IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
Fu, Jian Ping1,2; Luo, Zhang1; Yan, Yang3; Zou, Peng Fei1; Zhang, Shu Huan1; Qin, Qi Wei3; Nie, Pin1,4; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2013-12-01
Source PublicationFISH PATHOLOGY
ISSN0388-788X
Volume48Issue:4Pages:126-134
Abstractcontinuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.; continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
SubtypeArticle
KeywordCell Line Pelodiscus Sinensis Chinese Soft-shelled Turtle Transfection Soft-shelled Turtle Iridovirus Stiv
Department[Fu, Jian Ping ; Luo, Zhang ; Zou, Peng Fei ; Zhang, Shu Huan ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yan, Yang ; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Veterinary Sciences
WOS SubjectFisheries ; Veterinary Sciences
WOS IDWOS:000329960200003
WOS KeywordSMOOTH-MUSCLE-CELLS ; GREEN SEA-TURTLE ; CHELONIA-MYDAS ; BODY PLAN ; EXPRESSION ; EVOLUTION ; DISEASE ; COMPLEX ; CULTURE
Funding OrganizationNational Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703] ; National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19973
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
4.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
Recommended Citation
GB/T 7714
Fu, Jian Ping,Luo, Zhang,Yan, Yang,et al. Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication[J]. FISH PATHOLOGY,2013,48(4):126-134.
APA Fu, Jian Ping.,Luo, Zhang.,Yan, Yang.,Zou, Peng Fei.,Zhang, Shu Huan.,...&Nie, P .(2013).Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication.FISH PATHOLOGY,48(4),126-134.
MLA Fu, Jian Ping,et al."Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication".FISH PATHOLOGY 48.4(2013):126-134.
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