IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish
Dan, Cheng1; Mei, Jie2; Wang, Da1; Gui, Jian-Fang1; Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
2013
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume9Issue:10Pages:1043-1049
AbstractPf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.; Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.
SubtypeArticle
KeywordYellow Catfish Sex-specific Marker Genome Walking Yy Super-males All-male Fish
Department[Dan, Cheng ; Wang, Da ; Gui, Jian-Fang] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Mei, Jie] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
DOI10.7150/ijbs.7203
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organizationspecial Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000330121700004
WOS KeywordSOLE CYNOGLOSSUS-SEMILAEVIS ; PELTEOBAGRUS-FULVIDRACO RICHARDSON ; CARASSIUS-AURATUS GIBELIO ; AFLP-BASED APPROACH ; CYPRINUS-CARPIO ; CLONAL DIVERSITY ; DNA FRAGMENTS ; COMMON CARP ; LINKAGE MAP ; IDENTIFICATION
Funding Organizationspecial Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
Citation statistics
Cited Times:58[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19972
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorMei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
Affiliation1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Dan, Cheng,Mei, Jie,Wang, Da,et al. Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(10):1043-1049.
APA Dan, Cheng,Mei, Jie,Wang, Da,Gui, Jian-Fang,&Mei, J .(2013).Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,9(10),1043-1049.
MLA Dan, Cheng,et al."Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.10(2013):1043-1049.
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