ; Michaelbabu, M.] Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India ; [Citarasu, T. ; Zhou, L. ; Yang, W. ; Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
资助者: CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy))
类目[WOS]: Oceanography
研究领域[WOS]: Oceanography
摘要: The cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.
英文摘要: The cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.
关键词[WOS]: COMPLETE GENOME SEQUENCE ; WARFARIN SENSITIVITY ; TISSUE DISTRIBUTION ; IDENTIFICATION ; HORMONE
语种: 英语
WOS记录号: WOS:000329600100006
ISSN号: 0379-5136
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19974
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Citarasu, T.; Michaelbabu, M.; Zhou, L.; Yang, W.; Gui, J. F..cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides,INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(5):573-579
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