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Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches
Zhang, Xiang1,2; Feng, Weisong1; Iran, Qingyun1; Yu, Yuhe1; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
2013-12-01
Source PublicationJOURNAL OF PURE AND APPLIED MICROBIOLOGY
ISSN0973-7510
Volume7Issue:4Pages:2731-2738
AbstractTo find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.; To find out different sampling approaches how to affect DNA fingerprinting, environmental genomic DNA was prepared with three sampling approaches. The DNA profiles of 5 bacterial subgroups (alpha-,beta-,gamma-Proteobacteria, Bacteroidetes and Cyanobacteria) and total bacteria were investigated by using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The DGGE patterns showed alpha-Proteobacteria had more free-living OTUs, while gamma-Proteobacteria and Bacteroidetes had more particle-attached OTUs. Cyanobacteria and beta-Proteobacteria had approximately equal OTU number in the two fractions. Principle component analysis based on DNA banding patterns showed there were obvious differences among free-living, particle-attached fraction and whole bacterial community composition of 3 bacterial subgroups (alpha-, gamma-Proteobacteria and Bacteroidetes) and total bacteria, while the patterns of beta-Proteobacteria and Cyanobacteria were similar, suggesting the dynamics of bacterial subgroups can be better understand by combining different collection approaches.
SubtypeArticle
KeywordFree-living Particle-attached Dgge Pattern 16s Rdna Bacterial Groups
Department[Zhang, Xiang ; Feng, Weisong ; Iran, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China ; [Zhang, Xiang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000331428900030
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; LIVING BACTERIAL COMMUNITIES ; NW MEDITERRANEAN SEA ; 16S RIBOSOMAL-RNA ; ASSEMBLAGES ; DIVERSITY ; LAKE ; VARIABILITY ; DYNAMICS ; RIVER
Funding OrganizationNational Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05] ; National Natural Science Foundation of China [30770298]; Major State Basic Research Development Program of China [2008CB4P8P05]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19967
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiang,Feng, Weisong,Iran, Qingyun,et al. Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches[J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY,2013,7(4):2731-2738.
APA Zhang, Xiang,Feng, Weisong,Iran, Qingyun,Yu, Yuhe,&Yu, YH .(2013).Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches.JOURNAL OF PURE AND APPLIED MICROBIOLOGY,7(4),2731-2738.
MLA Zhang, Xiang,et al."Differences in DNA Fingerprints of Bacterioplankton Community as Affected by Biomass Collection Approaches".JOURNAL OF PURE AND APPLIED MICROBIOLOGY 7.4(2013):2731-2738.
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