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题名: Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803
作者: Liu Lian1; Hu HanHua1; Gao Hong1; Xu XuDong1
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Synechocystis ; glycogen synthesis ; phosphoglucomutase ; phosphomannomutase
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2013-12-01
DOI: 10.1007/s11434-013-5958-0
卷: 58, 期:36, 页:4616-4621
收录类别: SCI
文章类型: Article
部门归属: [Liu Lian ; Hu HanHua ; Gao Hong ; Xu XuDong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology
资助者: National Natural Science Foundation of China [30825003]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.
英文摘要: Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.
关键词[WOS]: BLUE-GREEN-ALGA ; PSEUDOMONAS-AERUGINOSA ; PHOSPHOGLUCOSAMINE MUTASE ; SP PCC-6803 ; CYANOBACTERIA ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; BIOSYNTHESIS ; PURIFICATION ; ANABAENA
语种: 英语
WOS记录号: WOS:000327482400014
ISSN号: 1001-6538
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19788
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Liu Lian; Hu HanHua; Gao Hong; Xu XuDong.Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803,CHINESE SCIENCE BULLETIN,2013,58(36):4616-4621
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