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Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression
Yue, Huamei1,3; Ye, Huan1; Chen, Xihua1; Cao, Hong2; Li, Chuangju1,3; Li, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
2013-12-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
ISSN1095-6433
Volume166Issue:4Pages:529-537
AbstractThe Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino adds, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17 beta-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P < 0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon. (C) 2013 Elsevier Inc. All rights reserved.; The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino adds, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17 beta-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P < 0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordChinese Sturgeon Gnrh1 Cgnrh2 E2 Real-time Pcr
Department[Yue, Huamei; Ye, Huan; Chen, Xihua; Li, Chuangju] Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China; [Cao, Hong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Yue, Huamei; Li, Chuangju] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
DOI10.1016/j.cbpa.2013.08.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Physiology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Physiology ; Zoology
WOS IDWOS:000327109400001
WOS KeywordHYPOTHALAMO-HYPOPHYSEAL SYSTEM ; PITUITARY-GONAD AXIS ; GNRH-II GENE ; FUNCTIONAL EVOLUTION ; 3 FORMS ; FISH ; BRAIN ; RECEPTORS ; CHICKEN ; IDENTIFICATION
Funding OrganizationNational Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403] ; National Natural Science Foundation of China [30571411, 31172113]; National Nonprofit Institute Research Grant of Chinese Academy of Fisheries [2013A0403]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19785
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLi, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
Affiliation1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
Recommended Citation
GB/T 7714
Yue, Huamei,Ye, Huan,Chen, Xihua,et al. Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2013,166(4):529-537.
APA Yue, Huamei,Ye, Huan,Chen, Xihua,Cao, Hong,Li, Chuangju,&Li, CJ .(2013).Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,166(4),529-537.
MLA Yue, Huamei,et al."Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 166.4(2013):529-537.
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