中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression
作者: Yue, Huamei1, 3; Ye, Huan1; Chen, Xihua1; Cao, Hong2; Li, Chuangju1, 3
通讯作者: Li, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
关键词: Chinese sturgeon ; GnRH1 ; cGnRH2 ; E2 ; Real-time PCR
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
发表日期: 2013-12-01
DOI: 10.1016/j.cbpa.2013.08.011
卷: 166, 期:4, 页:529-537
收录类别: SCI
文章类型: Article
部门归属: [Yue, Huamei; Ye, Huan; Chen, Xihua; Li, Chuangju] Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China; [Cao, Hong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Yue, Huamei; Li, Chuangju] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403]
类目[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
摘要: The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino adds, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17 beta-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P < 0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino adds, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17 beta-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P < 0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: HYPOTHALAMO-HYPOPHYSEAL SYSTEM ; PITUITARY-GONAD AXIS ; GNRH-II GENE ; FUNCTIONAL EVOLUTION ; 3 FORMS ; FISH ; BRAIN ; RECEPTORS ; CHICKEN ; IDENTIFICATION
语种: 英语
WOS记录号: WOS:000327109400001
ISSN号: 1095-6433
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19785
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression.pdf(1701KB)----开放获取View Download

作者单位: 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yue, Huamei]'s Articles
[Ye, Huan]'s Articles
[Chen, Xihua]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yue, Huamei]‘s Articles
[Ye, Huan]‘s Articles
[Chen, Xihua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace