| Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi | |
| Yang, Ziyan1,2; Li, Junhua3; Li, Ying1; Wu, Hongjuan1; Wang, Xiaoyan4; Wu, HJ (reprint author), Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China. | |
| 2013-12-01 | |
| Source Publication | MOLECULAR IMMUNOLOGY
 |
| ISSN | 0161-5890 |
| Volume | 56Issue:4Pages:729-738 |
| Abstract | Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In the present study, a short-form PGRP, designated as HcPGRPS1 was identified from freshwater mussel Hyriopsis cumingi. The deduced amino acid sequence of HcPGRPS1 is composed of 235 residues which contains a conserved PGRP domain at the C-terminus. Sequence analysis showed that HcPGRPS1 shared high identities with other known PGRPs. The mRNA of HcPGRPS1 is constitutively expressed in a wide range of all tested tissues, with highest expression level in hepatopancreas, and its expression in tissues (gonad, nephridium, gill and foot) was up-regulated significantly after LPS or PGN stimulation (P<0.05). The recombinant protein of HcPGRPS1 exhibited binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and DAP-PGN from Bacillus subtills. Furthermore, recombinant HcPGRPS1 displayed strong antibacterial activity to both Gram-negative bacteria Escherichia coli, Aeromonas hydrophila, Aeromonas sobria and Gram-positive bacteria S. aureus in the presence of Zn2+. These results suggested that HcPGRPS1 plays a multifunctional role in the defense and protection mechanisms of mussel innate immunity against infections. (C) 2013 Elsevier Ltd. All rights reserved.; Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In the present study, a short-form PGRP, designated as HcPGRPS1 was identified from freshwater mussel Hyriopsis cumingi. The deduced amino acid sequence of HcPGRPS1 is composed of 235 residues which contains a conserved PGRP domain at the C-terminus. Sequence analysis showed that HcPGRPS1 shared high identities with other known PGRPs. The mRNA of HcPGRPS1 is constitutively expressed in a wide range of all tested tissues, with highest expression level in hepatopancreas, and its expression in tissues (gonad, nephridium, gill and foot) was up-regulated significantly after LPS or PGN stimulation (P<0.05). The recombinant protein of HcPGRPS1 exhibited binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and DAP-PGN from Bacillus subtills. Furthermore, recombinant HcPGRPS1 displayed strong antibacterial activity to both Gram-negative bacteria Escherichia coli, Aeromonas hydrophila, Aeromonas sobria and Gram-positive bacteria S. aureus in the presence of Zn2+. These results suggested that HcPGRPS1 plays a multifunctional role in the defense and protection mechanisms of mussel innate immunity against infections. (C) 2013 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Hyriopsis Cumingi Peptidoglycan Recognition Protein Innate Immunity Peptidoglycan-binding Activity Amidase Activity Antibacterial Activity |
| Department | [Yang, Ziyan ; Li, Ying ; Wu, Hongjuan] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China ; [Yang, Ziyan] North China Univ Water Resources & Elect Power, Sch Environm & Municipal Engn, Zhengzhou 450011, Henan Province, Peoples R China ; [Li, Junhua] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Wang, Xiaoyan] Water Resources Off Hubei Prov, Wuhan 430071, Hubei Province, Peoples R China |
| DOI | 10.1016/j.molimm.2013.06.019 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Immunology |
| WOS Subject | Biochemistry & Molecular Biology ; Immunology |
| WOS ID | WOS:000326203300048 |
| WOS Keyword | MESSENGER-RNA EXPRESSION ; GRAM-POSITIVE BACTERIA ; INNATE IMMUNITY ; ANTIBACTERIAL ACTIVITY ; ANOPHELES-GAMBIAE ; CRASSOSTREA-GIGAS ; CHLAMYS-FARRERI ; BOMBYX-MORI ; PGRP GENES ; DROSOPHILA |
| Funding Organization | National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] ; National Natural Science Foundation of China [31072209] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19779 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wu, HJ (reprint author), Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China. |
| Affiliation | 1.Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China 2.North China Univ Water Resources & Elect Power, Sch Environm & Municipal Engn, Zhengzhou 450011, Henan Province, Peoples R China 3.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 4.Water Resources Off Hubei Prov, Wuhan 430071, Hubei Province, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Ziyan,Li, Junhua,Li, Ying,et al. Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi[J]. MOLECULAR IMMUNOLOGY,2013,56(4):729-738. |
| APA | Yang, Ziyan,Li, Junhua,Li, Ying,Wu, Hongjuan,Wang, Xiaoyan,&Wu, HJ .(2013).Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi.MOLECULAR IMMUNOLOGY,56(4),729-738. |
| MLA | Yang, Ziyan,et al."Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi".MOLECULAR IMMUNOLOGY 56.4(2013):729-738. |
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