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题名: Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
作者: Fu, Jian Ping1, 2; Luo, Zhang1; Yan, Yang3; Zou, Peng Fei1; Zhang, Shu Huan1; Qin, Qi Wei3; Nie, Pin1, 4
通讯作者: Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
关键词: cell line ; Pelodiscus sinensis ; Chinese soft-shelled turtle ; transfection ; soft-shelled turtle iridovirus ; STIV
刊名: FISH PATHOLOGY
发表日期: 2013-12-01
卷: 48, 期:4, 页:126-134
收录类别: SCI
文章类型: Article
部门归属: [Fu, Jian Ping ; Luo, Zhang ; Zou, Peng Fei ; Zhang, Shu Huan ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yan, Yang ; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703]
类目[WOS]: Fisheries ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Veterinary Sciences
摘要: continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
英文摘要: continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; GREEN SEA-TURTLE ; CHELONIA-MYDAS ; BODY PLAN ; EXPRESSION ; EVOLUTION ; DISEASE ; COMPLEX ; CULTURE
语种: 英语
WOS记录号: WOS:000329960200003
ISSN号: 0388-788X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19973
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
4.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China

Recommended Citation:
Fu, Jian Ping; Luo, Zhang; Yan, Yang; Zou, Peng Fei; Zhang, Shu Huan; Qin, Qi Wei; Nie, Pin.Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication,FISH PATHOLOGY,2013,48(4):126-134
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