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Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803
Liu Lian; Hu HanHua; Gao Hong; Xu XuDong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-12-01
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume58Issue:36Pages:4616-4621
AbstractPhosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.; Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.
SubtypeArticle
KeywordSynechocystis Glycogen Synthesis Phosphoglucomutase Phosphomannomutase
Department[Liu Lian ; Hu HanHua ; Gao Hong ; Xu XuDong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1007/s11434-013-5958-0
WOS HeadingsScience & Technology
Funding OrganizationNational Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000327482400014
WOS KeywordBLUE-GREEN-ALGA ; PSEUDOMONAS-AERUGINOSA ; PHOSPHOGLUCOSAMINE MUTASE ; SP PCC-6803 ; CYANOBACTERIA ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; BIOSYNTHESIS ; PURIFICATION ; ANABAENA
Funding OrganizationNational Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003] ; National Natural Science Foundation of China [30825003]
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19788
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu Lian,Hu HanHua,Gao Hong,et al. Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803[J]. CHINESE SCIENCE BULLETIN,2013,58(36):4616-4621.
APA Liu Lian,Hu HanHua,Gao Hong,Xu XuDong,&Xu, XD .(2013).Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803.CHINESE SCIENCE BULLETIN,58(36),4616-4621.
MLA Liu Lian,et al."Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803".CHINESE SCIENCE BULLETIN 58.36(2013):4616-4621.
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