IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides
Citarasu, T.1,2; Michaelbabu, M.1; Zhou, L.2; Yang, W.2; Gui, J. F.2; Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India.
2013-09-01
Source PublicationINDIAN JOURNAL OF GEO-MARINE SCIENCES
ISSN0379-5136
Volume42Issue:5Pages:573-579
AbstractThe cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.; The cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.
SubtypeArticle
KeywordEpinephelus Coioides Vitamin-k Epoxide Reductase Cdna Cloning
Department[Citarasu, T. ; Michaelbabu, M.] Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India ; [Citarasu, T. ; Zhou, L. ; Yang, W. ; Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationCAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy))
Indexed BySCI
Language英语
WOS Research AreaOceanography
WOS SubjectOceanography
WOS IDWOS:000329600100006
WOS KeywordCOMPLETE GENOME SEQUENCE ; WARFARIN SENSITIVITY ; TISSUE DISTRIBUTION ; IDENTIFICATION ; HORMONE
Funding OrganizationCAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy)) ; CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy))
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19974
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorCitarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India.
Affiliation1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Citarasu, T.,Michaelbabu, M.,Zhou, L.,et al. cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(5):573-579.
APA Citarasu, T.,Michaelbabu, M.,Zhou, L.,Yang, W.,Gui, J. F.,&Citarasu, T .(2013).cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides.INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(5),573-579.
MLA Citarasu, T.,et al."cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.5(2013):573-579.
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