IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation
Mei, Yun1,2; Yin, Naida1; Jin, Xia1; He, Jiangyan1; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Dept Environm Biol & Human Hlth, 7 South Donghu St, Wuhan 430072, Huibei, Peoples R China.
2013-12-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume154Issue:12Pages:4640-4649
AbstractIt has been reported that various endocrine hormones exert prominent effects on erythropoiesis. We conducted experiments to identify the mechanisms involved in the regulatory functions of adrenergic signaling on erythroid differentiation and the expression of hemoglobin genes. The reactivation of fetal hemoglobin (HbF) expression is also an important therapeutic option in patients with hemoglobin disorders. We determined that the adrenergic agonists phenylephrine (PE) and isoproterenol (ISO) can induce the production of beta-hemoglobin embryonic 1 (hbbe1) mRNA and protein in adult zebrafish erythrocytes. Elevated levels of HbF mRNA and protein were also observed in human K562 cells after the adrenergic agonist treatments. In addition, elevated levels of histone acetylation were observed in both the PE-and the ISO-treated K562 cells. Additionally, our data further indicate that the induction effects of the adrenergic agonists on HbF synthesis and erythroid differentiation in K562 cells are mainly mediated by the p38 MAPK/cAMP response element binding pathway. In summary, the present study identifies the role of the adrenergic agonists PE and ISO on p38 MAPK and ERK signaling for the stimulation of HbF production and erythroid differentiation.; It has been reported that various endocrine hormones exert prominent effects on erythropoiesis. We conducted experiments to identify the mechanisms involved in the regulatory functions of adrenergic signaling on erythroid differentiation and the expression of hemoglobin genes. The reactivation of fetal hemoglobin (HbF) expression is also an important therapeutic option in patients with hemoglobin disorders. We determined that the adrenergic agonists phenylephrine (PE) and isoproterenol (ISO) can induce the production of beta-hemoglobin embryonic 1 (hbbe1) mRNA and protein in adult zebrafish erythrocytes. Elevated levels of HbF mRNA and protein were also observed in human K562 cells after the adrenergic agonist treatments. In addition, elevated levels of histone acetylation were observed in both the PE-and the ISO-treated K562 cells. Additionally, our data further indicate that the induction effects of the adrenergic agonists on HbF synthesis and erythroid differentiation in K562 cells are mainly mediated by the p38 MAPK/cAMP response element binding pathway. In summary, the present study identifies the role of the adrenergic agonists PE and ISO on p38 MAPK and ERK signaling for the stimulation of HbF production and erythroid differentiation.
SubtypeArticle
KeywordGlobin Messenger-rna Gene-expression K562 Cells Activation Zebrafish Induction Stimulation Progenitors Pathways Inducers
Department[Mei, Yun; Yin, Naida; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Huibei, Peoples R China; [Mei, Yun] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1210/en.2013-1535
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000327545200020
WOS KeywordGLOBIN MESSENGER-RNA ; GENE-EXPRESSION ; K562 CELLS ; ACTIVATION ; ZEBRAFISH ; INDUCTION ; STIMULATION ; PROGENITORS ; PATHWAYS ; INDUCERS
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19784
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Dept Environm Biol & Human Hlth, 7 South Donghu St, Wuhan 430072, Huibei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Huibei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Mei, Yun,Yin, Naida,Jin, Xia,et al. The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation[J]. ENDOCRINOLOGY,2013,154(12):4640-4649.
APA Mei, Yun,Yin, Naida,Jin, Xia,He, Jiangyan,Yin, Zhan,&Yin, Z .(2013).The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation.ENDOCRINOLOGY,154(12),4640-4649.
MLA Mei, Yun,et al."The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation".ENDOCRINOLOGY 154.12(2013):4640-4649.
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