IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Intracellular Interferons in Fish: A Unique Means to Combat Viral Infection
Chang, Ming-Xian1,2; Zou, Jun1; Nie, Pin2; Huang, Bei3; Yu, Zhanglong2; Collet, Bertrand4; Secombes, Chris J.1; Chang, MX (reprint author), Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland.
2013-11-01
Source PublicationPLOS PATHOGENS
ISSN1553-7374
Volume9Issue:11Pages:-
AbstractWe demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection.; We demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection.
SubtypeArticle
KeywordInnate Immune-response Zebrafish Danio-rerio i Interferon Ifn Subtypes Identification Expression Evolution Rna Conservation Vertebrates
DepartmentChang, Ming-Xian; Zou, Jun; Secombes, Chris J.] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland; [Chang, Ming-Xian; Nie, Pin; Yu, Zhanglong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Huang, Bei] Jimei Univ, Coll Fisheries, Xiamen, Peoples R China; [Collet, Bertrand] Marine Scotland Sci Marine Lab, Aberdeen, Scotland
DOI10.1371/journal.ppat.1003736
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Parasitology ; Virology
WOS SubjectMicrobiology ; Parasitology ; Virology
WOS IDWOS:000330386900014
WOS KeywordINNATE IMMUNE-RESPONSE ; ZEBRAFISH DANIO-RERIO ; I INTERFERON ; IFN SUBTYPES ; IDENTIFICATION ; EXPRESSION ; EVOLUTION ; RNA ; CONSERVATION ; VERTEBRATES
Funding OrganizationNational Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project ; National Natural Science Foundation of China [30830083]; National Basic Research Program of China (973 Program) [2009CB118703]; Chinese Academy of Sciences; Royal Society of Edinburgh; EU Imaquanim project
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19970
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChang, MX (reprint author), Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland.
Affiliation1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen, Peoples R China
4.Marine Scotland Sci Marine Lab, Aberdeen, Scotland
Recommended Citation
GB/T 7714
Chang, Ming-Xian,Zou, Jun,Nie, Pin,et al. Intracellular Interferons in Fish: A Unique Means to Combat Viral Infection[J]. PLOS PATHOGENS,2013,9(11):-.
APA Chang, Ming-Xian.,Zou, Jun.,Nie, Pin.,Huang, Bei.,Yu, Zhanglong.,...&Chang, MX .(2013).Intracellular Interferons in Fish: A Unique Means to Combat Viral Infection.PLOS PATHOGENS,9(11),-.
MLA Chang, Ming-Xian,et al."Intracellular Interferons in Fish: A Unique Means to Combat Viral Infection".PLOS PATHOGENS 9.11(2013):-.
Files in This Item:
File Name/Size DocType Version Access License
Intracellular IFN in(1071KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chang, Ming-Xian]'s Articles
[Zou, Jun]'s Articles
[Nie, Pin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chang, Ming-Xian]'s Articles
[Zou, Jun]'s Articles
[Nie, Pin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chang, Ming-Xian]'s Articles
[Zou, Jun]'s Articles
[Nie, Pin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Intracellular IFN in rainbow trout.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.