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题名: Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches
作者: Zhang, Xiang1, 2; Feng, Weisong1; Iran, Qingyun1; Yu, Yuhe1
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
关键词: Free-living ; particle-attached ; DGGE pattern ; 16S rDNA ; bacterial groups
刊名: JOURNAL OF PURE AND APPLIED MICROBIOLOGY
发表日期: 2013-12-01
卷: 7, 期:4, 页:2731-2738
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Xiang ; Feng, Weisong ; Iran, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China ; [Zhang, Xiang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05]
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
摘要: To find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.
英文摘要: To find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.
关键词[WOS]: GRADIENT GEL-ELECTROPHORESIS ; LIVING BACTERIAL COMMUNITIES ; NW MEDITERRANEAN SEA ; 16S RIBOSOMAL-RNA ; ASSEMBLAGES ; DIVERSITY ; LAKE ; VARIABILITY ; DYNAMICS ; RIVER
语种: 英语
WOS记录号: WOS:000331428900030
ISSN号: 0973-7510
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19967
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Xiang; Feng, Weisong; Iran, Qingyun; Yu, Yuhe.Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches,JOURNAL OF PURE AND APPLIED MICROBIOLOGY,2013,7(4):2731-2738
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